Measurement of glycine receptor function by radioactive chloride uptake

Journal of Neuroscience Methods

Volumn 68, Issue 2, 1996, Pages 253-257

  Blednov, Yuri A ^a,c   Bleck, Virginia ^a   Harris, R Adron ^a,b  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF COLORADO   (United States)

^c INSTITUTE OF PHARMACOLOGY   (Russian Federation)

Author keywords

Chloride channel; Chloride flux; Glycine; Mouse spinal cord; Picrotoxin; Strychnine

Indexed keywords

BICUCULLINE; GLYCINE; GLYCINE RECEPTOR; PENTOBARBITAL; PICROTOXIN; STRYCHNINE;

ANIMAL TISSUE; ARTICLE; BRAIN STEM; CELL CULTURE; CHLORIDE TRANSPORT; CONTROLLED STUDY; ELECTROPHYSIOLOGY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPINAL CORD;

EID: 0030273551     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-0270(96)00088-X     Document Type: Article

References (19)

1. Allan, A.M. and Harris, R.A. (1986) γ-aminobutyric acid agonists and antagonists alter chloride flux across brain membranes, Mol. Pharmacol., 29: 497-505.
2. Betz, H. (1991) Glycine receptors: Heterogeneous and widespread in the mammalian brain, Trends Neurosci., 14: 458-461.
3. Cash, D.J. and Subarrao, K. (1987) Channel opening of γ-aminobutyric acid receptor from rat brain: Molecular mechanisms of the receptor responses, Biochemistry. 26: 7562-7570.
4. - fluxes in rat brain synaptoneurosomes, J. Neurochem., 57: 384-390.
5. García-Calvo, M., Valdivieso, F., Mayor, F., Jr. and Vázquez, J. (1992) Sensitive procedures for measuring chloride fluxes mediated by the purified glycine receptor incorporated into phospholipid vesicles. Neurosci. Lett., 136: 102-104.
6. Gundlach, A.L., Kortz, G., Burazin, T.C., Madigan, J. and Higgins, R.J. (1993) Deficit of inhibitory glycine receptors in spinal cord from Peruvian Pasos: Evidence for an equine form of inherited myclonus, Brain Res., 628: 263-270.
7. Harris, R.A. and Allan, A.M. (1985) Functional coupling of GABA receptors to chloride channels in brain membranes. Science, 228: 1108-1110.
8. Harris, R.A. and Allan, A.M. (1989) Alcohol intoxication: Ion channels and genetics. FASEB J., 3: 1689-1695.
9. Harris, R.A., McQuilkin, S.J., Paylor, R., Abeliovich, A., Tonegawa, S. and Wehner, J.M. (1995) Mutant mice lacking the γ isoform of protein kinase C show decreased behavioral actions of ethanol and altered function of γ-aminobutyrate type A receptors, Proc. Natl. Acad. Sci. USA, 92: 3658-3662.
10. Hollingsworth, E.B., McNeal, E.T., Burton, J.L., Williams, R.J., Daly, J.W. and Creveling, C.R. (1985) Biochemical characterization of a filtered synaptoneurosome preparation from guinea pig cerebral cortex: Cyclic adenosine 3′5′-monophosphate-generating systems, receptors, enzymes, J. Neurosci., 5: 2240-2253.
11. Kuhse, J., Schmieden, V. and Betz, H. (1990) A single amino acid exchange alters the pharmacology of neonatal rat glycine receptor subunit. Neuron, 5: 867-873.
12. Langosch, D., Becker, C.-M. and Betz, H. (1990) The inhibitory glycine receptor: A ligand-gated chloride channel of the central nervous system, Eur. J. Biochem., 194: 1-8.
13. Pribilla, I., Takagi, T., Langosch, D., Bormann, J. and Betz, H. (1992) The atypical M2 segment of the β subunit confers picrotoxin in resistance to inhibitory glycine receptor channels, EMBO J., 11: 4305-4311.
14. Rajendra, S., Lynch, J.W., Pierce, K.D., French, C.R., Barry, P.H. and Schofield, P.R. (1994) Startle disease mutations reduce the agonist sensitivity of the human inhibitory glycine receptor. J. Biol. Chem., 269: 18739-18742.
15. Rajendra, S. and Schofield, P.R. (1995) Molecular mechanisms of inherited startle syndromes. Trends Neurosci., 18: 80-82.
16. Saitoh, T., Ishida, M., Maruyama, M. and Shinozaki, H. (1994) A novel antagonist, phenylbenzene ω-phosphono-α-amino acid, for strychnine-sensitive glycine receptors in the rat spinal cord, Br. J. Pharmacol., 113: 165-170.
17. 2 glycine receptors. FEBS Lett., 303: 17S-180.
18. Walstrom, K.M. and Hess, G.P. (1994) Mechanism for the channel-opening reaction of strychnine-sensitive glycine receptors on cultured embryonic mouse spinal cord cells, Biochemistry. 33: 7718-7730.
19. Young, A.B. and Snyder, S.H. (1974) Strychnine binding in rat spinal cord membranes associated with the synaptic glycine receptor: Cooperativity of glycine interactions, Mol. Pharm., 10: 790-809.