메뉴 건너뛰기




Volumn 50, Issue 2-3, 1996, Pages 171-180

Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines: Large scale production of the E1 protein

Author keywords

Baculovirus; Bioreactor; Expression; Purification; Rubella virus; Spodoptera frugiperda; Trichoplusia ni

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMMONIUM COMPOUNDS; ANIMAL CELL CULTURE; BIOREACTORS; BIOSYNTHESIS; VIRUSES;

EID: 0030271183     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01562-3     Document Type: Article
Times cited : (8)

References (46)
  • 1
    • 0027285205 scopus 로고
    • Development of baculovirus triple and quadruple expression vectors: Co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells
    • Belyaev, A.S. and Roy, P. (1993) Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells. Nucleic Acids Res. 21, 1219-1223.
    • (1993) Nucleic Acids Res. , vol.21 , pp. 1219-1223
    • Belyaev, A.S.1    Roy, P.2
  • 2
    • 0023649612 scopus 로고
    • Nucleotide sequence and in vitro expression of rubella virus 24S subgenomic mRNA encoding the structural proteins E1, E2 and C
    • Clarke, D.M., Loo, T.W., Hui, I., Chong, P. and Gillam, S. (1987) Nucleotide sequence and in vitro expression of rubella virus 24S subgenomic mRNA encoding the structural proteins E1, E2 and C. Nucleic Acids Res. 15, 3041-3057.
    • (1987) Nucleic Acids Res. , vol.15 , pp. 3041-3057
    • Clarke, D.M.1    Loo, T.W.2    Hui, I.3    Chong, P.4    Gillam, S.5
  • 3
    • 77049142584 scopus 로고
    • A nomogram for ammonium sulphate solutions
    • Dixon, M. (1953) A nomogram for ammonium sulphate solutions. Biochem. J. 54, 457-458.
    • (1953) Biochem. J. , vol.54 , pp. 457-458
    • Dixon, M.1
  • 4
    • 0025287319 scopus 로고
    • Sequence of the genome of rubella virus: Evidence for genetic rearrangement during Togavirus evolution
    • Dommguez, G. Wang, C.-Y. and Frey, T.K. (1990) Sequence of the genome of rubella virus: evidence for genetic rearrangement during Togavirus evolution. Virology 177, 225-238.
    • (1990) Virology , vol.177 , pp. 225-238
    • Dommguez, G.1    Wang, C.-Y.2    Frey, T.K.3
  • 5
    • 0028890264 scopus 로고
    • Identification of rubella virus nonstructural proteins
    • Forng, R.-Y. and Frey, T.K. (1995) Identification of rubella virus nonstructural proteins. Virology 206, 843-853.
    • (1995) Virology , vol.206 , pp. 843-853
    • Forng, R.-Y.1    Frey, T.K.2
  • 6
    • 0028028506 scopus 로고
    • Molecular biology of rubella virus
    • Frey, T.K. (1994) Molecular biology of rubella virus. Adv. Virus Res. 44, 69-160.
    • (1994) Adv. Virus Res. , vol.44 , pp. 69-160
    • Frey, T.K.1
  • 7
    • 0024417243 scopus 로고
    • In vitro and in vivo expression of rubella virus glycoprotein E2: The signal peptide is contained in the C-terminal region of capsid protein
    • Hobman, T.C. and Gillam, S. (1989) In vitro and in vivo expression of rubella virus glycoprotein E2: the signal peptide is contained in the C-terminal region of capsid protein. Virology 173, 241-250.
    • (1989) Virology , vol.173 , pp. 241-250
    • Hobman, T.C.1    Gillam, S.2
  • 8
    • 0023696133 scopus 로고
    • Translocation of rubella virus glycoprolein E1 into the endoplasmic reticulum
    • Hobman, T.C., Shukin, R. and Gillam, S. (1988) Translocation of rubella virus glycoprolein E1 into the endoplasmic reticulum. J. Virol. 62, 4259-4264.
    • (1988) J. Virol. , vol.62 , pp. 4259-4264
    • Hobman, T.C.1    Shukin, R.2    Gillam, S.3
  • 9
    • 0025039837 scopus 로고
    • Processing and intracellular transport of rubella virus structural proteins in COS cells
