Non-radiometric assay for glucosylglycerol-synthesizing enzymes involved in the cyanobacterial salt adaptation

Journal of Microbiological Methods

Volumn 27, Issue 2-3, 1996, Pages 139-145

  Schoor, Arne ^a   Hagemann, Martin ^a   Erdmann, Norbert ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Enzyme assay; Glucosylglycerol phosphate synthase phosphatase; HPLC; Osmolytes; Synechocystis

Indexed keywords

GLUCOSYLGLYCEROL; PHOSPHATASE; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ADAPTATION; ARTICLE; CONTROLLED STUDY; CYANOBACTERIUM; ENZYME ACTIVITY; ENZYME ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL;

EID: 0030292309     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(96)00940-2     Document Type: Article

References (23)

1. [1] Borowitzka, L.J., Demmerle, S., Mackay, M.A. and Norton, R.S. (1980) Carbon-13 nuclear magnetic resonance study of osmoregulation in a blue-green algae. Science 210, 650-651.
2. [2] Erdmann, N. (1983) Organic osmoregulatory solutes in blue-green algae. Z. Pflanzenphysiol. 110, 147-155.
3. [3] Reed, R.H., Borowitzka, L.J., Mackay, M.A., Chudek, J.A., Forster, R., Warr, S.R.C., Moore, D.J. and Stewart, W.D.P. (1986) Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiol. Rev. 39, 51-56.
4. [4] Galinski, E.A. and Trüper, H.G. (1994) Microbial behaviour in salt-stressed ecosystems. FEMS Microbiol. Rev. 15, 95-108.
5. [5] Warr, S.R.C., Reed, R.H., and Stewart, W.D.P. (1988) The compatibility of osmotica in cyanobacteria. Plant Cell Environ. 11, 137-142
6. [6] Kaneda, M., Mizutani, K. and Tanaka, K. (1982) Liloside C, a glycerol glucoside from Lilium lancifolium. Phytochemistry 21, 891-893.
7. [7] Bianchi, G., Gamba, A., Limiroli, R., Pozzi, N., Elster, R., Salamini, F. and Bartels, D. (1993) The unusual sugar composition in leaves of the resurrection plant Myrothamnus flabellifolia. Physiol. Plant. 87, 223-226.
8. [8] Pocard, J.-A., Smith, L.T., Smith, G.M. and Le Rudulier, D. (1994) A prominent role for glucosylglycerol in the adaptation of Pseudomonas mendocina SKB70 to osmotic stress. (1994) J. Bacteriol. 176, 6877-6884.
9. [9] Hagemann, M. and Erdmann, N. (1994) Activation and pathway of glucosylglycerol synthesis in the cyanobacterium Synechocystis sp. PCC 6803. Microbiol. 140, 1427-1431.
10. [10] Kauss, H., Thomson, K.S., Tetour, M. and Jeblick, W. (1978) Proteolytic activation of a galactosyl transferase involved in osmotic regulation. Plant Physiol. 61, 35-37.
11. [11] Rausch, U., Ahmad, H., Maier, G. and Kauss, H. (1991) Proteinase nature of an enzyme capable of activating the galactosyltransferase involved in volume regulation of Proterioochromonas malhamensis. Physiol. Plant. 82, 93-98.
12. [12] Kremer, B.P. and Kirst, G.O. (1981) Biosynthesis of 2-ο-D-glycerol-α-D-galactopyranoside (floridoside) in marine Rhodophyceae. Plant Sci. Lett. 23, 349-357.
13. [13] Paschoalin, V.M.F., Silva, J.T. and Panek, A.D. (1989) Identification of an ADPG-dependent trehalose synthase in Saccharomyces. Curr. Genet. 16, 81-87.
14. [14] Araujo, P.S., Panek, A.C., Ferreira, R. and Panek, A.D. (1989) Determination of trehalose in biological samples by a simple and stable trehalase preparation. Anal. Biochem. 176, 432-436.
15. [15] Salvucci, M.E. and Crafts-Brander, S.J. (1991) A high-performance liquid chromatography based radiometric assay for sucrose-phosphate synthase and other UDP-glucose requiring enzymes. Anal. Biochem. 194, 365-368.
16. [16] Operti, M.S., Oliveira, D.E., Freitas-Valle, A.B., Oestreicher, E.G., Mattoon, J.R. and Panek, A.D. (1982) Relationships between trehalose and maltose utilization in Saccharomyces cerevisiae. Curr. Genet. 5, 69-76.
17. [17] Londesborough, J. and Vuorio, O. (1991) Trehalose-6-phosphate synthase/phosphatase complex from baker's yeast: purification of a proteolytically activated form. J. Gen. Microbiol. 137, 323-330.
18. [18] Petit, T. and François, J. (1994) Accumulation of trehalose in Saccharomyces cerevisiae growing on maltose is dependent on the TPS1 gene encoding UDPglucose-linked trehalose synthase. FEBS Lett. 355, 309-313.
19. [19] Giæver, H.M., Styrvold, O.B., Kaasen, I. and Strøm, A.R. (1988) Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli. J. Bacteriol. 170, 2841-2849.
20. [20] Allen, M.B. and Arnon, D.J. (1955) Studies on nitrogen-fixing algae. II. The sodium requirement of Anabaena cylindrica. Physiol. Plant. 8, 653-660.
21. [21] Bergmeyer, H.U. (1988) Methods in Enzymatic Analysis. Verlag Chemie, Weinheim, Germany.
22. [22] Schoor, A., Erdmann, N., Effmert, U. and Mikkat, S. (1995) Determination of the cyanobacterial osmolyte glucosylglycerol by high-perfomance liquid chromatography. J. Chromatogr. 704, 89-97.
23. [23] Bradford, M.A. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.