Measurement of bacterial production in stream-bed sediments via leucine incorporation

FEMS Microbiology Ecology

Volumn 21, Issue 4, 1996, Pages 313-325

  Marxsen, Jürgen ^a  

^a MAX PLANCK INSTITUTE FOR EVOLUTIONARY BIOLOGY   (Germany)

Author keywords

bacterial production; leucine incorporation; sediment; sediment core perfusion technique; stream

Indexed keywords

LEUCINE;

ARTICLE; BACTERIAL GROWTH; BENTHOS; BIOMASS; CONTROLLED STUDY; ECOLOGY; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; SEDIMENT;

BACTERIAL PRODUCTION; LEUCINE INCORPORATION; SEDIMENT; STREAM BED;

GERMANY, HESSE, BREITENBACH;

EID: 0030301422     PISSN: 01686496     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-6496(96)00067-0     Document Type: Article

References (36)

1. [1] Pomeroy, L.R. (1974) The ocean's food web, a changing paradigm. BioScience 24, 499-504.
2. [2] Sander, B.C. and Kalff, J. (1993) Factors controlling bacterial production in marine and freshwater sediments. Microb. Ecol. 26, 79-99.
3. [3] Kirchman, D., Knees, E. and Hodson, R. (1985) Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Appl. Environ. Microbiol. 49, 599-607.
4. [4] Simon, M. and Azam, F. (1989) Protein content and protein synthesis rates of planktonic marine bacteria. Mar. Ecol. Prog. Ser. 51, 201-213.
5. [5] Riemann, B. and Bell, R.T. (1990) Advances in estimating bacterial biomass and growth in aquatic systems. Arch. Hydrobiol. 118, 385-402.
6. 3H]valine into protein of freshwater bacteria: uptake kinetics and intracellular isotope dilution. Appl. Environ. Microbiol. 58, 3638-3646.
7. 14C]leucine incorporation rate. Microb. Ecol. 26, 201-217.
8. [8] Moriarty, D.J.W., Skyring, G.W., O'Brien, G.W. and Heggie, D.T. (1991) Heterotrophic bacterial activity and growth rates in sediments of the continental margin of eastern Australia. Deep-Sea Res. 38, 693-712.
9. [9] Moran, M.A. and Hodson, R.E. (1992) Contributions of three subsystems of a freshwater marsh to total bacterial secondary productivity. Microb. Ecol. 24, 161-170.
10. [10] Tuominen, L. and Kairesalo, T. (1992) A method for measuring the uptake of thymidine and leucine in sediment. Aqua Fenn. 22, 43-48.
11. [11] Duyl, F.C. van and Kop, A.J. (1994) Bacterial production in North Sea sediments: clues to seasonal and spatial variations. Mar. Biol. 120, 323-337.
12. [12] Kairesalo, T., Tuominen, L., Hartikainen, H. and Rankinen, K. (1995) The role of bacteria in the nutrient exchange between sediment and water in a flow-through system. Microb. Ecol. 29, 129-144.
13. [13] Bååth, E. (1994) Measurement of protein synthesis by soil bacterial assemblages with the leucine incorporation technique. Biol. Fert. Soils 17, 147-153.
14. 2-Dunkelfixierung und Eintrag von partikulärem organischen Material. Arch. Hydrobiol. Suppl. 57, 461-533.
15. [15] Hall, K.J., Kleiber, P.M. and Yesaki, I. (1972) Heterotrophic uptake of organic solutes by microorganisms in the sediments. Mem. Ist. Ital. Idrobiol. 29 (Suppl.), 441-471.
16. [16] Meyer-Reil, L.-A. (1986) Measurement of hydrolytic activity and incorporation of dissolved organic substrates by microorganisms in marine sediments. Mar. Ecol. Prog. Ser. 31, 143-149.
17. [17] Novitzky, J.A. (1983) Microbial activity at the sediment-water interface in Halifax Harbour, Can. Appl. Environ. Microbiol. 45, 1761-1766.
18. [18] Fiebig, D.M. (1992) Fates of dissolved free amino acids in groundwater discharged through stream-bed sediments. Hydrobiologia 235-236, 311-319.
19. [19] Marxsen, J. and Fiebig, D.M. (1993) Use of perfused cores for evaluating extracellular enzyme activity in stream-bed sediments. FEMS Microbiol. Ecol. 13, 1-12.
20. 3H]thymidine into DNA. J. Microbiol. Methods 2, 57-72.
21. 3H]cytidine into cultured lymphocytes. Biochem. J. 125, 721-732.
22. [22] Pollard, P.C. and Moriarty, D.J.W. (1984) Validity of the tritiated thymidine method for estimating bacterial growth rates: Measurement of isotope dilution during DNA synthesis. Appl. Environ. Microbiol. 48, 1076-1083.
23. 14C]glucose incorporation by aquatic bacteria. Acta Microbiol. Pol. 38, 75-89.
24. [24] Sachs, L. (1972) Statistische Methoden, 2nd edn., 105 pp. Springer, Berlin.
25. [25] Sachs, L. (1978) Angewandte Statistik, 5th edn., 552 pp. Springer, Berlin.
26. [26] Rausch, T. (1981) The estimation of micro-algal protein content and its meaning to the evaluation of algal biomass. I. Comparison of methods for extracting protein. Hydrobiologia 78, 237-251.
27. [27] Kairesalo, T., Lehtovaara, A. and Saukkonen, P. (1992) Littoral-pelagic interchange and the decomposition of dissolved organic matter in a polyhumic lake. Hydrobiologia 229, 199-224.
28. 3H]Leucine incorporation methodology to estimate epiphytic bacterial biomass production. Microb. Ecol. 29, 63-70.
29. [29] Looij, A. van and Riemann, B. (1993) Measurement of bacterial production in coastal marine environments using leucine: application of a kinetic approach to correct for isotope dilution. Mar. Ecol. Prog. Ser. 102, 97-104.
30. [30] Moriarty, D.J.W. (1986) Measurement of bacterial growth rates in aquatic systems from rates of nucleic acid synthesis. Adv. Microb. Ecol. 9, 245-292.
31. [31] Marxsen, J. and Schmidt, H.-H. (1993) Extracellular phosphatase activity in sediments of the Breitenbach, a Central European mountain stream. Hydrobiologia 253, 207-216.
32. [32] Fiebig, D.M. (1995) Groundwater discharge and its contribution of dissolved organic carbon to an upland stream. Arch. Hydrobiol. 134, 129-156.
33. [33] Findlay, S.E.G., Meyer, J.L. and Risley, R. (1986) Benthic bacterial biomass and production in two blackwater rivers. Can. J. Fish. Aquat. Sci. 43, 1271-1276.
34. [34] Austin, H.K. and Findlay, S. (1989) Benthic bacterial biomass and production in the Hudson River Estuary. Microb. Ecol. 18, 105-116.
35. [35] Kaplan, L.A. and Bott, T.L. (1989) Diel fluctuations in bacterial activity on streambed substrata during vernal alga1 blooms: effects of temperature, water chemistry, and habitat. Limnol. Oceanogr. 34, 718-733.
36. [36] Marxsen, J. (1988) Evaluation of the importance of bacteria in the carbon flow of a small open grassland stream, the Breitenbach. Arch. Hydrobiol. 111, 339-350.