    • Hobman, T.C., Lundström, M.L. and Gillam, S. (1990) Processing and intracellular transport of rubella virus structural proteins in COS cells. Virology 178, 122-133.
    • (1990) Virology , vol.178 , pp. 122-133
    • Hobman, T.C.1    Lundström, M.L.2    Gillam, S.3
  • 10
    • 0027469910 scopus 로고
    • The rubella virus E2 and E1 spike glycoproteins are targeted to the Golgi complex
    • Hobman, T.C., Woodward, L. and Farquhar, M.G. (1993) The rubella virus E2 and E1 spike glycoproteins are targeted to the Golgi complex. J. Cell. Biol. 121, 269-281.
    • (1993) J. Cell. Biol. , vol.121 , pp. 269-281
    • Hobman, T.C.1    Woodward, L.2    Farquhar, M.G.3
  • 11
    • 0028065160 scopus 로고
    • Assembly of rubella virus structural proteins into virus-like particles in transfected cells
    • Hobman, T.C., Lundström, M.L., Mauracher, C.A., Woodward, L., Gillam, S. and Farquhar, M.G. (1994a) Assembly of rubella virus structural proteins into virus-like particles in transfected cells. Virology 202, 574-585.
    • (1994) Virology , vol.202 , pp. 574-585
    • Hobman, T.C.1    Lundström, M.L.2    Mauracher, C.A.3    Woodward, L.4    Gillam, S.5    Farquhar, M.G.6
  • 12
    • 0028266760 scopus 로고
    • Expression of soluble forms of rubella virus glycoproteins in mammalian cells
    • Hobman, T.C., Seto, N.O.L. and Gillam, S. (1994b) Expression of soluble forms of rubella virus glycoproteins in mammalian cells. Virus Res. 31, 277-289.
    • (1994) Virus Res. , vol.31 , pp. 277-289
    • Hobman, T.C.1    Seto, N.O.L.2    Gillam, S.3
  • 13
    • 0029064295 scopus 로고
    • Targeting of a heterodimeric membrane protein complex to the Golgi: Rubella virus E2 glycoprotein contains a transmembrane Golgi retension signal
    • Hobman, T.C., Woodward, L. and Farquhar, M.G. (1995) Targeting of a heterodimeric membrane protein complex to the Golgi: rubella virus E2 glycoprotein contains a transmembrane Golgi retension signal. Mol. Biol. Cell 6, 7-20.
    • (1995) Mol. Biol. Cell , vol.6 , pp. 7-20
    • Hobman, T.C.1    Woodward, L.2    Farquhar, M.G.3
  • 14
    • 0022525736 scopus 로고
    • Use of surface aerator to improve oxygen transfer in cell culture
    • Hu, W.S., Meier, J. and Wang, D.I.C. (1986) Use of surface aerator to improve oxygen transfer in cell culture. Biotechnol. Bioeng. 28, 122-125.
    • (1986) Biotechnol. Bioeng. , vol.28 , pp. 122-125
    • Hu, W.S.1    Meier, J.2    Wang, D.I.C.3
  • 15
    • 0021170332 scopus 로고
    • Three genes code for rubella virus structural proteins E1. E2a. E2b and C
    • Kalkkinen, N., Oker-Blom, C. and Pettersson, R.F. (1984) Three genes code for rubella virus structural proteins E1. E2a. E2b and C. J. Gen. Virol. 65, 1549-1557.
    • (1984) J. Gen. Virol. , vol.65 , pp. 1549-1557
    • Kalkkinen, N.1    Oker-Blom, C.2    Pettersson, R.F.3
  • 16
    • 0028811610 scopus 로고
    • Purification of recombinant Glur-D glutamate receptor produced in Sf21 insect cells
    • Kuusinen, A., Arvola, M., Oker-Blom, C. and Keinänen, K. (1995) Purification of recombinant Glur-D glutamate receptor produced in Sf21 insect cells. Eur. J. Biochem. 233, 720-726.
    • (1995) Eur. J. Biochem. , vol.233 , pp. 720-726
    • Kuusinen, A.1    Arvola, M.2    Oker-Blom, C.3    Keinänen, K.4
  • 17
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during assembly of the head of bacteriophage T4
    • Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 18
    • 0002704281 scopus 로고
    • Cloning and expression of heterologous genes in insect cells with baculovirus vectors
    • A. Procop, R. K. Bajpai, and C. Ho (eds.) McGraw-Hill, New York
