Qnantification of hepatitis C virus RNA in peripheral blood mononuclear cells: A comparison between patients chronically infected by HCV and patients coinfected by HIV

Research in Virology

Volumn 147, Issue 6, 1996, Pages 325-332

  Cribier, B ^a   Rey, D ^b   Uhl, G ^b   Schmitt, C ^a   Libbrecht, E ^b   Vetter, D ^b   Lang, J M ^b   Kirn, A ^a   Stoll Keller, F ^a  

^a INSERM   (France)

^b UNIVERSITY HOSPITAL OF STRASBOURG   (France)

Author keywords

Branched DNA assay; HCV; HIV; PBMC; RT PCR

Indexed keywords

VIRUS RNA;

ADULT; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MALE; MONONUCLEAR CELL; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIREMIA; VIRUS REPLICATION; CHRONIC DISEASE; GENETICS; GENOTYPE; ISOLATION AND PURIFICATION; MIDDLE AGED; POLYMERASE CHAIN REACTION; VIROLOGY;

HEPATITIS C VIRUS; HUMAN IMMUNODEFICIENCY VIRUS;

ADULT; CHRONIC DISEASE; FEMALE; GENOTYPE; HEPACIVIRUS; HEPATITIS C; HIV INFECTIONS; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; RNA, VIRAL; VIREMIA; VIRUS REPLICATION;

EID: 0030277191     PISSN: 09232516     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2516(97)85124-2     Document Type: Article

References (25)

