Comparison of two convection-aided protein adsorption methods using porous membranes and perfusion beads

Biotechnology Progress

Volumn 12, Issue 6, 1996, Pages 869-872

  Kubota, Noboru ^a   Konno, Yoshitaka ^a   Miura, Suguru ^a   Saito, Kyoichi ^a   Sugita, Kazuyuki ^a   Watanabe, Kohei ^b   Sugo, Takanobu ^c  

^a CHIBA UNIVERSITY   (Japan)

^b ASAHI CHEMICAL INDUSTRY CO   (Japan)

^c TAKASAKI ADVANCED RADIATION RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORPTION; CHROMATOGRAPHY; ION EXCHANGE; ION EXCHANGE MEMBRANES; MOLECULAR STRUCTURE; POROUS MATERIALS; PRESSURE EFFECTS;

ANION EXCHANGE INTERACTION; BOVINE SERUM ALBUMIN SOLUTION; MEMBRANE CHROMATOGRAPHY; PERFUSION BEADS; PERFUSION CHROMATOGRAPHY; PROTEIN RECOVERY;

PROTEINS;

BOVINAE;

EID: 0030293514     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp960076s     Document Type: Article

References (5)

1. Afeyan, N. B.; Gordon, N. F.; Mazsaroff, I.; Varady, L.; Fulton, S. P. Flow-Through Particles for the High-Performance Liquid Chromatographic Separation of Biomolecules: Perfusion Chromatography. J. Chromatogr. 1990, 519, 1-29.
2. Brandt, S.; Goffe, R. A.; Kessler, S. B.; O'Connor, J. L.; Zale, S. E. Membrane-Based Affinity Technology for Commercial Scale Purifications. Bio / Technology 1988, 6, 779-782.
3. PerSeptive Biosystems Co. POROS Columns and Media Catalog; PerSeptive Biosystems Co.: Cambridge, MA, 1994.
4. Pharmacia Biotech Co. Sepharose Catalog; Pharmacia Biotech Co.: Uppsala, Sweden, 1994.
5. Tsuneda, S.; Saito, K.; Furusaki, S.; Sugo, T. High-Throughput Processing of Proteins Using a Porous and Tentacle AnionExchange Membrane. J. Chromatogr. A 1995, 689, 211-218.