Development of a Novel Mouse tk+/- Embryonic Stem Cell Line for Use in Mutagenicity Studies

Environmental and Molecular Mutagenesis

Volumn 28, Issue 4, 1996, Pages 483-489

  Dobrovolsky, Vasily N ^a   Casciano, Daniel A ^a   Heflich, Robert H ^a  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

Embryonic stem cells; Homologous recombination; Mutation; N ethyl N nitrosourea; Rhymidine kinase

Indexed keywords

MUS MUSCULUS;

ANTIMETABOLITE; ETHYLNITROSOUREA; MUTAGENIC AGENT; THYMIDINE KINASE; TRIFLURIDINE;

ANIMAL; ARTICLE; CELL LINE; CYTOLOGY; DRUG EFFECT; DRUG RESISTANCE; EMBRYO; GENE VECTOR; GENETIC RECOMBINATION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOUSE; MUTAGEN TESTING; MUTATION; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; STEM CELL;

ANIMALS; ANTIMETABOLITES; BLOTTING, SOUTHERN; CELL LINE; CLONING, MOLECULAR; DRUG RESISTANCE; EMBRYO; ETHYLNITROSOUREA; GENETIC VECTORS; MICE; MUTAGENICITY TESTS; MUTAGENS; MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; STEM CELLS; THYMIDINE KINASE; TRIFLURIDINE;

EID: 0030337541     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1098-2280(1996)28:4<483::aid-em26>3.0.co;2-a     Document Type: Article

References (45)

