-
4
-
-
0011872872
-
-
J.N. Abelson, Simon M.I. New York: Academic Press Inc. Methods of Enzymology
-
Goeddel DV. Abelson JN, Simon MI. Gene Expression Technology. 185:1990;Academic Press Inc, New York. Methods of Enzymology.
-
(1990)
Gene Expression Technology
, vol.185
-
-
Goeddel, D.V.1
-
6
-
-
0027932534
-
Engineered biosynthesis of a complete macrolactone in a heterologous host
-
Kao CM, Katz L, Khosla C. Engineered biosynthesis of a complete macrolactone in a heterologous host. Science. 265:1994;509-512.
-
(1994)
Science
, vol.265
, pp. 509-512
-
-
Kao, C.M.1
Katz, L.2
Khosla, C.3
-
7
-
-
0029154137
-
Erythromycin biosynthesis. Highly efficient incorporation of polyketide chain elongation intermediates into 6-deoxyerythronolide B in an engineered Streptomyces host
-
Cane DE, Luo G, Khosla C, Kao CM, Katz L. Erythromycin biosynthesis. Highly efficient incorporation of polyketide chain elongation intermediates into 6-deoxyerythronolide B in an engineered Streptomyces host. J Antibiot (Tokyo). 48:1995;647-651.
-
(1995)
J Antibiot (Tokyo)
, vol.48
, pp. 647-651
-
-
Cane, D.E.1
Luo, G.2
Khosla, C.3
Kao, C.M.4
Katz, L.5
-
8
-
-
0028784464
-
Cell-free synthesis of polyketides by recombinant erythromycin polyketide synthases
-
Pieper R, Luo G, Cane DE, Khosla C. Cell-free synthesis of polyketides by recombinant erythromycin polyketide synthases. Nature. 378:1995;263-266.
-
(1995)
Nature
, vol.378
, pp. 263-266
-
-
Pieper, R.1
Luo, G.2
Cane, D.E.3
Khosla, C.4
-
9
-
-
0029161160
-
Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2)
-
Bedford DJ, Schweizer E, Hopwood DA, Khosla C. Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2). J Bacteriol. 177:1995;4544-4548.
-
(1995)
J Bacteriol
, vol.177
, pp. 4544-4548
-
-
Bedford, D.J.1
Schweizer, E.2
Hopwood, D.A.3
Khosla, C.4
-
10
-
-
0029039064
-
Production of the new antibiotic tetrahydrokalafungin by transformants of the kalafungin producer Streptomyces tanashiensis
-
Kakinuma S, Ikeda H, Takada Y, Tanaka H, Hopwood DA, Omura S. Production of the new antibiotic tetrahydrokalafungin by transformants of the kalafungin producer Streptomyces tanashiensis. J Antibiot (Tokyo). 48:1995;484-487.
-
(1995)
J Antibiot (Tokyo)
, vol.48
, pp. 484-487
-
-
Kakinuma, S.1
Ikeda, H.2
Takada, Y.3
Tanaka, H.4
Hopwood, D.A.5
Omura, S.6
-
11
-
-
0029027352
-
Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage
-
of special interest. The 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea, which synthesizes the aglycone core of the antibiotic erythromycin A, has been modified by the repositioning of a chain-terminating cyclase domain to the carboxyl terminus of 6-deoxyerythronolide B synthase. The resulting mutant markedly accelerates the formation of the predicted triketide lactone, compared to a control in which the repositioned domains is inactive. The repositioning the the cyclase should be generally useful for redirecting polyketide synthesis to obtain polyketide of specified chain lengths.
-
Cortes J, Wiesman KE, Roberts GA, Brown MJ, Staunton J, Leadlay PF. Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage. of special interest Science. 268:1995;1487-1489 The 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea, which synthesizes the aglycone core of the antibiotic erythromycin A, has been modified by the repositioning of a chain-terminating cyclase domain to the carboxyl terminus of 6-deoxyerythronolide B synthase. The resulting mutant markedly accelerates the formation of the predicted triketide lactone, compared to a control in which the repositioned domains is inactive. The repositioning the the cyclase should be generally useful for redirecting polyketide synthesis to obtain polyketide of specified chain lengths.
