Spontaneous and Ethylnitrosourea-Induced Mutation Fixation and Molecular Spectra at the lacl Transgene in the Big Blue® Rat-2 Embryo Cell Line

Environmental and Molecular Mutagenesis

Volumn 28, Issue 4, 1996, Pages 325-333

  Zimmer, D M ^a   Zhang, X B ^a   Harbach, P R ^a   Mayo, J K ^a   Aaron, C S ^a  

^a PFIZER INC   (United States)

Author keywords

ENU induced mutation spectra; lacl transgene; Mutagenesis; Spontaneous mutation spectra

Indexed keywords

BACTERIAL PROTEIN; ESCHERICHIA COLI PROTEIN; ETHYLNITROSOUREA; LACI PROTEIN, BACTERIA; MUTAGENIC AGENT; REPRESSOR PROTEIN;

ANIMAL; ARTICLE; CELL DEATH; CELL DIVISION; CELL LINE; CYTOLOGY; DNA SEQUENCE; DOSE RESPONSE; DRUG EFFECT; EMBRYO; GENETICS; METHODOLOGY; MUTAGEN TESTING; MUTATION; PHYSIOLOGY; RAT; TRANSGENE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BACTERIAL PROTEINS; CELL DEATH; CELL DIVISION; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; EMBRYO; ESCHERICHIA COLI PROTEINS; ETHYLNITROSOUREA; MUTAGENICITY TESTS; MUTAGENS; MUTATION; RATS; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA; TRANSGENES;

EID: 0030344383     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1098-2280(1996)28:4<325::aid-em5>3.0.co;2-a     Document Type: Article

References (33)

