Degradative activities in a recombinant Chinese hamster ovary cell culture

Cytotechnology

Volumn 22, Issue 1-3, 1996, Pages 43-52

  Lao, Mio Sam ^a,b   Toth, Derek ^a   Danell, Glenys ^a   Schalla, Calvin ^a  

^a Cangene Corporation   (Canada)

^b NONE   (United States)

Author keywords

bioreactor; glycosidase; insulin degradative activity; recombinant CHO cells

Indexed keywords

CRICETINAE; CRICETULUS GRISEUS;

EID: 0030432048     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00353923     Document Type: Conference Paper

References (13)

1. Ali M and Plas C (1989) Glucocorticoid regulation of chloroguine nonsensitive insulin degradation in cultured fetal rat hepatocytes. J. Biol. Chem. 264 (35): 20992-7.
2. Drapeau D, Luan Y-T, Popoloski J, Richards D, Cohen D, Sinacore M and Adamson S (1994) Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process. Cytotechnology 15: 103-109.
3. Gramer M, Goochee C (1993) Glycosidase activities in Chinese hamster ovary cells lysate and cell culture supernatant. Biotechnol. Prog. 9: 366-373.
4. Gramer M, Goochee C, Chock V, Brousseau D and Sliwkowski M (1995) Removal of sialic acid from a glycoprotein in CHO cell culture supernatant by action of an extracellular CHO cell sialidase. Biotechnology 13: 692-698.
5. Kooistra T and Lloyd JB (1985) Degradation of insulin by human fibroblasts: effects of inhibitors of pinocytosis and lysosomal activity. Int. J. Biochem. 17 (7): 805-811.
6. Lund P (1985) L-glutamine and L-glutamate. In: Bergmeyer HU (ed.) Methods of enzymatic analysis. Vol. 8: 357-363. VCH Weinheim.
7. Minch SL, Kallio PT and Bailey JE (1995) Tissue plasminogen activator coexpressed in Chinese hamster ovary cells with α(2,6)-sialytransferase contains neuAc α(2,6)galβ(1,4)glc-N-AcR linkages. Biotechnol. Prog. 11: 348-351.
8. Omasa T, Higashiyama K, Shioya S and Suga K (1992) Effects of lactate concentration on hybridoma culture in lactate-controlled fed-batch operation. Biotech. Bioeng. 39: 556-564.
9. Renner WA, Lee KH, Hatzimanikatis V, Baily JE and Eppenberger HM (1995) Recombinant cyclin E expression activates proliferation and obviates surface attachment of Chinese hamster ovary (CHO) cells in protein-free medium. Biotech. Bioeng. 47: 476-482.
10. Schumpp B and Schlaeger E-J (1992) Growth study of lactate and ammonia double-resistant clones of HL-60 cells. In: Spier RE, Griffiths JB and MacDonald C (eds.) Animal cell technology: developments, processes and products (pp. 183-185). Butterworth-Heinemann.
11. Takahashi K, Tereda S, Ueda H, Makishima F and Suzuki E (1994) Growth rate suppression of cultured mammalian cells enhances protein productivity. Cytotechnology 15: 57-64.
12. Weidemann R, Ludwig A and Kretzmer G (1994) Low temperature cultivation - a step towards process optimisation. Cytotechnology 15: 111-116.
13. Xie L and Wang DIC (1994) Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor. Cytotechnology 15: 17-29.