High-resolution NMR of biological solids

Current Opinion in Structural Biology

Volumn 6, Issue 5, 1996, Pages 624-629

  McDowell, Lynda M ^a   Schaefer, Jacob ^a  

^a WASHINGTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLOID; CARBON 13; DEUTERIUM;

BETA CHAIN; BINDING SITE; GEOMETRY; LIGAND BINDING; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN SECONDARY STRUCTURE; SHORT SURVEY; SOLID;

EID: 0030272669     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-440X(96)80028-5     Document Type: Article

References (59)

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54. 1H NMR of nearly three orders of magnitude are possible in principle. One practical problem is that the electron-nuclear transfer is slow (seconds) and leakage via nuclear spin-lattice relaxation usually eliminates most of the sensitivity enhancement. By performing the transfer at 14 K, however, the leakage was slowed and about one third of the theoretical enhancement was observed for glycine frozen in a 40:60 water : glycerol mixture doped by 50 mM nitroxide free radical. The technical problems yet to be overcome for biological applications include making magic-angle spinning at 14K routine and establishing that the required high concentrations of dopant are compatible with unperturbed protein structure.
55. 13C resonances.
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