1. Burns PA, Gordon AJE, Kumsmann K, Glickman BW (1988): Influence of neighboring hase sequence on the distribution and repair of N-ethyl-N-nitrosourea-induced lesions in Escherichia coli. Cancer Res 48:4455-4458.
2. Dycaico MJ, Provost GS, Kretz PL, Ransom SL, Moores JC, Short JM (1994): The use of shuttle vectors for mutation analysis in transgenic mice and rat. Mutat Res 307:461-478.
3. Eckert KA, Ingle CA, Klinedinst DK, Drinkwater NR (1988): Molecular analysis of mutations induced in human cells by N-ethyl-N-nitrosourea. Mol Carcinogen 1:50-56.
4. Garberg P, Akerblom E-L, Bolcsfoldi G (1988): Evaluation of a genotoxicity test measuring DNA-strand breaks in mouse lymphoma cells by alkaline unwinding and hydroxyapatite elution. Mutat Res 203:155-176.
5. Gorelick NJ, Andrews JL, Gu M, Glickman BW (1995): Mutational spectra in the lacl gene in skin from 7,12-dimethylbenz[a]-anthracene-treated and untreated transgenic mice. Mol Carcinogen 14:53-62.
6. Guttenplan JB (1990): Mutagenesis by N-nitroso compounds: Relationship to DNA adducts, DNA repair, and mutational efficiencies. Mutat Res 233:177-187.
7. Guttenplan JB, Hu Y-C (1984): Mutagenesis by N-nitroso compounds in TA102 and TA104 Salmonella typhimurium: Evidence for premutagenic adenine or thymine DNA adduct. Mutat Res 141:153-159.
8. Harbach PR, Filipunas AL, Wang Y, Aaron CS (1992): DNA sequence analysis of spontaneous and N-ethyl-N-nitrosourea-induced hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes. Environ Mol Mutagen 20:96-105.
9. Harbach PR, Mattano SS, Zimmer DM, Wang Y, Aaron CS (1995): DNA sequencing of spontaneous hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes. Environ Mol Mutagen 26:218-225.
10. Heddle JA, Shaver-Walker PS, Tao KS, Zhang XB (1995): Treatment protocols for transgenic mutation assays in vivo. Mutagenesis 6:467-470.
11. Kohler SW, Provost GS, Kretz PL, Fieck A, Sorge JA, Short JM (1990a): Development of a short-term, in vivo mutagenesis assay: The effects of methylation on the recovery of a lambda phage shuttle vector from transgenic mice. Nucleic Acids Res 18:3007-3013.
12. Kohler SW, Provost GS, Kretz PL, Fieck JA, Sorge JA, Short JM (1990b): The use of transgenic mice for short-term, in vivo mutagenicity testing. Genet Anal Tech Appl 7:212-218.
13. Kohler SW, Provost GS, Fieck JA, Kretz PL, Sorge JA, Bullock JA, Short JM (1991): Spectra of spontaneous and mutagen-induced mutations in the lacl gene in transgenic mice. Proc Natl Acad Sci USA 88:7958-7962.
14. Lewis SE, Johnson FM, Skow LC, Popp D, Barnett LB, Popp RA (1985): A mutation in the β-globin gene detected in the progeny of a female mouse treated with ethylnitrosourea. Proc Natl Acad Sci USA 82:5829-5831.
15. Mirsalis JC, Provost GS, Matthews CD, Hamner RT, Schindler JE, O'Loughlin KG, MacGregor JT, Short JM (1993): Induction of hepatic mutation in lacl transgenic mice. Mutagenesis 8:265-271.
16. Paashuis-Lew Y, Zhang XB, Heddle JA (1996): Ex vivo mutants, expression time, and the Big Blue™ lacl transgenic mouse. Environ Mol Mutagen 27(Suppl 27):53.
17. Pastink AC, Vreeken MJM, Nivard LL, Searles AA, Vogel EW (1989): Sequence analysis of N-ethyl-N-nitrosourea-induced vermilion mutations in Drosophila melanogaster. Genetics 123:123-129.
18. Popp RA, Bailiff EG, Skow LC, Johnson FM, Lewis SE (1983): Analysis of a mouse alpha-globin gene mutation induced by N-ethyl-N-nitrosourea. Genetics 105:157-167.
19. Provost GS, Short JM (1994): Characterization of mutations induced by ethylnitrosourea in seminiferous tubule germ cells of transgenic B6C3F1 mice. Proc Natl Acad Sci USA 91:6564-6568.
20. Provost GS, Kretz PL, Hamner RT, Matthews CD, Rogers BJ, Lundberg KS, Dycaico MJ, Short JM (1983): Transgenic systems for in vivo mutation analysis. Mutat Res 288:133-149.
21. Richardson KK, Richardson FC, Crosby RM, Swenberg JA, Skopek TR (1987): DNA base change and alkylation following in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea. Proc Natl Acad Sci USA 84:344-348.
22. Sands AT, Suraokar MS, Sanchez A, Marth JE, Donehower LA, Bradley A (1995): p53 deficiency does not affect the accumulation of point mutations in a transgene target. Proc Natl Acad Sci USA 92:8517-8521.
23. Schaaper RM, Danforth BN, Glickman BW (1986): Mechanisms of spontaneous mutagenesis: An analysis of the spectrum of spontaneous mutation in the Escherichia coli lacl gene. J Mol Biol 189:273-284.
24. Shibuya T, Morimoto K (1993): A review of the genotoxicity of 1-ethyl-1-nitrosourea. Mutat Res 297:3-38.
25. 1 mice. Environ Mol Mutagen 26:9-15.
26. Streisinger G, Okada Y, Newton J, Tsugita A, Terzaghi E, Inouye M (1966): Frameshift mutations and the genetic code. Cold Spring Harb Symp Quant Biol 31:77-84.
27. Tao KS, Urlando C, Heddle JA (1993): Comparison of somatic mutation in a transgenic versus host locus. Proc Natl Acad Sci USA 90:10681-10685.
28. Vrieling H, Niericker MJ, Simons JWIM, Van Zeeland AA (1988): Molecular analysis of mutations induced by N-ethyl-N-nitrosourea at the HPRT locus in mouse lymphoma cells. Mutat Res 198:99-106.
29. Walker VE, Clement N, Andrews JL, Upton PB, Sussman HE, Gorelick NJ (1996): Spectrum of cyclophosphamide induced mutation in hprt exon 3 of splenic T-cells from exposed lacl transgenic and nontransgenic B6C3F1 mice. Environ Mol Mutagen 27(Suppl 27):72.
30. Winegar RA, Lutze LH, Hamer JD, O'Loughlin KG, Mirsalis JC (1994): Radiation-induced point mutations, deletions and micronuclei in lacl transgenic mice. Mutat Res 307:479-487.
31. Wyborski DL, Malkhosyan S, Moores J, Perucho M, Short JM (1995): Development of a rat cell line containing stably integrated copies of a lambda/lacl shuttle vector. Mutat Res 334:161-165.
32. Zhang XB, Urlando C, Tao KS, Heddle JA (1995): Factors affecting somatic mutation frequencies in vivo. Mutat Res 338:189-201.
33. Zhang XB, Tao KS, Urlando C, Shaver-Walker P, Heddle JA (1996): Mutagenicity of high fat diets in the colon and small intestine of transgenic mice. Mutagenesis 11:43-48.