A novel method for viewing heavy metal stained and embedded biological tissue by field emission scanning electron microscopy

Scanning Microscopy

Volumn 10, Issue 1, 1996, Pages 111-119

  Richards, R Geoffrey ^a   Ap Gwynn, Iolo ^b  

^a AO RESEARCH INSTITUTE   (Switzerland)

^b ABERYSTWYTH UNIVERSITY   (United Kingdom)

Author keywords

Atomic number contrast; Backscattered electron imaging; Field emission; Pathology; Scanning electron microscopy

Indexed keywords

ARTICLE; ELECTRON TRANSPORT; IMAGE PROCESSING; MATHEMATICAL ANALYSIS; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALS; METALS; MICE; MICROSCOPY, ELECTRON, SCANNING; RATS; STAINING AND LABELING; TISSUE EMBEDDING;

EID: 0030316469     PISSN: 08917035     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Drescher H, Reimer L, Seidel H (1970) Backscatter coefficient and secondary electron yield of 10-100 keV electrons and relation to scanning electron microscopy. Z angew Phys 29: 331-336.
2. Echlin P, Broers AN, Gee W (1980) Improved resolution of sputter coated films. Scanning Electron Microsc 1980; I: 163-170.
3. Everhart TE, Wells OC, Oatley CW (1959) Factors affecting contrast and resolution in the scanning electron microscope. J Electron Control 7: 97-111.
4. Goldstein JI, Newbury DE, Echlin P, Joy DC, Romig AD, Lyman CE, Fiori C, Lifshin E (1992) Scanning Electron Microscopy and X-ray Microanalysis. 2nd Edition. Plenum Press, New York. pp. 69-146.
5. Kanaya K, Okayama S (1972) Penetration and energy-loss theory of electrons in solid targets. J Phys D: Appl Phys 5: 43-58.
6. Kushida H, Kushida T, Nagato Y, Ogura K (1982) An improved method for both light and scanning electron microscopy in backscattered electron mode of identical sites in semi-thin sections embedded in GMA and Quetol 523. J Electron Microsc 31: 202-205.
7. Nanci A, Zalzal S, Smith CE (1990) Routine use of backscattered electron imaging to visualize cytochemical and autoradiographic reactions in semi-thin plastic sections. J Histochem Cytochem 38: 403-414.
8. Reynolds EA (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Cell Biol 17: 208-212.
9. Richards RG, ap Gwynn I (1995) Backscattered electron imaging of the undersurface of resin-embedded cells by field emission scanning electron microscopy. J Microsc 177: 43-52.
10. Scala C, Pasquinelli G, Martegani F, Laschi R (1985) Use of secondary electron detectors for compositional studies on embedded biological material Scanning Electron Microsc 1985; IV: 1709-1718.
11. Scala C, Cenacchi G, Apkarian RP, Preda P, Pasquinelli G (1990) Correlative light microscopy, scanning electron microscopy, and transmission electron microscopy of osmium macerated biological tissues. J Electron Microsc 39: 508-510.
12. Scala C, Cenacchi G, Preda P, Vici M, Apkarian RP, Pasquinelli G (1991) Conventional and high resolution scanning electron microscopy of biological sectioned material. Scanning Microsc 5: 135-145.
13. Watson ML (1958) Staining of tissue section for electron microscopy with heavy metals. J Biophys Biochem Cytol 4: 475-478.
14. Wing-Ling NG, Ma L, So KF (1982) Preparation of Epon-embedded renal tissue for scanning electron microscopy. J Clin Pathol 35: 1384-1387.