Calcium influences the stability and conformation of rotavirus SA11 glycoprotein VP7 expressed in Dictyostelium discoideum

Journal of Biotechnology

Volumn 50, Issue 2-3, 1996, Pages 149-159

  Emslie, Kerry R ^a   Coukell, M Barrie ^a,b   Birch, Debra ^a   Williams, Keith L ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b YORK UNIVERSITY   (Canada)

Author keywords

Ca2+; Conformation; Dictyostelium; Protein expression; Rotavirus VP7

Indexed keywords

ANTIBODIES; CALCIUM; CHELATION; CONFORMATIONS; PRECIPITATION (CHEMICAL); VIRUSES;

CONFORMATION DEPENDENT NEUTRALIZING ANTIBODY; DICTYOSTELIUM DISCOIDEUM; IMMUNOBLOT ANALYSIS; IMMUNOPRECIPITATION; PROTEOLYSIS; ROTAVIRUS OUTER CAPSID GLYCOPROTEIN;

PROTEINS;

CALCIMYCIN; CALCIUM ION; CAPSID PROTEIN; EGTAZIC ACID; MONOCLONAL ANTIBODY; NEUTRALIZING ANTIBODY; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; VIRUS GLYCOPROTEIN;

ARTICLE; DICTYOSTELIUM DISCOIDEUM; GASTROENTERITIS; GENE EXPRESSION; IMMUNOBLOTTING; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; ROTAVIRUS; VIRUS CAPSID; VIRUS CELL INTERACTION; VIRUS INFECTION;

EID: 0030271221     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01557-X     Document Type: Article

References (23)

1. Baumann, O., Walz, B., Somlyo, A.V. and Somlyo, A.P. (1991). Electron probe microanalysis of calcium release and magnesium uptake by endoplasmic reticulum in bee photoreceptors. Proc. Natl. Acad. Sci. USA 88, 741-744.
2. Bellamy, A.R. and Both, G.W. (1990). Molecular biology of rotaviruses. Adv. Virus Res. 38, 1-43.
3. Bonner, J.T. (1947). Evidence for the formation of cell aggregates by chemotaxis in development of the slime mold Dictyostelium discoideum. J. Exp. Zool. 106, 1-26.
4. Cohen, J., Laporte, J., Charpilienne, A. and Scherrer, R. (1979). Activation of rotavirus RNA polymerase by calcium chelation. Arch. Virol. 60, 177-186.
5. Coukell, M.B. and Cameron, A.M. (1990). Calcium depletion of Dictyostelium cells selectively inhibits cyclic nucleotide phosphodiesterase synthesis a1 a post-transcriptional step. J. Cell Sci. 97, 649-657.
6. Coukell, M.B., Cameron, A.M. and Adames, N.R. (1992). Involvement of intracellular calcium in protein secretion in Dictyostelium discoideum. J. Cell Sci. 103, 371-380.
7. Dittrich, W., Williams, K.L. and Slade, M.B. (1994). Production and secretion of recombinant proteins in Dictyostelium discoideum. Bio/Technology 12, 614-617.
8. Dormitzer, P.R. and Greenberg, H.B. (1992). Calcium chelation induces a conformational change in recombinant herpes simplex virus-1-expressed rotavirus VP7. Virology 189, 828-832.
9. Emslie, K.R., Miller, J.M., Slade, M.B., Dormitzer, P.R., Greenberg, H.B. and Williams. K.L. (1995a). Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum. J. Virol. 69, 1747-1754.
10. Emslie, K.R., Slade, M.B., and Williams, K.L. (1995b). From virus to vaccine: developments using the simple eukaryote, Dictyostelium discoideum. Trends Microbiol. 3, 476-479.
11. Fabiato, A. and Fabiato, F. (1979). Calculator programs for computing the composition of the solution containing multiple metals and ligands used for experiments in skinned muscle cells. J. Physiol. (Paris) 75, 463-505.
12. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
13. Poruchynsky, M.S., Maass, D.R. and Atkinson, P.H. (1991). Calcium depletion blocks the maturation of rotavirus by altering the oligomerization of virus-encoded proteins in the ER. J. Cell Biol. 114, 651-661.
14. Shahrabadi, M.S. and Lee, P.W.K. (1986). Bovine rotavirus maturation is a calcium-dependent process. Virology 152, 298-307.
15. Shahrabadi, M.S., Babiuk, L.A. and Lee. P.W.K. (1987). Further analysis of the role of calcium in rotavirus morphogenesis. Virology 158, 103-111.
16. Shaw, R.D., Vo, P.T., Offit, P.A., Coulson, B.S. and Greenberg, H.B. (1986). Antigenic mapping of the surface proteins of rhesus rotavirus. Virology 155, 434-451.
17. Somlyo, A.P., Bond, M. and Somlyo, A.V. (1985). Calcium content of mitochondria and endoplasmic reticulum in liver frozen rapidly in vivo. Nature 314, 622-625.
18. Sussman, M. (1966). Biochemical and genetic methods in the study of cellular slime mold development. Methods Cell Physiol. 2, 397-440.
19. 2+ -dependent association with BiP. J. Cell Biol. 114, 189-205.
20. Svensson, L., Dormitzer, P.R., von Bonsdorff, C-H., Maunula, L. and Greenberg, H.B. (1994). Intracellular manipulation of disulphide bond formation in rotavirus proteins during assembly. J. Virol. 68, 5204-5215.
21. Watts, D.J. and Ashworth, J.M. (1970). Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem. J. 119, 171-174.
22. Wilczynska, Z. and Fisher. P.R. (1994). Analysis of a complex plasmid insertion in a phototaxis deficient transformant of Dictyostelium discoideum selected on a Micrococcus luteus lawn. Plasmid 32, 182-194.
23. Wileman, T., Kane, L.P., Carson, G.R. and Terhorst, C. (1991). Depletion of cellular calcium accelerates protein degradation in the endoplasmic reticulum. J. Biol. Chem. 266, 4500-4507.