1. Bartolomé, J., Castillo, I., Quiroga, J.S., Navas, S. & Carreno, V. (1993), Detection of hepatitis C virus RNA in serum and peripheral blood mononuclear cells. J. Hepatol., 17, S90-S93.
2. Bielefeldt Ohmann, H., Ronsholt, L. & Bloch, B. (1987), Demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle. J. Gen. Virol., 68, 1971-1982.
3. Bouffard, P., Hayashi, P.H., Acevedo, R., Levy, N. & Zeldis, J.B. (1992), Hepatitis C virus is detected in a monocyte/macrophage subpopulation of peripheral blood mononuclear cells of infected patients. J. Infect. Dis., 166, 1276-1280.
4. Chomczynski, P. & Sacchi, N. (1987), Single step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal. Biochem., 162, 156-159.
5. Cribier, B., Schmitt, C., Bingen, A., Kirn, A. & Keller, F. (1995a), In vitro infection of peripheral blood mononuclear cells by hepatitis C virus. J. Gen. Virol., 76, 2485-2491.
6. Cribier, B., Rey, D., Schmitt, C., Lang, J.M., Kirn, A. & Keller, F. (1995b), High hepatitis C viremia and impaired antibody response in patients coinfected by human immunodeficiency virus. AIDS, 9, 1131-1136.
7. Eyster, M.E., Fried, M.W., Di Bisceglie, A.M. & Geodert, J.J. (1994), Increasing hepatitis C virus RNA levels in haemophiliacs: relationship to human immunodeficiency virus infection and liver disease. Blood, 84, 1020-1023.
8. Ferri, C., Monti, M., La Civita, L., Longonbardo, G., Greco, F., Pasero, G., Gentilini, P., Bombardieri, S. & Zignego, A.L. (1993), Infection of peripheral blood mononuclear cells by hepatitis C virus in mixed cryoglobulinemia. Blood, 82, 3701-3704.
9. Inchauspe, G., Abe, K., Zebedee, S., Nasoff, M. & Prince, A.M. (1991), Use of conserved sequences from hepatitis C virus for the detection of the viral RNA in infected sera by the polymerase chain reaction. Hepatology, 14, 595-600.
10. Hellings, J.A., Van Der Veen-Du Prie, J., Snelting-Van Densen, R. & Stude, R. (1985), Preliminary results of transmission experiments of non-A,non-B hepatitis by mononuclear leukocytes from a chronic patient. J. Virol. Methods, 10, 321-326.
11. Kaito, M., Watanabe, S., Tsukiyama-Kohara, K., Yamagushi, K., Kobayashi, Y., Koshini, M., Yokoi, M., Ishida, S., Suzuki, S. & Kohara, M. (1994), Hepatitis C virus particle detected by immunoelectron microscopic study. J. Gen. Virol., 75, 1755-1760.
12. Kuasaka, S., Okusa, T., Araki, A., Fujiki, K., Takashimizu, I., Okayasu, I., Yamamoto, I. & Sato, C. (1995), Prediction of relapses after interferon-alpha therapy by hepatitis C virus RNA in peripheral blood mononuclear cells. J. Med. Virol., 46, 265-268.
13. Kuo, G., Choo, Q.L., Alter, H.J., Gitnick, L., Redecker, A.G., Purcell, R.H., Miyamura, T., Dienstag, J.L., Alter, M.J., Stevens, C.E., Tegmeier, G.E., Bonino, F., Colombo, M., Lee, W.S., Kuo, C., Berger, K., Sauster, J.K., Overby, L.R., Bradley, D.W. & Houghton, M. (1989), An assay for circulating antibodies to a major etiologic virus of non-A,non-B hepatitis. Science, 244, 362-364.
14. Lamelin, J.P., Zoulim, F. & Trépo, C. (1995), Lymphotropism of hepatitis B and C viruses: an update and a newcomer. Int. J. Clin. Lab. Res., 25, 1-6.
15. Miller, R.H. & Purcell, R.H. (1990), Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proc. Natl. Acad. Sci. USA, 87, 2057-2061.
16. Lau, J.Y.N., Simmonds, P. & Urdea, M.S. (1995) Implications of variations of "conserved" regions of hepatitis C virus genome. Lancet, 346, 425-426.
17. Lerat, H., Berby, F., Trabaud, A.M., Viladin, O., Major, M., Trépo, C. & Inchauspé, G. (1996), Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells. J. Clin. Invest., 97, 845-851.
18. Mihm, S., Hartmann, H. & Ramadori, G. (1996) A reevaluation of the association of hepatitis C virus replicative intermediates with peripheral blood cells including granulocytes by a tagged reverse transcription/polymerase chain reaction technique. J. Hepatol., 24, 491-497.
19. Moldvay, J., Deny, P., Pol, S., Bréchot, C. & Lamas, E. (1994), Detection of hepatitis C virus RNA in peripheral blood mononuclear cells of infected patients by in situ hybridization. Blood, 83, 269-273.
20. Muller, H.M., Pfaff, E., Goeser, T., Kallinowski, B., Solbach, C. & Theilman L. (1993), Peripheral blood mononuclear leukocytes serve as possible extrahepatic site for hepatis C virus. J. Gen. Virol., 74, 669-676.
21. Muratori, L., Gisotra, Cataleta, M., Francesconi, R., Ballardini, G., Cassani, F., Lenzi, M. & Bianchi, F.B. (1994), Testing for hepatitis C virus sequences in peripheral blood mononuclear cells of patients with chronic hepatitis C in the absence of hepatitis C virus RNA. Liver, 14, 124-128.
22. Shimizu, Y.K., Iwamoto, A., Hijikata, M., Purcell, R.H. & Yoshikuba, H. (1992), Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proc. Natl. Acad. Sci., USA, 90, 6037-6041.
23. Telfer, P.T., Brown, D., Devereux, H., Lee, C.A. & Du Sheiko, G.M. (1994), HCV RNA levels and HIV infection: evidence for a viral interaction in haemophilic patients. Br. J. Haematol., 88, 397-399.
24. Willems, M., Peerlinck, K., Moshage, H., Deleu, I., Van Den Eynden, C., Vermylen, J. & Yap, S.H. (1994), Hepatitis C virus RNAs in plasma and in peripheral blood mononuclear cells of hemophiliacs with chronic hepatitis: evidence for viral replication in peripheral blood mononuclear cells. J. Med. Virol., 42, 272-278.
25. Zignego, A.L., Macchia, D., Monti, M., Thiers, V., Mazetti, M., Foschi, M., Maggi, E., Romagnani, S., Gentilini, P. & Bréchot, C. (1992), Infection of peripheral blood mononuclear cells by hepatitis C virus. J. Hepatol., 15, 382-386.