1. Adair GM, Carver JH, Wandres DL (1980): Mutagenicity testing in mammalian cells. I. Derivation of a Chinese hamster ovary cell line heterozygous for the adenine phosphoribosyltransferase and thymidine kinase loci. Mutat Res 72:187-205.
2. Andrews JL, Walker VE, Upton PB, deBoer JG, Gorelick NJ (1996): Cyclophosphamide-induced mutations are detected at the hprt but not the lacl loci in splenic T-cells of exposed Big Blue® mice. Environ Mol Mutagen 27(Suppl 27):4.
3. Applegate ML, Moore MM, Broder CB, Burrell A, Juhn G, Kasweck KL, Lin P-F, Wadhams A, Hozier JC (1990): Molecular dissection of mutations at the heterozygous thymidine kinase locus in mouse lymphoma cells. Proc Natl Acad Sci USA 87:51-55.
4. Berk AJ, Clayton DA (1973): A genetically distinct thymidine kinase in mammalian mitochondria. J Biol Chem 248:2722-2729.
5. Bradley WE, Letovanec D (1982): High-frequency nonrandom mutational event at the adenine phosphoribosyltransferase (aprt) locus of sib-selected CHO variants heterozygous for aprt. Somat Cell Genet 8:51-66.
6. Burkhart JG, Burkhart BA, Sampson KS, Malling HV (1993): ENU-induced mutagenesis at a single A:T base pair in transgenic mice containing ΦX174. Mutat Res 292:69-81.
7. Chu E, Malling HV (1968): Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc Natl Acad Sci USA 61:1306-1312.
8. Clive D, Spector J-AFS (1975): Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells. Mutat Res 31:17-29.
9. Clive D, Flamm WG, Machesko MR, Bernheim NJ (1972): A mutational assay system using the thymidine kinase locus in mouse lymphoma cells. Mutat Res 16:77-87.
10. +/- mouse lymphoma mutagen assay system. Mutat Res 59:61-108.
11. +/- mouse lymphoma cells. Mutagenesis 5:191-197.
12. DeMarini DM, Brockman HE, de Serres FJ, Evans HH, Stankowski LF Jr, Hsie AW (1989): Specific-locus mutations induced in eukaryotes (especially mammalian cells) by radiation and chemicals: A perspective. Mutat Res 220:11-29.
13. Dobrovic A, Gareau JL, Ouellette G, Bradley WE (1988): DNA methylation and genetic inactivation at thymidine kinase locus: Two different mechanisms for silencing autosomal genes. Somat Cell Mol Genet 14:55-68.
14. Dycaico MJ, Provost GS, Kretz PL, Ransom SL, Moores JC, Short JM (1994): The use of shuttle vectors for mutation analysis in transgenic mice and rats. Mutat Res 307:461-478.
15. Engle SJ, Stockelman MG, Chen J, Boivin G, Yum M-N, Davies PM, Ying MY, Sahota A, Simmonds HA, Stambrook PJ, Tischfield JA (1996): Adenine phosphoribosyltransferase-defident mice develop 2,8-dihydroxyadenine nephrolithiasis. Proc Natl Acad Sci USA 93:5307-5312.
16. Evans HH, Mencl J, Horng M-F, Ricanati M, Sanchez C, Hozier J (1986): Locus specificity in the mutability of L5178Y mouse lymphoma cells: The role of multilocus lesions. Proc Natl Acad Sci USA 83:4379-4383.
17. Gossen JA, de Leeuw WJF, Tan CH, Zwarthoff EC, Berends F, Lohman PHM, Knook DL, Vijg J (1989): Efficient rescue of integrated shuttle vectors from transgenic mice: A model for studying gene mutations in vivo. Proc Natl Acad Sci USA 86:7971-7975.
18. Gudas JM, Fridovich-Keil JL, Datta MW, Bryan J, Pardee AB (1992): Characterization of the murine thymidine kinase-encoding gene and analysis of transcription start point heterogeneity. Gene 118:205-216.
19. Habteyesus A, Nordenskjold A, Bohman C, Eriksson S (1991): Deoxynucleoside phosphorylating enzymes in monkey and human tissues show great similarities, while mouse deoxycytidine kinase has a different substrate specificity. Biochem Pharmacol 42:1829-1836.
20. Hogan B, Beddington R, Costantini F, Lacy E (1994): Isolation, culture, and manipulation of embryonic stem cells. In "Manipulating the Mouse Embryo, A Laboratory Manual," 2nd ed. Plainview, NY: Cold Spring Harbor Laboratory Press, pp. 253-290.
21. Hooper M, Hardy K, Handyside A, Hunter S, Monk M (1987): HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature 326:292-295.
22. Jones IM, Burkhart-Schultz K, Carrano AV (1985): A method to quantify spontaneous and in vivo induced thioguanine-resistant mouse lymphocytes. Mutat Res 147:97-105.
23. Joyner AL (1993): "Gene Targeting, A Practical Approach." New York: Oxford University Press.
24. Kohler SW, Provost GS, Fieck A, Kretz PL, Bullock WO, Sorge JA, Putman DL, Short JM (1991): Spectra of spontaneous and mutagen-induced mutations in the lacl gene in transgenic mice. Proc Natl Acad Sci USA 88:7958-7962.
25. Kronenberg A, Little JB (1989): Molecular characterization of thymidine kinase mutants of human cells induced by densely ionizing radiation. Mutat Res 211:215-224.
26. Liber HL, Yandell DW, Little JB (1989): A comparison of mutation induction at the tk and hprt loci in human lymphoblastoid cells; quantitative differences are due to an additional class of mutations at the autosomal tk locus. Mutat Res 216:9-17.
27. +/- mutants. Mutat Res 286:299-307.
28. Liechty MC, Hassanpour Z, Hozier JC, Clive D (1994): Use of microsatellite DNA polymorphisms on mouse chromosome 11 for in vitro analysis of thymidine kinase gene mutations. Mutagenesis 9:423-427.
29. Lin P-F, Lieberman HB, Yeh D-B, Xu T, Zhao S-Y, Ruddle FH (1985): Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol Cell Biol 5:3149-3156.
30. McCarrick JW, Parnes JR, Seong RH, Solter D, Knowles BB (1993): Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells. Transgenic Res 2:183-190.
31. Moore MM, Harrington-Brock K, Doerr CL, Dearfield KL (1989): Differential mutant quantitation at the mouse lymphoma tk and CHO hgprt loci. Mutagenesis 4:394-403.
32. Morham SG, Langenbach R, Loftin CD, Tiano HF, Vouloumanos N, Jennette JC, Mahler JF, Kluckman KD, Ledford A, Lee CA, Smithies O (1995): Prostaglandin synthase 2 gene disruption causes severe renal pathology in the mouse. Cell 83:473-482.
33. Sehlmeyer U, Wobus AM (1994): Lower mutation frequencies are induced by ENU in undifferentiated embryonic cells than in differentiated cells of the mouse in vitro. Mutat Res 324:69-76.
34. Skopek TR, Liber HL, Penman BW, Thilly WG (1978): Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: Possibility for a rapid human cell mutation assay. Biochem Biophys Res Commun 84:411-416.
35. Skopek TR, Kort KL, Marino DR (1995): Relative sensitivity of the endogenous hprt gene and lacl transgene in ENU-treated Big Blue™ B6C3F1 mice. Environ Mol Mutagen 26:9-15.
36. +/tk cell line is heterozygous for a codon 170 mutation in the p53 tumor supressor gene. Environ Mol Mutagen 27(Suppl 27):66.
37. Suzuki T, Hayashi M, Sofuni T, Myhr BC (1993): The concomitant detection of gene mutation and micronucleus induction by mitomycin C in vivo using lacZ transgenic mice. Mutat Res 285:219-224.
38. Tao KS, Urlando C, Heddle JA (1993): Comparison of somatic mutation in a transgenic versus host locus. Proc Natl Acad Sci USA 90:10681-10685.
39. Thomas KR, Capecchi MR (1987): Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51:503-512.
40. Udy GB, Evans MJ (1994): Microplate DNA preparation. PCR screening and cell freezing for gene targeting in embryonic stem cells. BioTechniques 17:887-894.
41. Van Sloun P, Bol S, Jansen J, Van Steeg H, Vreeling H, Lohman PHM (1996): Mutational studies in T-lymphocytes of rats and mice using endogenous genes as target loci. Environ Mol Mutagen 27(Suppl 27):79.
42. Willecke K, Teber T, Kucherlapati RS, Ruddle FH (1977): Human mitochondrial thymidine kinase is coded for by a gene on chromosome 16 of the nucleus. Somat Cell Genet 3:237-245.
43. Winegar RA, Lutze LH, Hamer JD, O'Loughlin KG, Mirsalis JC (1994): Radiation-induced point mutations, deletions and micronuclei in lacl transgenic mice. Mutat Res 307:479-487.
44. Yandell DW, Dryja TP, Little JB (1986): Somatic mutations at a heterozygous autosomal locus in human cells occur more frequently by allele loss than by intragenic structural alterations. Somat Cell Mol Genet 12:255-263.
45. Yandell DW, Dryja TP, Little JB (1990): Molecular genetic analysis of recessive mutations at a heterozygous autosomal locus in human cells. Mutat Res 229:89-102.