High-level expression, purification, and crystallization of recombinant spinach glycolate oxidase in Escherichia coli

Protein Expression and Purification

Volumn 8, Issue 3, 1996, Pages 295-298

  Stenberg, Kaj ^a   Lindqvist, Ylva ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030294805     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0103     Document Type: Article

References (12)

1. 1. Volokita, M., and Somerville, C. R. (1987) The primary structure of spinach glycolate oxidase deduced from the DNA sequence of a cDNA clone. J. Biol. Chem. 262, 15825-15828.
2. 2. Macheroux, P., Massey, V., and Thiele, D. J. (1991) Expression of spinach glycolate oxidase in Saccharomyces cerevisiae: Purification and characterization. Biochemistry 30, 4612-4619.
3. 3. Macheroux, P., Mulrooney, S. B., Williams, C. H., Jr., and Massey, V. (1992) Direct expression of active spinach glycolate oxidase in Escherichia coli. Biochim. Biophys. Acta 1132, 11-16.
4. 4. Macheroux, P., Kieweg, V., Massey, V., Söderlind, E., Stenberg, K., and Lindqvist, Y. (1993) Role of tyrosine 129 in the active site of spinach glycolate oxidase. Eur. J. Biochem. 213, 1047-1054.
5. 5. Stenberg, K., Clausen, T., Lindqvist, Y., and Macheroux, P. (1995) Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase. Eur. J. Biochem. 228, 408-416.
6. 6. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
7. 7. Kerr, M. W., and Groves, D. (1975) Purification and properties of glycollate oxidase from Pisum Sativum leaves. Phytochemistry 14, 359-362.
8. 8. Lindqvist, Y., and Bränden, C-I. (1979) Preliminary crystallographic data for glycolate oxidase from spinach. J. Biol. Chem. 254, 7403-7404.
9. 9. Otwinowski, Z. (1993) DENZO. In "Data Collection and Processing" (Sawyer, L., Isaac, N., Bailey, S., Eds.), pp. 80-86, SERC Daresbury Laboratory, Warrington, UK.
10. 10. Zelitch, I., and Ochoa, S. (1953) Oxidation and reduction of glycolic and glyoxylic acids in plants. J. Biol. Chem. 201, 707-718.
11. 11. Frigerio, N. A., and Harbury, H. A. (1958) Preparation and some properties of crystalline glycolic acid oxidase of spinach. J. Biol. Chem. 231, 135-157.
12. 12. Mertens, N., Remault, E., and Fiers, W. (1995) Tight transcriptional control mechanism ensures stable high-level expression from T7 promotor-based expression plasmids. Bio / technology 13, 175-179.