-
(1995)
Science
, vol.268
, pp. 1487-1489
-
-
Cortes, J.1
Wiesman, K.E.2
Roberts, G.A.3
Brown, M.J.4
Staunton, J.5
Leadlay, P.F.6
-
12
-
-
0029064652
-
Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits
-
of outstanding interest. The concept of rational design is illustrated by the preparation of Streptomyces strains that produce two new polyketides by the expression of combinations of appropriate enzymatic subunits from naturally occurring polyketide synthases.
-
McDaniel R, Ebert-Khosla S, Hopwood DA, Khosla C. Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits. of outstanding interest Nature. 375:1995;549-554 The concept of rational design is illustrated by the preparation of Streptomyces strains that produce two new polyketides by the expression of combinations of appropriate enzymatic subunits from naturally occurring polyketide synthases.
-
(1995)
Nature
, vol.375
, pp. 549-554
-
-
McDaniel, R.1
Ebert-Khosla, S.2
Hopwood, D.A.3
Khosla, C.4
-
13
-
-
0029670331
-
Engineered biosynthesis of novel polyketides: Properties of the whiE aromatase/cyclase
-
Alvarez MA, Fu H, Khosla C, Hopwood DA, Bailey JE. Engineered biosynthesis of novel polyketides: properties of the whiE aromatase/cyclase. Nat Biotechnol. 14:1996;335-338.
-
(1996)
Nat Biotechnol
, vol.14
, pp. 335-338
-
-
Alvarez, M.A.1
Fu, H.2
Khosla, C.3
Hopwood, D.A.4
Bailey, J.E.5
-
14
-
-
0026415106
-
Modular organization of genes required for complex polyketide biosynthesis
-
Donadio S, Staver MJ, McAlpine JB, Swanson SJ, Katz L. Modular organization of genes required for complex polyketide biosynthesis. Science. 252:1995;675-679.
-
(1995)
Science
, vol.252
, pp. 675-679
-
-
Donadio, S.1
Staver, M.J.2
McAlpine, J.B.3
Swanson, S.J.4
Katz, L.5
-
15
-
-
0026629738
-
Biosynthesis of erythromycin macrolactone and a rational approach for producing hybrid macrolides
-
Donadio S, Staver MJ, McAlpine JB, Swanson SJ, Katz L. Biosynthesis of erythromycin macrolactone and a rational approach for producing hybrid macrolides. Gene. 115:1992;97-103.
-
(1992)
Gene
, vol.115
, pp. 97-103
-
-
Donadio, S.1
Staver, M.J.2
McAlpine, J.B.3
Swanson, S.J.4
Katz, L.5
-
16
-
-
0027234764
-
An erythromycin analog produced by reprogramming of polyketide synthesis
-
Donadio S, McAlpine JB, Sheldon PJ, Jackson M, Katz L. An erythromycin analog produced by reprogramming of polyketide synthesis. Proc Natl Acad Sci USA. 90:1993;7119-7123.
-
(1993)
Proc Natl Acad Sci USA
, vol.90
, pp. 7119-7123
-
-
Donadio, S.1
McAlpine, J.B.2
Sheldon, P.J.3
Jackson, M.4
Katz, L.5
-
17
-
-
0027455631
-
Identification of a Saccharopolyspora gene required for the final step in erythromycin biosynthesis
-
Stassi D, Donadio S, Staver MJ. Identification of a Saccharopolyspora gene required for the final step in erythromycin biosynthesis. J Bacteriol. 175:1993;182-189.
-
(1993)
J Bacteriol
, vol.175
, pp. 182-189
-
-
Stassi, D.1
Donadio, S.2
Staver, M.J.3
|