In vitro expression of a mouse tissue specific glutathione-peroxidase-like protein lacking the selenocysteine can protect stably transfected mammalian cells against oxidative damage

Biochemistry and Cell Biology

Volumn 74, Issue 1, 1996, Pages 125-131

  Vernet, Patrick ^a   Rigaudiére, Nicole ^a   Ghyselinck, Norbert ^a,b   Dufaure, Jean Pierre ^a   Drevet, Joël R ^a  

^a CNRS   (France)

^b INSTITUT DE GÉNÉTIQUE ET DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

Author keywords

CHO KI cells; Glutathione peroxidase (GPX); GPX5 cDNA; GPX5 polyclonal antibody; MEP24; Mouse epididymis; Selenocysteine; Spermatozoa

Indexed keywords

GLUTATHIONE PEROXIDASE; GPX5 PROTEIN, MOUSE; PEROXIDE; SELENIUM; SELENIUM INDEPENDENT GLUTATHIONE PEROXIDASE; SELENIUM-INDEPENDENT GLUTATHIONE PEROXIDASE; TESTIS PEPTIDE HORMONE;

AMINO ACID SEQUENCE; ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; CHEMISTRY; CHO CELL; ENZYMOLOGY; EPIDIDYMIS; GENETIC TRANSFECTION; GENETICS; HAMSTER; MALE; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; EPIDIDYMIS; GLUTATHIONE PEROXIDASE; MALE; MICE; MOLECULAR SEQUENCE DATA; ORGAN SPECIFICITY; OXIDATIVE STRESS; PEROXIDES; SELENIUM RADIOISOTOPES; TESTICULAR HORMONES; TRANSFECTION;

EID: 0030348185     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o96-014     Document Type: Article

References (5)

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2. Alvarez, J.G., and Storey, B.T. 1984. Lipid peroxidation and the reactions of Superoxide and hydrogen peroxide in mouse spermatozoa. Biol. Reprod. 30: 833-841.
3. Avissar, N., Eisemann, C., Breen, J.G., Horowitz, S., Miller, R.K., and Cohen, H.J. 1994. Human placenta makes extracellular glutathione peroxidase and secretes it into maternal circulation. Am. J. Physiol. 267: 68-76.
4. Chambers, I., Frampton, J., Goldfarb, P., Affara, N., McBain, W., and Harrison, P.R. 1986. The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the termination codon, TGA. EMBO J. 5: 1221-1227.
5. Chaudière, J., and Gérard, D. 1988. Dosage de l'activité glutathion peroxydase. In Biologie des lipides chez l'homme. Edited by L. Douste-Blazy and F. Mendy. Editions médicales internationales, Paris. pp.275-279.