Three-dimensional imaging of rhodamine 123 fluorescence distribution in human melanoma cells by means of confocal laser scanning microscopy

Cells Tissues Organs

Volumn 157, Issue 2, 1996, Pages 116-125

  Porwol, T ^a   Merten, E ^a   Opitz, N ^a   Acker, H ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

Author keywords

Cell volume; Confocal laser scanning microscopy; Electromagnetic field strength; Human melanoma cells; Image processing; Mitochondrial membrane potential; Multicellular spheroids; Optical sectioning; Rhodamine 123; Three dimensional imaging

Indexed keywords

RHODAMINE 123;

ARTICLE; CANCER CELL; CONFOCAL LASER MICROSCOPY; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; LASER MICROSCOPY; MELANOMA; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

FLUORESCENT DYES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MELANOMA; MICROSCOPY, CONFOCAL; RHODAMINE 123; RHODAMINES; TUMOR CELLS, CULTURED;

EID: 0030441978     PISSN: 14226405     EISSN: 14226421     Source Type: Journal    
DOI: 10.1159/000147872     Document Type: Article

References (29)

1. Argvle, E. (1971) Techniques lor edge detection. Proc IEEE59;285-287.
2. Ashkar, G.P., J.W. Modesting (1978) The contour extraction problem with biomedical application. Comput Graphics Image Processing 7; 331-355.
3. Carlsson, K. (1991) The influence of specimen refractive index, detector signal integration, and nonuniform scan speed on the imaging properties in confocal microscopy. J Microsc 163; 167-178.
4. Duchen, M.R., T.J. Biscoe (1992) Relative mitochondrial membrane potential and (Ca‘), in type I cells isolated from the rabbit carotid body. J Physiol 450;33-61.
5. Emaus, R.K., R. Grunwald, J.I. Lemasters (1986) Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria; Spectral and metabolic properties. Biochim Biophys Acta 850;436-448.
6. Gorlach, A., B. Bolling, G. Holtermann, J. Schwaehofcr, J. Carlsson, H. Acker (1995) Changes in growth. PO, and pH after exposure to oxamate; Studies of two human tumour cell lines growing as multicellular spheroids. Int J Oncol 7;831-839.
7. Gorlach, A., P Herter, H. Hentschel, P.J. Frosch, H. Acker (1994) Effects of nlFN P and rIFN yon growth and morphology of two human melanoma cell lines; Comparison between two-and three-dimensional culture. Int J Cancer 56; 249-254.
8. Hell, S., E.H.K. Stelzcr (1993) Aberrations in con-focal fluorescence microscopy induced by mismatches in refractive index. J Microsc 169; 391-495.
9. Hell, S.W., E.H.K. Stelzer (1995) Lens aberrations in confocal fluorescence microscopy; in Pawley JB (ed); Handbook of Biological Confocal Microscopy, ed 2. New York, Plenum, pp 347-354.
10. Huang, T.S., G.J. Yang, G.Y. Tang (1979) A fast two-dimensional median filtering algorithm. IEEE Trans Acoust Speech Signal Processing ASSP-27; 13-18.
11. Johnson, V.L., M.L. Walsh, L.B. Chen (1986) Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sei USA 77; 990-994.
12. Lorensen, W.E., H.E. Cline (1987) Marching Cubes; A high resolution 3D surface construction algorithm. Comput Graphics 21; 163-169.
13. Majlof, I, P.O. Forsgren (1993) Confocal microscopy; Important considerations for accurate imaging. Methods Cell Biol 38; 79-95.
14. Opitz, N., E. Merten, H. Acker (1994) Evidence for redistribution-associated intracellular pK shifts of the pi 1-sensilive fluoroprobe carboxy-SNARF-1. Pflügers Arch 427; 332-342.
15. Opitz, N., T. Porwol, E. Merten, H. Acker (1996) On the protein-error of the calcium-sensitive fluorescent indicator fura-red. In Slavik.1 (ed); Fluorescence Microscopy and Fluorescent Probes. New York. Plenum.
16. Parker, J.R. (1994) Practical Computer Vision Using C. New York. Wiley & Sons.
17. Schnablegger, H., O. Glatter (1993) Simultaneous determination of size distribution and refractive index of colloidal particles from static light scattering experiments. J Colloid Interface Sei 158;228-242.
18. Schweiger, G. (1994) Optische Resonatoren aus feinsten Tröpfchen. Spektrum Wiss Vol II; 110-118.
19. Sheehan, B., S.D. Fuller, M.E. Pique, M. Yeager (1996) AVS software visualization in molecular microscopy. J Struct Biol 116;99-106.
20. Shotton, D.M. (1995) Electronic light microscopy; Present capabilities and future prospects. His-tochem Cell Biol 104; 97-137.
21. Slrasters, K.C. (1994) Quantitative Analysis in Confocal Image Cytometry; PhD thesis Delft University.
22. Thalmann, N., D. Thalmann (1991) New Trends in Animation and Visualization. Chichester. Wiley & Sons.
23. Tsien, R.Y., M. Poenie (1986) Fluorescence ratio imaging; A new window into intracellular ionic signaling. Trends Biochem Sci Vol II; 450-455.
24. Van der Voort, H.T.M. (1989) Three-Dimensional Image Formation and Processing in Confocal Microscopy; PhD thesis University of Amsterdam.
25. Visser, T.D. (1992) Diffraction of Electromagnetic Waves and Image Formation in Confocal Microscopy; PhD thesis University of Amsterdam.
26. Visscr, T.D., J.L. Oud, G.J. Brakenhoff (1992) Refractive index and distance measurements in 3-D microscopy. Oplik 90; 17-19.
27. Weast, R.C. (1983) CRC Handbook of Chemistry and Physics, ed 64. Boca Raton. CRC Press.
28. Wilhelms, J., A. Van Gelder (1990) Topological considerations in isosurface generation. Comput Graphics 24; 79-86.
29. Wright, S.J., V.E. Centonze, S.A. Strieker, P.J. DeVries, S.W. Paddock, G. Schatten (1993) Introduction to confocal microscopy and three-dimensional reconstruction. Methods Cell Biol 38; 1-45.