A monoclonal antibody against human lipoprotein lipase inhibiting heparin binding without affecting catalytic activity

Biochemistry and Cell Biology

Volumn 74, Issue 3, 1996, Pages 383-389

  Cadelis, François ^a   Julien, Pierre ^a   Valet, Jean Paul ^a   Deshaies, Yves ^b   Ven Murthy, M R ^b   Lupien, Paul J ^a  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

ELISA; Heparin; Immunoassay; Lipoprotein lipase; LPL cDNA; Monoclonal antibody

Indexed keywords

HEPARIN; LIPOPROTEIN LIPASE; MONOCLONAL ANTIBODY;

ADIPOSE TISSUE; ARTICLE; BINDING SITE; ENZYME LINKED IMMUNOSORBENT ASSAY; HEART MUSCLE; HUMAN; IMMUNOLOGY; METABOLISM; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ADIPOSE TISSUE; ANTIBODIES, MONOCLONAL; BINDING SITES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEPARIN; HUMANS; LIPOPROTEIN LIPASE; MOLECULAR WEIGHT; MYOCARDIUM;

EID: 0030330490     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o96-041     Document Type: Article

References (17)

1. Augustin, J., Freeze, H., Tejada, P., and Brown, W.V. 1978. A comparison of molecular properties of hepatic triglyceride lipase and lipoprotein lipase from human post-heparin plasma. J. Biol. Chem. 253: 2912-2920.
2. Bayer, E.A., and Wilchek, M. 1980. The use of avidin-biotin complex as a tool in molecular biology. Methods Biochem. Anal. 26: 1-45
3. Beisiegel, U., Weber, W., and Bengtsson-Olivecrona, G. 1991. Lipoprotein lipase enhances the binding of chylomicrons to low density lipoprotein receptor-related protein. Proc. Natl. Acad. Sci. U.S.A. 88: 8342-8346.
4. Belahsen, R., and Deshaies, Y. 1993. Involvement of insulinemia in the postprandial hypotriacylglycerolemia induced by prazosin in the rat. Metabolism, 42: 1301-1309.
5. Bengtsson-Olivecrona, G., and Olivecrona, T. 1991. Phospholipase activity of milk lipoprotein lipase. Methods Enzymol. 197: 345-356.
6. Bergeron, J., Julien, P., and Murthy, M.R.V. 1991. Expression de la lipoprotein lipase humaine: mutations et physiopathologie. Med. Sci. 7: 1061-1068.
7. Braun, J.E., and Severson, D.L. 1992. Regulation of the synthesis, processing and translocation of lipoprotein lipase. Biochem. J. 287: 337-347.
8. Cryer, A. 1987. Comparative biochemistry and physiology of lipoprotein lipase. In Lipoprotein lipase. Edited by J. Borensztajn. Evener Publishers Inc., Chicago, Ill. pp. 277-327.
9. dé St. Groth, S.F., and Scheidegger, D. 1980. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods, 35: 1-21.
10. Enerbäck, S., and Gimble, J.M. 1993. Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-transcriptional level. Biochim. Biophys. Acta, 1169: 107-125.
11. Etienne, J., Dosna, A.M., Noé, L., and Rossignol, M. 1979. Comparative inhibition properties of anti-lipoprotein lipase antiserum on some lipolytic activities. Artery, 6: 67-78.
12. Etienne, J., Noe, L., Millot, F., Lamelle, P., and Debray, J. 1987. Antibody against human lipoprotein lipase. Atherosclerosis, 64: 201-209.
13. Goers, J.W., Pedersen, M.E., Kern, P.A., Ong, J., and Schotz, M.C. 1987. An enzyme-linked immunoassay for lipoprotein lipase. Anal. Biochem. 166: 27-35.
14. Goldberg, I.J., Paterniti, J.R., Jr., France, D.S., Martinelli, G., and Cornicelli, J.A. 1986. Production and use of an inhibitory monoclonal antibody to human lipoprotein lipase. Biochim. Biophys. Acta, 878: 168-176.
15. Hager, D.A., and Burgess, R.R. 1980. Elution of proteins from sodium dodecyl sulfate - polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal. Biochem. 109: 76-86.
16. Hata, A., Ridinger, D.N., Sutherland, S., Emi, M., Shuhua, Z., Myers, R.L., Ren, K., Cheng, T., Inoue, I., Wilson, D.E., Iverius, P.H., and Lalouel, J.M. 1993. Binding of lipoprotein lipase to heparin. Identification of five critical residues in two distinct segments of the amino-terminal domain. J. Biol. Chem. 268: 8447-8457.
17. Hayashi, R., Tajima, S., and Yamamoto, A. 1986. Purification and characterization of lipoprotein lipase from human postheparin plasma and its comparison with purified bovine milk lipoprotein lipase. J. Biochem. (Tokyo), 100: 319-331.