A simple and rapid technique for the immunofluorescence confocal microscopy of intact Arabidopsis root tips

Cytobios

Volumn 1996, Issue 349, 1996, Pages 71-78

  Harper, J D I ^a   Holdaway, N J ^a   Brecknock, S L ^a   Busby, C H ^b   Overall, R L ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

Antibody; Brefeldin A; Callose; Calmodulin; Image analysis; Immunocytochemistry; Microtubules; MPM 2; Phragmoplasts

Indexed keywords

BREFELDIN A; CYCLOPENTANE DERIVATIVE;

ARABIDOPSIS; ARTICLE; CONFOCAL MICROSCOPY; DRUG EFFECT; FLUORESCENT ANTIBODY TECHNIQUE; MICROTUBULE; MITOSIS; MITOSIS SPINDLE; PLANT ROOT; ULTRASTRUCTURE;

ARABIDOPSIS; BREFELDIN A; CYCLOPENTANES; FLUORESCENT ANTIBODY TECHNIQUE; MICROSCOPY, CONFOCAL; MICROTUBULES; MITOSIS; MITOTIC SPINDLE APPARATUS; PLANT ROOTS;

EID: 0030325405     PISSN: 00114529     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. BASKIN T. I., Busby C. H., Fowke L. C., Sammut M. and Gubler F. 1992. Improvements in immunostaining samples embedded in methacrylate: localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. Planta 187 405-13.
2. BENFEY P. N. and Shiefelbein J. W. 1994 Getting to the root of plant development: genetics of Arabidopsis root formation. Trends Genet. 19 84-8.
3. BINAROVA P., Rennie P. and Fowke L. 1994. Probing plant microtubule organising centres with MPM-2 in dividing cells of higher plants using immunofluorescence and immunogold techniques. Protoplasma 180 106-17.
4. BROWN R. C. and Lemmon B. E. 1995. Methods in plant immunolight microscopy. In Methods in Cell Biology. Edited by D. W. Galbraith, H. J. Bohnert and D. P. Bourque. Academic Press 49 85-107.
5. BURON I. M. and Garcia-Herdugo G. 1983. Experimental analysis of cytokinesis: comparison of inhibition induced by 2,6-dichlorobenzonitrile and caffeine. Protoplasma 118 192-8.
6. DAVIS F. M., Tsao T. Y., Fowler S. K. and Rao P. N. 1983. Monoclonal anti-bodies to mitotic cells. Proc. natn Acad. Sci USA 80 2926-30.
7. DERKSEN J., Jeucken G., Trass J. A. and Van Lammeren A. A. M. 1986. The microtubular skeleton in differentiating root tips of Raphanus sativus L. Acta Bot. Neerlandica 36 223-31.
8. DRIOUICH A., Zhang G. F. and Staehelin L. A. 1993. Effect of brefeldin A on the structure of the Golgi apparatus and on the synthesis and secretion of proteins and polysaccharides in sycamore maple (Acer pseudoplantus) suspension-cultured cells. Plant Physiol. 101 1363-73.
9. ERICSON M. E. and Carter J. V. 1996. Immunolabelled microtubules and microfilaments are visible in multiple cell layers of rye root tip sections. Protoplasma 191 215-9.
10. GEITMANN A., Wojciechowicz K. and Cresti M. 1996. Inhibition of intracellular pectin transport in pollen tubes by monensin, brefeldin A and cytochalasin D. Bot. Acta 109 73-81.
11. GUBLER F. 1989. Immunofluorescence localisation of microtubules in plant root tips embedded in butyl-methyl methacrylate. Cell Biol. Int. Rep. 13 137-45.
12. HOGETSU T. and Oshima Y. 1986. Immunofluorescence of microtubule arrangement in root cells of Pisum sativum L. var Alaska. Plant Cell Physiol. 27 939-45.
13. HOLDAWAY N. J., White R. G. and Overall R. L. 1995. Is the recovery of microtubule orientation in pea roots dependent on the cell wall? Cell Biol. Int. 19 913-9.
14. HUSH J. M. Hawes C. R. and Overall R. L. 1990. Interphase microtubule re-orientation predicts a new cell polarity in wounded pea roots. J. Cell Sci. 96 47-61.
15. JABLONSKY P. P., Grolig F., Perkin J. L. and Williamson R. E. W. 1991. Properties of monoclonal antibodies to plant calmodulin. Plant Sci. 76 175-84.
16. LLOYD C. W. 1987. The plant cytoskeleton: impact of fluorescence microscopy. A. Rev. Plant Physiol. 38 98-132.
17. MEYER Y. and Herth W. 1978. Chemical inhibition of cell wall formation and cytokinesis but not nuclear division, in protoplasts of Nicotiana tabacum L. cultured in vitro. Planta 142 253-62.
18. STAIGER C. J. and Doonan J. H. 1993. Cell division in plants. Curr. Opin. Cell Biol. 5 226-31.
19. TIWARI C. S., Wick S. M., Williamson R. E. and Gunning B. E. S. 1984. The cytoskeleton and integration of cellular function in cells of higher plants. J cell Biol. 99 63s-69s.
20. TRAAS J. A., Beven A. F., Doonan J. H., Cordewener J. and Shaw J. 1992. Cell-cycle-dependent changes in labelling of specific phosphoproteins by the monoclonal antibody MPM-2 in plant cells. Plant J. 2 723-32.
21. WESTENDORF K. M., Rao P. N. and Gerace L. 1994. Cloning of cDNAs for M-phase phosphoproteins recognised by the MPM2 monoclonal antibody and determination of the phosphorylated epitope. Proc. natn Acad. Sci. U.S.A. 91 714-8.
22. WICK S. M. 1985. The higher plant mitotic apparatus: redistribution of microtubules, calmodulin and microtubule initiating material during its establishment. Cytobios 285-94.
23. YASUHARA H., Sonobe S. and Shibaoka H. 1995. Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells. Eur. J. Cell Biol. 66 274-81.