Genotyping of Helicobacter pylori isolates by sequencing of PCR products and comparison with the RAPD technique

Research in Microbiology

Volumn 147, Issue 8, 1996, Pages 661-669

  Kansau, I ^a,d   Raymond, J ^b   Bingen, E ^c   Courcoux, P ^a   Kalach, N ^b   Bergeret, M ^b   Braimi, N ^c   Dupont, C ^b   Labigne, A ^a  

^a INSERM   (France)

^b HÔPITAL SAINT VINCENT DE PAUL   (France)

^c HÔPITAL ROBERT DEBRÉ   (France)

^d Universidad De Los Andes Facultad de Medicina   (Venezuela)

Author keywords

comparison; genotyping; Helicobacter pylori; PCR; RAPD; sequencing

Indexed keywords

ADOLESCENT; ADULT; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA SEQUENCE; FEMALE; GENOTYPE; HELICOBACTER PYLORI; HUMAN; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI;

EID: 0030271786     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/0923-2508(96)84023-X     Document Type: Article

References (21)

1. Akopyantz, N., Bukanov, N., Westblom, T., Kresovich, S. & Berg, D. (1992), DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic. Acids Res., 20, 5137-5142.
2. Bingen, E., Boissinot, C., Desjardins, P., Cave, H., Brahimi, N., Lambert-Zechovsky, N., Denamur, E., Blot, P. & Elion, J. (1993) Arbitrary primed polymerase chain reaction provides rapid differentiation of Proteus mirabilis isolates from a pediatric hospital. J. Clin. Microbiol., 31, 1055-1059.
3. Blaser, M. (1992), Helicobacter pylori : its role in disease. Clin. Infect. Dis., 15, 386-393.
4. Clayton, C., Kleanthous, H., Morgan, D., Puckey, L. & Tabaqchali, S. (1993), Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and restriction fragment length polymorphism analysis. J. Clin. Microbiol., 31, 1420-1425.
5. Desai, M., Linton, D., Owen, R.J. & Stanley, J. (1994), Molecular typing of Helicobacter pylori isolates from asymptomatic, ulcer and gastritis patients by urease gene polymorphism. Epidemiol. Infect., 112, 151-160.
6. Evans, D.G., Evans, D.J., Jr, Lampert, H.C. & Graham, D.Y. (1995), Restriction fragment lenght polymorphism in the adhesin gene hpaA of Helicobacter pylori. Am. J. Gastroenterol., 90, 1282-1288.
7. Fujimoto, S., Marshall, B. & Blaser, M.J. (1994), PCR-based restriction fragment length polymorphism typing of Helicobacter pylori. J. Clin. Microbiol., 32, 331-334.
8. Kansau, I. & Labigne, A. (1995), The HspA antigen is highly conserved among Helicobacter pylori clinical isolates and its use in serological and protection studies is warranted (Abstract). Gut, 37, A6.
9. Klein, P., Gastrointestinal Physiology Working Group, Graham, D.Y., Gaillour, A., Opekun, A. & Smith, E. (1991), Water source as risk factor for Helicobacter pylori infection in Peruvian children. Lancet, 337, 1503.
10. Labigne, A., Cussac, V. & Courcoux, P. (1991), Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity. J. Bacteriol., 173, 1920-1931.
11. Langenberg, W., Rauws, E., Widjojokusumo, A., Tytgat, G. & Zanen, H. (1986), Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J. Clin. Microbiol., 24, 414-417.
12. Marshall, D., Chua, A., Keeling, P., Sullivan, D., Coleman, D. & Smyth, C. (1995), Molecular analysis of Helicobacter pylori populations in antral biopsies from individual patients using randomly amplified polymorphic DNA (RAPD) fingerprinting. FEMS Immunol. Med. Microbiol., 10, 317-324.
13. Mégraud, F., Brassens-Rabbe, M.P., Denis, F., Belbovric, A. & Hoa, D.G. (1989), Seroepidemiology of Campylobacter pylori infection in various populations. J. Clin. Microbiol., 27, 1870-1873.
14. Mengin-Lecreulx, D. & Van Heijenoort, J. (1995), Characterization of the essential glmM gene encoding phosphoglucosamine mutase in Escherichia coli. J. Biol. Chem. 271, 32-39.
15. Meunier, J.R. & Grimont, P. (1993), Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol., 144, 373-379.
16. Morgan, D.D. & Owen, R.J. (1990), Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts. Mol. Cell. Probes, 4, 321-325.
17. Owen, R.J., Bickley, J., Hurtado, A., Fraser, A. & Pounder, R.E. (1994), Comparison of PCR-based restriction length polymorphism analysis of urease genes with rRNA gene profiling for monitoring Helicobacter pylori infections in patients on triple therapy. J. Clin. Microbiol., 32, 1203-1210.
18. Raymond, J., Begeret, M., Benhamou, P.H., Mensah, K. & Dupont, C. (1994), A 2-year study of H. pylori in children. J. Clin. Microbiol., 32, 461-463.
19. Robertson, B. & Labigne, A. (1995), Sequencing of PCR products, in "Helicobacter pylori: techniques for clinical diagnosis and basic research" (A. Lee & F. Mégraud) (pp. 146-151). W.B. Saunders Company, London.
20. Schutze, K., Hentschel, E., Dragosics, B. & Hirschl, A.M. (1995), Helicobacter pylori reinfection with identical organisms - transmission by the patients' spouses. Gut, 36, 831-833.
21. Taylor, N.S., Fox, J.G., Akopyants, N.S., Berg, D.E., Thompson, N., Shames, B., Yan, L., Fontham, E., Janney, F. Hunter, F.M. & Correa, P. (1995), Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fingerprinting. J. Clin. Microbiol., 33, 918-923.