A Yohimbine-dependent, UK14,304 induced ion transient in HT29 cells studied by X-ray micro analysis

Scanning Microscopy

Volumn 10, Issue 1, 1996, Pages 293-299

  Zhang, Wei ^a   Roomans, Godfried M ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

2 adrenoceptors; HT29 cells; RX821002; UK14,304; X ray microanalysis; Yohimbine

Indexed keywords

ALPHA 2 ADRENERGIC RECEPTOR; YOHIMBINE;

ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL STRAIN HT29; CELL ULTRASTRUCTURE; CHLORIDE TRANSPORT; HUMAN; HUMAN CELL; POTASSIUM CELL LEVEL; PRIORITY JOURNAL; RECEPTOR DENSITY; SODIUM CELL LEVEL; SODIUM TRANSPORT; X RAY MICROANALYSIS;

CALCIUM; CHLORIDES; ELECTRON PROBE MICROANALYSIS; HT29 CELLS; HUMANS; INTESTINAL MUCOSA; ION TRANSPORT; QUINOXALINES; RECEPTORS, ADRENERGIC, ALPHA-2; SODIUM; YOHIMBINE;

EID: 0030316456     PISSN: 08917035     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Beesley JE, Hall SR, Page MJ, Affleck K (1995) Early elemental and ionic changes in cultured cells after stimulation with epidermal growth factor. Scanning Microsc 9: 231-238.
2. 2-adrenoceptor antagonist therapy. TiPS 13: 277-282.
3. Briggs GM, Meier CJ (1986) Effect of yohimbine on action potentials recorded from isolated canine ventricular myocytes. Eur J Pharmacol 127: 125-128.
4. Bylund DB (1992) Subtypes of alpha 1- and alpha 2-adrenergic receptors. FASEB J 6: 832-839.
5. Bylund DB, Ray-Prenger C, Murphy TJ (1989) Alpha-2A and alpha-2B adrenergic receptor subtypes: Antagonist binding in tissues and cell lines containing only one subtype. J Pharmacol Exp Ther 245: 600-607.
6. Bylund DB, Blaxall HS, Iversen LJ, Caron MG, Lefkowitz RJ, Lomasney JW (1992) Pharmacological characteristics of alpha 2-adrenergic receptors: Comparison of pharmacologically defined subtypes with subtypes identified by molecular cloning. Mol Pharmacol 42: 1-5.
7. + exchange via a cAMP-dependent mechanism. Am J Physiol 28: F977-F985.
8. Cox HM, Cuthbert AW (1989) Antisecretory activity of the alpha 2-adrenoceptor agonist, xylazine in rat jejunal epithelium. Naunyn Schmiedebergs Arch Phar- macol 339: 669-674.
9. 2A-adrenergic receptor in the HT29 cell line: Effects of insulin and growth factors. J Biol Chem 266: 14359-14366.
10. Fogg KE, Higgs NB, Warhurst G (1994) Involvement of calmodulin in Ca-activated K efflux in the human colonic cell line, HT29-19A. Biochim Biophys Acta 1221: 185-192.
11. Hall SR, Foxwell N, Beesley JE (1994) Preparation of cultured cells for microscopical investigation of responses to pharmacological reagents. Cells Mater 4: 81-89.
12. Hildebrand KR, Lin G, Murtaugh MP, Brown DR (1993) Molecular characterization of alpha 2-adrenergic receptors regulating intestinal electrolyte transport. Mol Pharmacol 43: 23-29.
13. Kunzelmann K, Grolik M, Greger R (1992) cAMP-dependent activation of small-conductance Cl channels in HT29 colon carcinoma cells. Pflügers Arch 421: 230-237.
14. Lipicky RJ, Gilbert DL, Ehrenstein G (1978) Effects of yohimbine on squid axons. Biophys J 24: 405-422.
15. Lohrmann E, Cabantchik ZI, Greger R (1992) Transmitter-induced changes of the membrane voltage of HT29 cells. Pflügers Arch 421: 224-229.
16. Matthews JB, Smith JA, Tally KJ, Awtrey CS, Nguyen H, Rich J, Madara JL (1994) Na-K-2Cl cotransport in intestinal epithelial cells: Influence of chloride efflux F-actin on regulation of cotransporter activity and bumetanide binding. J Biol Chem 269: 15703-15709.
17. 2-adrenoceptor turnover and metabolism using the adenocarcinoma cell line HT29. Mol Pharmacol 32: 646-654.
18. Remaury A, Larrouy D, Daviaud D, Rouot B, Paris H (1993) Coupling of the alpha 2-adrenergic receptor to the inhibitory G-protein Gi and adenylate cyclase in HT29 cells. Biochem J 292: 283-288.
19. Roomans GM (1988) Quantitative X-ray microanalysis of biological specimens. J Electron Microsc Techn 9: 19-44.
20. Roomans GM, Sevéus L (1977) Preparation of thin cryosectioned standards for quantitative microprobe analysis. J Submicrosc Cytol 9: 31-35.
21. Sellin JH, De SR (1987) Regulation of Na-Cl absorption in rabbit proximal colon in vitro. Am J Physiol 252: G52-G55.
22. 2-adrenergic receptors in the human cell line, HT29: Characterization with the full agonist radioligand [3H]UK-14,304 and inhibition of adenylate cyclase. Mol Pharmacol 28: 422-430.
23. Von Euler A, Roomans GM (1992) Ion transport in colon cancer cultures studied by X-ray microanalysis. Cell Biol Int Rep 16: 293-306.
24. Von Euler A, Wróblewski R, Roomans GM (1992) Correction for extraneous background in X-ray microanalysis of cell cultures. Scanning Microsc 6: 451-456.
25. Von Euler A, Pålsgård E, Vult von Steyern C, Roomans GM (1993) X-ray microanalysis of epithelial and secretory cells in culture. Scanning Microsc 7: 191-202.
26. Yarrow S, Ferrar JA, Cox HM (1991) The effects of capsaicin upon electrogenic ion transport in rat descending colon. Naunyn Schmiedebergs Arch Pharmacol 344: 557-563.