Secretion of active Fc fragments of immunoglobulin E directed by the yeast invertase signal sequence in mammalian cells

Tohoku Journal of Experimental Medicine

Volumn 180, Issue 4, 1996, Pages 297-308

  Kamiya, Toshio ^a   Sugio, Shigetoshi ^a   Yamanouchi, Kouichi ^a   Kagitani, Yoshio ^a  

^a Green Cross Company   (South Korea)

Author keywords

Endoplasmic reticulum; Heterologous expression; IgE Fc; Signal sequence; Yeast invertase

Indexed keywords

BETA FRUCTOFURANOSIDASE; GLYCOSIDASE; IMMUNOGLOBULIN E; IMMUNOGLOBULIN FC FRAGMENT; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL LINE; CHEMISTRY; CHO CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYMOLOGY; GENE EXPRESSION; GENE VECTOR; GENETICS; HAMSTER; HAPLORHINI; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SECRETION; SERODIAGNOSIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BETA-FRUCTOFURANOSIDASE; CELL LINE; CHO CELLS; CRICETINAE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; GLYCOSIDE HYDROLASES; HAPLORHINI; HUMANS; IMMUNOGLOBULIN E; IMMUNOGLOBULIN FC FRAGMENTS; MOLECULAR SEQUENCE DATA; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TUMOR CELLS, CULTURED;

EID: 0030320961     PISSN: 00408727     EISSN: None     Source Type: Journal    
DOI: 10.1620/tjem.180.297     Document Type: Article

References (20)

1. Basu, M., Hakimi, J., Dharm, E., Kondas, J.A., Tsien, W.-H., Pilson, R.S., Lin, P., Gilfillan, A., Haring, P., Braswell, E.H., Nettleton, M.Y. & Kochan, J.P. (1993) Purification and characterization of human recombinant IgE-Fc fragments that bind to the human high affinity IgE receptor. J. Biol. Chem., 268, 13118-13127.
2. Bergh, M.L.E., Cepko, C.L., Wolf, D. &. Robbins, P.W. (1987) Expression of the Saccharomyces cerevisiae glycoprotein invertase in mouse fibroblasts: Glycosylation, secretion, and enzymatic activity. Proc. Natl. Acad. Sci. USA, 84, 3570-3574.
3. Bird, P., Gething, M.-J. & Sambrook, J. (1987) Translocation in yeast and mammalian cells: Not all signal sequences are functionally equivalent. J. Cell Biol., 105, 2905-2914.
4. Dobberstein, B. (1994) Protein transport: On the beaten pathway. Nature, 367, 599-600.
5. Gilmore, R. (1993) Protein translocation across the endoplasmic reticulum: A tunnel with toll booths at entry and exit. Cell, 75, 589-592.
6. Harlow, E. & Lane, D. (1988) Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Jungnickel, B. & Rapoport, T.A. (1995) A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane. Cell, 82, 261-270.
8. Karaplis, A.C., Lim, S.-K., Baba, H., Arnold, A. & Kronenberg, H.M. (1995) Inefficient membrane targeting, translocation, and proteolytic processing by signal peptidase of a mutant preproparathyroid hormone protein. J. Biol. Chem., 270, 1629-1635.
9. Linsley, P.S., Brady, W., Grosmaire, L., Aruffo, A., Damle, N.K. & Ledbetter, J.A. (1991) Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. J. Exp. Med., 173, 721-730.
10. Romanos, M.A., Scorer, C.A. & Clare, J.J. (1992) Foreign gene expression in yeast: A review. Yeast, 8, 423-488.
11. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. Sanger, F., Nicklen, S. & Coulson, A.R. (1977) DNA sequencing with chainterminating inhibitors. Proc. Natl. Acad. Sci. USA, 74, 5463-5467.
13. Seed, B. (1987) An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2. Nature, 329, 840-842.
14. Su, K., Stoller, T., Rocco, J., Zemsky, J. & Green, R. (1993) Pre-Golgi degradation of yeast prepro-α-factor expressed in a mammalian cell. J. Biol. Chem., 268, 14301-14309.
15. Sutton, B.J. & Gould, H.J. (1993) The human IgE network. Nature, 366, 421-428.
16. Tanaka, M., Suda, T., Takahashi, T. & Nagata, S. (1995) Expression of the functional soluble form of human Fas ligand in activated lymphocytes. EMBO J., 14, 1129-1135.
17. Tuite, M.F. & Freedman, R.B. (1994) Improving secretion of recombinant proteins from yeast and mammalian cells: Rational or empirical design ? Trends Biotechnol., 12, 432-434.
18. Walter, P. & Johnson, A.E. (1994) Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu. Rev. Cell Biol., 10, 87-119.
19. Wedlock, N., Furmaniak, J., Fowler, S., Kiso, Y., Bednarek, J., Baumann-Antczak, A., Morteo, C., Sudbery, P., Hinchcliff, A. & Smith, B.R. (1993) Expression of human thyroid peroxidase in the yeasts Saccharomyces cerevisiae and Hansenula polymorpha. J. Mol. Endocrinol., 10, 325-336.
20. Young, R.J., Owens, R.J., Mackay, G.A., Chan, C.M.W., Shi, J., Hide, M., Francis, D. M., Henry, A.J., Sutton, B.J. & Gould, H.J, (1995) Secretion of recombinant human IgE-Fc by mammalian cells and biological activity of glycosylation site mutants. Protein Eng., 8, 193-199.