A protocol for the efficient screening of putatively transformed plants for bar, the selectable marker gene, using the polymerase chain reaction

Plant Molecular Biology Reporter

Volumn 14, Issue 4, 1996, Pages 363-368

  Vickers, Joan E ^a,c   Graham, Glenn C ^a   Henry, Robert J ^b  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b SOUTHERN CROSS UNIVERSITY   (Australia)

^c CSIRO   (Australia)

Author keywords

Bar gene; Barley; PCR screening; Transformation

Indexed keywords

EMBRYOPHYTA; HORDEUM VULGARE SUBSP. VULGARE;

EID: 0030298606     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02673368     Document Type: Article

References (9)

1. Barnes, W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. (USA) 91:2216-2220.
2. Bennett, M.D., and J.B. Smith. 1976. Nuclear DNA amounts in angiosperms. Phil. Trans. Roy. Soc. Lond. 274:227-274.
3. Boehringer Mannheim. 1995. PCR Applications Manual . Boehringer Mannheim GmbH, Biochimica. Germany. 194 pp.
4. Graham, G.C., P. Mayers, and R.J. Henry. 1994. A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques 16:48-50.
5. Thompson, C.J., N.R. Movva, R. Tizard, R. Crameri, J.E. Davies, M. Lauwereys, and J. Botterman. 1987. Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus . EMBO J. 6:2519-2523.
6. Vickers, J.E., S. Weining, S.E. Hamilton, A. Shaw, A. Walmsley, J. de Jersey, P.A. Inkerman & R.J. Henry. 1994. Thermostable a-amylase cloned for genetic transformation of barley, pp. 170-173 in: Proceedings of the 44th Australian Cereal Chemistry Conference, Ballarat, Australia . (J.F. Panozzo, P. G. Downie, eds.).
7. Walmsley, A.M., R.J. Henry, and R.G. Birch. 1995. Optimization of tissue culture conditions for transformation studies using immature embryos of Australian barley cultivars. Aust. J. Bot. 43:499-504.
8. Weining, S., and P. Langridge. 1991. Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theor. Appl. Genet. 82:209-216.
9. White, J., S.-Y.P. Chang, M.J. Bibb, and M.J. Bibb. 1990. A cassette containing the bar gene of Streptomyces hygroscopicus: A selectable marker for plant transformation. Nucl. Acids Res. 18:1062.