The use of a PCR cloning and screening strategy to identify lambda clones containing the hupB gene of Anabaena sp. strain PCC7120

Journal of Microbiological Methods

Volumn 27, Issue 2-3, 1996, Pages 175-182

  Gubili, Jyothirmai ^a   Borthakur, Dulal ^a  

^a UNIVERSITY OF HAWAII   (United States)

Author keywords

Anabaena; hupB gene; Hydrogenase; PCR cloning

Indexed keywords

HYDROGENASE;

AMINO ACID SEQUENCE; ANABAENA; ARTICLE; BACTERIOPHAGE LAMBDA; CONTROLLED STUDY; DNA SEQUENCE; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING;

EID: 0030293190     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(96)00947-5     Document Type: Article

References (17)

1. [1] Haselkorn, R. (1978) Heterocysts. Ann. Rev. Plant Physiol. 29, 319-344.
2. [2] Papen, H., Kentemich, T., Schmülling, T. and Bothe, H. (1986) Hydrogenase activities in cyanobacteria. Biochimie 68, 121-132.
3. [3] Przybyla, A.E., Robbins, J., Menon, N. and Peck, H.R. Jr. (1992) Structure function relationships among nickel-containing hydrogenases. FEMS Microbiol. Rev. 88, 109-136.
4. [4] Wu, L.-F. and Mandrand, M.A. (1993) Microbial hydrogenases: primary structure, classification, signatures and phylogeny. FEMS Microbiol. Rev. 104, 243-270.
5. 2 uptake hydrogenases. Arch. Microbiol. 161, 1-10.
6. [6] Voordouw, G. (1992) Evolution of hydrogenase genes. Adv. Inorg. Chem. 38, 397-422.
7. [7] Maier, T., Jacobi, A., Sauter, M. and A. Böck (1993) The product of hypB gene, which is required for nickel incorporation into hydrogenases, is a novel guanine nucleotide-binding protein. J. Bacteriol. 175, 630-635.
8. [8] Colbeau, A., Richaud, P., Toussaint, B., Caballero, F.J., Elster, C., Delphin, C., Smith, R.L., Chabert, J. and Vignais, P.M. (1993) Organization of the genes necessary for hydrogenase expression in Rhodobacter capsulatus. Sequence analysis and identification of two hyp regulatory mutants. Mol. Microbiol. 8, 15-29.
9. [9] Carrasco, C.D., Buettner, J.A. and Golden, J.W. (1995) Programmed DNA rearrangement of a cyanobacterial hupL gene in heterocysts. Proc. Natl. Acad. Sci. USA 92, 791-795.
10. [10] Tibelius, K.H., Du, L., Tito, D. and Stejskal, F. (1993) The Azotobacter chroococcum hydrogenase gene cluster: sequences and genetic analysis of four accessory genes, hupA, hupB, hupY and hupC. Gene 127, 53-61.
11. [11] Lutz, S., Jacobi, A., Schlensog, V., Böhm, R., Sawers, G. and A. Bock, A. (1991) Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli. Mol. Microbiol. 5, 123-135.
12. [12] Rey, L., Murillo, J., Hernando, Y., Hidalgo, E., Cabrera, E., Imperial, J. and Ruiz-Argüeso, T. (1993) Molecular analysis of a microaerobically induced operon required for hydrogenase synthesis in Rhizobium leguminosarum biovar viciae. Mol. Microbiol. 8, 471-481.
13. [13] Fu, C., Olson, J.W. and Maier, R.J. (1995) HypB protein of Bradyrhizobium japonicum is a metal-binding GTPase capable of binding 18 divalent nickel ions per dimer. Proc. Natl. Acad. Sci. USA 92, 2333-2337.
14. [14] Chen, J.C. and Mortenson, L.E. (1992) Identification of six open reading frames from a region of the Azotobacter vinelandii genome likely involved in dihydrogen metabolism. Biochim. Biophys. Acta 1131, 199-202.
15. [15] Borthakur, D. and Haselkorn, R. (1989) Tn5 mutagenesis of Anabaena sp. strain PCC7120: isolation of a new mutant unable to grow without combined nitrogen. J. Bacteriol. 10, 5759-5761.
16. [16] Amaravadi, L. and King, M.W. (1994) A rapid and efficient non-radioactive method for screening recombinant DNA libraries. BioTechniques 16, 98-103.
17. [17] Altschul, S.F., Gish, W., Miller, W., Myers, W.E. and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410.