Super-CHO - A cell line capable of autocrine growth under fully defined protein-free conditions

Cytotechnology

Volumn 22, Issue 1-3, 1996, Pages 139-146

  Pak, S C O ^a   Hunt, S M N ^a   Bridges, M W ^a   Sleigh, M J ^b,c   Gray, P P ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b CSIRO   (Australia)

^c Peptide Technology Ltd   (Australia)

Author keywords

autocrine growth; CHO; IGF 1; insulin; transferrin

Indexed keywords

CRICETINAE; CRICETULUS GRISEUS;

EID: 0030431008     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00353933     Document Type: Conference Paper

References (16)

1. Butler M (1986) Serum-free media. In: Thilly WG (ed.) Mammalian Cell Technology. (pp. 91-108) Butterworths, Boston.
2. Chen C and Okayama H (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biochem 7: 2745-2752.
3. Dai Z, Takahashi S-I, van Wyk JJ and D'ercole AJ (1992) Creation of an autocrine model of insulin-like growth factor-I action in transfected FRTL-5 cells. Endocrinology 130: 3175-3183.
4. Evans VJ, Bryant JC, Fioramonti MC, McQuilkin WT, Standford KK and Earle WR (1956) Studies of nutrient media for tissue C cells in vitro. A protein-free chemically defined medium for cultivation of strain L cells. Cancer Res 16: 77-86.
5. Gasser F, Mulsant P and Gillois M (1985) Long-term multiplication of the Chinese Hamster Ovary (CHO) cell line in serum-free medium. In Vitro cell Devel Biol 21: 588-592.
6. Groskrentz DJ, Sliwkowski MX and Gorman CM (1994) Genetically engineered proinsulin constitutively processed and secreted as mature, active insulin. J Biol Chem 269: 6241-6245.
7. Hamilton WG and Ham RG (1977) Clonal growth of Chinese Hamster cell lines in protein-free media. In Vitro 13: 537-547.
8. Hunt SMN, Pak SCO, bridges M, Gray PP, Sleigh MJ (submitted for publication).
9. Mendiaz E, Mamounas M, Moffett J and Engleberg E (1986) A defined medium for and the effect of insulin on the growth, amino acid transport, and morphology of Chines Hamster Ovary cell, CHO-K1 (CCL 61) and the isolation of insulin 'independent' mutants. In Vitro Cell Devel Biol 22: 66-74.
10. Pietrzkowski Z, Lammers R, Carpenter G, Soderquist AM, Limardo M, Phillips PD, Ullrich A and Baserga R (1992) Constitutive expression of insulin-like growth factor-I and insulin-like growth factor-I receptor abrogates all requirement for exogenous growth factors. Cell Growth and Differentiation 3: 199-205.
11. Podlecki DA, Frank BH and Olefsky JM (1984) In Vitro characterisation of biosynthetic human proinsulin. Diabetes 33: 111-116.
12. Quin D, Orci L, Ravazzola M and Moore H-PH (1991) Intracellular transport and sorting of mutant human proinsulins that fail to form hexamers J Cell Biol 113: 987-996.
13. Sambrook J, Fritsch EF and Maniatis T (1989) Detection and analysis of proteins expressed from cloned genes. In: Irwin N, Ford N, Nolan C and Fergusin M (ed) Molecular cloning. A laboratory manual, 2nd edition, (vol 3, p 18.74). Gold Springs Harbor Laboratory Press, USA.
14. Vollenweider F, Irminger J-C, Cross DJ, Villa-Komaroff L and Halban PA (1992) Processing of proinsulin by transfected Hepatoma (FAO) cells. J Biol Chem 267: 14629-14636.
15. Watanabe T, Nakagawa T, Ikemizu J, Nagahama M, Murakami K and Nakayama K (1992) Sequence requirements for precursor cleavage within the constitutive secretory pathway. J Biol Chem 267: 8270-8274.
16. Yanagita M, Nakayama K and Takeuchi T (1992) Processing of mutated proinsulin with tetrabasic cleavage sites to bioactive insulin in the non-endocrine cell line, COS-7. FEBS Letters 311: 55-59.