Lysis-free separation of hybridoma cells by continuous disc stack centrifugation

Cytotechnology

Volumn 22, Issue 1-3, 1996, Pages 119-127

  Tebbe, H ^a   Lutkemeyer D ^a   Gudermann, F ^a   Heidemann, R ^a   Lehmann, J ^a  

^a BIELEFELD UNIVERSITY   (Germany)

Author keywords

cell separation; continuous centrifugation; hybridoma cells; product recovery

Indexed keywords

MAMMALIA;

EID: 0030432003     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00353931     Document Type: Conference Paper

References (16)

1. Abu-Reesh I and Kargi F (1989) Biological responses of hybridoma cells to defined hydrodynamic shear stress. J Biotechnology 9: 167-178.
2. Berthold W and Kempken R (1994) Interaction of cell culture with downstream purification: a case study. Cytotechnology 15: 229-242.
3. Büntemeyer H, Lütkemeyer D and Lehmann J (1991) Optimization of serum-free fermentation processes for antibody production. Cytotechnology 5: 57-67.
4. Draeger NM and Chase HA (1991) Liquid fluidized bed adsorption of protein in the presence of cells. Bioseparation 2: 67-80.
5. Glauner B (1991) Vitalitätskontrolle in der Zellkulturtechnik. BioTec 3(5): 58-61.
6. Gudermann F, Lütkemeyer D and Lehmann J (1994) Design of a dubble-swarm bioreactor for animal cell culture. Cytotechnology 15: 301-309.
7. Hemfort H (1983) Separators. Technical scientific documentation No. 1, booklet No. 9997-7133-010/0483, Westfalia Separator AG, Oelde, Germany.
8. Jäger V, Lehmann J and Friedel P (1988) Serum free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors. Cytotechnology 1: 319-329.
9. Kempken R, Preissmann A and Berthold W (1995) Clarification of animal cell cultures on a large scale by continuous centrifugation. J Indust Microbiol 14: 52-57.
10. Kempken R, Preissmann A and Berthold W (1995) Assessment of a disc stack centriguge for use in mammalian cell separation. Biotechnol Bioeng 46: 132-138.
11. Kuhlmann W (1987) Optimization of a membrane oxygenation system for cell culture in stirred tank reactors. Dev Biol Standard 66: 263-268.
12. Le MS and Gollan KL (1989) Fouling of microporous membranes in biological applications. J Membrane Science 40: 231-242.
13. Lehmann J, Piehl GW and Schulz R (1987) Bubble free cell culture aeration with porous moving membranes. Dev Biol Standard 66: 227-240.
14. Mannweiler K and Hoare M (1992) The scale down of an industrial disc stack centrifuge. Bioprocess Engineering 8: 19-25.
15. Meireles M (1991) Effects of protein fouling on the apparent pore size distribution of sieving membranes. J Membrane Science 56: 231-242.
16. Rajiv VD and Rosen CG (1993) Cell and cell debris removal: Centrifugation and crossflow filtration. In: Rehm et al. (eds) Biotechnology. Vol. 3, pp 469-487.