Quantitation of feline immunodeficiency proviruses in doubly infected cats using competitive PCR and a fluorescence-based RFLP

Journal of Virological Methods

Volumn 62, Issue 1, 1996, Pages 21-31

  Cammarota, Giancarlo ^a   Da Prato, Luigi ^a   Nicoletti, Elisa ^a   Matteucci, Donatella ^a   Bendinelli, Mauro ^a   Pistello, Mauro ^a  

^a UNIVERSITY OF PISA   (Italy)

Author keywords

FIV; PCR; RFLP

Indexed keywords

VIRUS VACCINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CAT; CONTROLLED STUDY; FELINE IMMUNODEFICIENCY VIRUS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; VIRUS DETECTION; VIRUS INFECTION;

ANIMALIA; FELIDAE; FELINE IMMUNODEFICIENCY VIRUS; FELIS CATUS;

EID: 0030273163     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(96)02085-X     Document Type: Article

References (14)

1. Bandecchi, P., Pistello, M., Matteucci, D., Lombardi, S., Bendinelli, M. and Tozzini, F. (1995) Examination of variables affecting syncytium formation by, and serum neutralization of, feline immunodeficiency virus on CrFK cells. Microbiologica 18, 1-12.
2. Bendinelli, M., Pistello, M., Lombardi, S. et al. (1995) Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen. Clin. Microbiol. Rev. 8, 87-112.
3. Cammarota, G., Nicoletti, E., Matteucci, D. et al. (1996) Genotyping of 16 FIV isolates based on the gag nucleotide sequence. Third International Feline Retrovirus Research Symposium, Fort Collins, CO, USA.
4. Gelman, I.H., Zhang, J., Hailman, E., Hanafusa, H. and Morse, S.S. (1992) Identification and evaluation of new primer sets for the detection of lentivirus proviral DNA. AIDS Res. and Hum. Retroviruses 8, 1981-1989.
5. Giannecchini S., Matteucci D., Mazzetti, P. and Bendinelli, M. Incubation time for feline immunodeficiency virus cultures. J. Clin. Microbiol., in press.
6. Lowagie, J., McCutchan, F.E., Peeters, M. et al. (1993) Comparison of gag genes from seventy international HIV-1 isolates provides evidence for multiple genotic subtypes. AIDS 7, 769-780.
7. Matteucci, D., Baldinotti, F., Mazzetti, P. et al. (1993) Detection of feline immunodeficiency virus in saliva and plasma by cultivation and polymerase chain reaction. J. Clin. Microbiol. 31, 494-451.
8. Matteucci, D., Mazzetti, P., Baldinotti, F., Zaccaro, L. and Bendinelli, M. (1995) The feline lymphoid cell line MBM and its use for feline immunodeficiency virus isolation and quantitation. Vet. Immunol. Immunopathol. 43, 71-82.
9. Matteucci, D., Pistello, M., Mazzetti, P. et al. (1996) Vaccination protects against in vivo-grown feline immunodeficiency virus even in the absence of detectable neutralizing antibodies. J. Virol. 70, 617-622.
10. Pistello, M., Menzo S., Giorgi, M. et al. (1994) Competitive polymerase chain reaction for quantitating feline immunodeficency virus load in infected cat tissues. Mol. Cell. Probes 8, 229-234.
11. Pistello, M., Matteucci, D., Cammarota, G. et al. (1996) Pre-infection with a poorly pathogenic FIV does not prevent infection by a fully virulent heterologous strain. Third International Feline Retrovirus Research Symposium, Fort Collins, CO, USA.
12. Sodora, D.L., Shpaer, E.G., Kitchell, B.E., Dow, S.W., Hoover, E.A. and Mullins, J.I. (1994) Identification of three feline immunodeficiency virus (FIV) env gene subtypes and comparison of the FIV and human immunodeficiency virus type 1 evolutionary patterns. J. Virol. 68, 2230-2238.
13. Talbott, R.L., Sparger, E.E., Lovelace, K.M. et al. (1989) Nucleotide sequence and genomic organization of feline immunodeficiency virus. Proc. Natl. Acad. Sci. USA, 86, 5743-5747.
14. Willett, B.J. and Jarrett, O. (Eds.,) (1995) Feline Immunology and Immunodeficiency, Oxford University Press, Oxford.