Mitosis-specific phosphorylation of smooth muscle regulatory light chain of myosin II at Ser-1 and/or -2 and Thr-9 in sea urchin egg extract

Cell Structure and Function

Volumn 21, Issue 6, 1996, Pages 475-482

  Totsukawa, Go ^a   Himi Nakamura, Eiko ^a   Komatsu, Satoshi ^a   Iwata, Kyoko ^b,c   Tezuka, Ayako ^b,c   Sakai, Hikoichi ^b   Yazaki, Kazumori ^c   Hosoya, Hiroshi ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b JAPAN WOMEN'S UNIVERSITY   (Japan)

^c TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

Author keywords

Cdc2 kinase; Cell cycle; MLCK; Myosin regulatory light chain; Phosphorylation; Sea urchin

Indexed keywords

HISTONE H1; MYOSIN LIGHT CHAIN; MYOSIN LIGHT CHAIN KINASE; PHOSPHOAMINO ACID; PHOSPHOPEPTIDE; PROTEIN KINASE C; SERINE; THREONINE;

ARTICLE; AVIAN STOMACH; CELL CYCLE; CELL DIVISION; CHICKEN; METAPHASE; MITOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SEA URCHIN;

AVES; ECHINOIDEA; GALLUS GALLUS;

EID: 0030441292     PISSN: 03867196     EISSN: None     Source Type: Journal    
DOI: 10.1247/csf.21.475     Document Type: Article

References (32)

1. ADELSTEIN, R.S. and KLEE, C.B. 1981. Purification and characterization of smooth muscle myosin light chain kinase. J. Biol. Chem., 256: 7501-7509.
2. BENGUR, A.R., ROBINSON, E.A., APPELLA, E., and SELLERS, J.R. 1987. Sequence of the sites phosphorylated by protein kinase C in the smooth muscle myosin light chain. J. Biol. Chem., 262: 7613-7617.
3. BLATTER, D.P., GARNER, F., VAN SLYKE, K., and BRADLEY, A. 1972. Quantitative electrophoresis in polyacrylamide gels of 2-40%. J. Chromatogr., 64: 147-155.
4. BRADFORD, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of ptotein-dye binding. Anal. Biochem., 72: 248-254.
5. 2+-calmodulin-dependent kinase with myosin light chain phosphorylating activity. J. Biol. Chem., 261: 5389-5395.
6. DELOZANNE, A. and SPUDICH, J.A. 1987. Disruption of the Dictyosterium myosin heavy-chain gene by homologous recombination. Science, 236: 1086-1091.
7. FUJIWARA, K. and POLLARD, T.D. 1976. Fluorescent antibody localization of myosin in the cytoplasm, claevage furrow, and mitotic spindle of human cells. J. Cell Biol., 71: 848-875.
8. HOSOYA, H., YAMASHIRO, S., and MATSUMURA, F. 1991. Mitosis-phosphorylation of myosin light chain kinase. J. Biol. Chem., 266: 22173-22178.
9. HOSOYA, H. 1994. Cell-cycle-dependent regulation of myosin light chain kinase. Zool. Sci., 11: 507-510.
10. HOSOYA, N., HOSOYA, H., MOHRI, T., and MOHRI, H. 1990. A 70 kD microtuble-binding protein from starfish eggs: purification, characterization, and localization during meiosis and mitosis. Cell Motil. Cytoskel., 15: 168-180.
11. HUNTER, T. and SEFTON, B.M. 1980. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc. Natl. Acad. Sci. USA, 77: 1311-1315.
12. 2+ on the conformation and enzymatic activity of smooth muscle myosin. J. Biol. Chem., 260: 13146-13153.
13. IKEBE, M., HARTSHORNE, D.J., and ELZINGA, M. 1987. Phosphorylation of the 20,000-dalton light chain of smooth muscle myosin by the calcium-activated, phospholipid-dependent protein kinase. J. Biol. Chem., 262: 9569-9573.
14. KAWAMOTO, S.A. and ADELSTEIN, R.S. 1987. Characterization of myosin heavy chains in cultured aorta smooth muscle cells. J. Biol. Chem., 262: 7282-7288.
15. KITAGAWA, M., OKABE, T., OGINO, H., MATSUMOTO, H., SUZUKI-TAKAHASHI, I., KOKUBO, T., HIGASHI, H., SAITOH, S., TAYA, Y., YASUDA, H., OHBA, Y., NISHIMURA, S., TANAKA, N., and OKUYAMA, A. 1993. Butyrolactone I, a selective inhibitor of cdk2 and cdc2 kinase. Oncogene, 8: 2425-2432.
16. KNECHT, D. and LOOMIS, W.F. 1987. Antisence RNA inactivation of myosin heavy-chain gene-expression in Dictyosterium discoideum. Science, 236: 1081-1086.
17. 2+-ATPase activity of myosin II. Biochem. Biophys. Res. Commun., 223: 741-745.
18. LAEMMLI, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
19. MABUCHI, I. and OKUNO, M. 1977. The effect of myosin antibody on the division of starfish blastmeres. J. Cell Biol., 74: 251-263.
20. MABUCHI, I. 1986. Biochemical aspects of cytokinesis. Int. Rev. Cytol., 101: 175-213.
21. MISHIMA, M. and MABUCHI, I. 1996. Cell cycle-dependent phosphorylation of smooth muscle myosin light chain in sea urchin egg extracts. J. Biochem., 119: 906-913.
22. NISHIKAWA, M., SELLERS, J.R., ADELSTEIN, R.S., and HIDAKA, H. 1984. Protein kinase C modulates in vitro phosphorylation of the smooth muscle meromyosin by myosin light chain kinase. J. Biol. Chem., 259: 8808-8814.
23. PERRIE, W.T. and PERRY, S.V. 1970. An electrophoretic study of low-molecular-weight components of myosin. Biochem. J., 119: 31-38.
24. RAPPAPORT, R. 1986. Establishment of the mechanism of cytokinesis in animal cells. Int. Rev. Cytol., 101: 245-281.
25. SANGER, J.M., MITTAL, B., DOME, J.S., and SANGER, J.W. 1989. Analysis of cell division using fluorescent labeled actin and myosin in living PtK2 cells. Cell Motil. Cytoskel., 14: 201-219.
26. cdc2. a mechanism for the timing of cytokinesis. J. Cell Biol., 118: 595-605.
27. SATTERWHITE, L.L. and POLLARD, T.D. 1992. Cytokinesis. Curr. Opin. Cell Biol., 4: 43-52.
28. SCHROEDER, T.E. 1976. Actin in dividing cells: evidence for its roles in cleavage but not mitosis. In Cell Motility (Goldman, R., Pollard, T., and Rosenbaum, J., eds.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New Yok, pp.265-278.
29. SELLERS, J.R. 1990. Regulation of cytoplasmic and smooth muscle myosin. Curr. Opin. Cell Biol., 3: 98-104.
30. TAN, J.L. and SPUDICH, J.A. 1990. Dictyosterium myosin light chain kinase. J. Biol. Chem., 265: 13818-13824.
31. YAMAKITA, Y., YAMASHIRO, S., and MATSUMURA, F. 1994. In vivo phosphorylation of regulatory light chain of myosin II during mitosis of cultured cells. J. Cell Biol., 124: 129-137.
32. YOSHIGAKI, T., MAEKAWA, S., ENDO, S., and SAKAI, H. 1989. 51-kd protein, a component of microtubule-organizing granules in the mitotic apparatus involved in aster formation in vitro. Cell Struct. Funct., 14: 363-374.