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2+ and p-nitrophenol hydrolyzed from p-nitrophenyl dihydrogenphosphate.
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SDS-PAGE was run in a gel containing 12.5% (w/v) polyacrylamide and 0.1% (w/v) SDS. Prior to electrophoresis, the sample protein was heated at 90 °C for 30 min in 0.01 M Tris-HCl buffer soin (pH 6.8) containing 0.2% (w/v) of SDS, 1 % (w/v) of 2-mercaptoethanol, and 2% (w/v) of glycerol.
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MALDI-TOF mass spectrum of the sample protein in 10 mM Tris-HCl buffer was recorded on a JMS-LDI 1700 instrument at MULDI(+) ionization mode (Repeller 30 kV and Extractor 10.5 kV) using sinapinic acid as matrix.
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