    • Luckow, V.A. (1991) Cloning and expression of heterologous genes in insect cells with baculovirus vectors. In: A. Procop, R. K. Bajpai, and C. Ho (eds.). Recombinant DNA Technology and Applications. McGraw-Hill, New York, pp. 97-152.
    • (1991) Recombinant DNA Technology and Applications , pp. 97-152
    • Luckow, V.A.1
  • 19
    • 0024152865 scopus 로고
    • Baculoviruses as gene expression vectors
    • Miller, L.K. (1988) Baculoviruses as gene expression vectors. Annu. Rev. Microbiol. 42, 177-179.
    • (1988) Annu. Rev. Microbiol. , vol.42 , pp. 177-179
    • Miller, L.K.1
  • 21
    • 0020577797 scopus 로고
    • Rubella virus contains one capsid protein and three envelope glycoproteins, E1, E2a and E2b
    • Oker-Blom, C., Kalkkinen, N., Kääriäinen, L. and Pettersson, R.F. (1983) Rubella virus contains one capsid protein and three envelope glycoproteins, E1, E2a and E2b. J. Virol. 46, 964-973.
    • (1983) J. Virol. , vol.46 , pp. 964-973
    • Oker-Blom, C.1    Kalkkinen, N.2    Kääriäinen, L.3    Pettersson, R.F.4
  • 22
    • 0021360118 scopus 로고
    • Rubella virus 40S genome RNA specifies a 24S mRNA that codes for a precursor to structural proteins
    • Oker-Blom, C., Ulmanen, I., Kääriäinen, L. and Pettersson, R.F. (1984) Rubella virus 40S genome RNA specifies a 24S mRNA that codes for a precursor to structural proteins. J. Virol. 49, 403-408.
    • (1984) J. Virol. , vol.49 , pp. 403-408
    • Oker-Blom, C.1    Ulmanen, I.2    Kääriäinen, L.3    Pettersson, R.F.4
  • 23
    • 0024447392 scopus 로고
    • Baculovirus polyhedrin promoter-directed expression of rubella virus envelope glycoproteins, E1 and E2, in Spodoptera frugiperda cells
    • Oker-Blom, C., Pcttersson, R.F. and Summers, M.D. (1989) Baculovirus polyhedrin promoter-directed expression of rubella virus envelope glycoproteins, E1 and E2, in Spodoptera frugiperda cells. Virology 172, 82-91.
    • (1989) Virology , vol.172 , pp. 82-91
    • Oker-Blom, C.1    Pcttersson, R.F.2    Summers, M.D.3
  • 24
    • 0025612120 scopus 로고
    • Translocation and cleavage of rubella virus envelope glycoproteins: Identification and role of the E2 signal sequence
    • Oker-Blom, C., Jarvis, D.L. and Summers, M.D. (1990) Translocation and cleavage of rubella virus envelope glycoproteins: identification and role of the E2 signal sequence. J. Gen. Virol. 71, 3047-3053.
    • (1990) J. Gen. Virol. , vol.71 , pp. 3047-3053
    • Oker-Blom, C.1    Jarvis, D.L.2    Summers, M.D.3
  • 25
    • 0028839976 scopus 로고
    • Synthesis and processing of the rubella virus p110 polyprotein precursor in baculovirus-infected Spodoptera frugiperda cells
    • Oker-Blom C., Blomster, M., Österblad, M., Schmidt, M., Akerman, K. and Lindqvist, C. (1995) Synthesis and processing of the rubella virus p110 polyprotein precursor in baculovirus-infected Spodoptera frugiperda cells. Virus Res. 35, 71-79.
    • (1995) Virus Res. , vol.35 , pp. 71-79
    • Oker-Blom, C.1    Blomster, M.2    Österblad, M.3    Schmidt, M.4    Akerman, K.5    Lindqvist, C.6
  • 27
    • 0028226159 scopus 로고
    • Expression and characterization of of virus-like particles containing rubella virus structural proteins
    • Qiu, Z., Ou, D., Wu, H., Hobman, T.C. and Gillam, S. (1994a) Expression and characterization of of virus-like particles containing rubella virus structural proteins. J. Virol. 68, 4086-4091.
    • (1994) J. Virol. , vol.68 , pp. 4086-4091
    • Qiu, Z.1    Ou, D.2    Wu, H.3    Hobman, T.C.4    Gillam, S.5
  • 28
    • 0028207632 scopus 로고
    • Mutational analysis of the arginine residues in the E2-E1 junction region on the proteolytic processing of the polyprotein precursor of rubella virus
    • Qiu, Z., McDonald, H.L., Chen, J., Hobman, T.C. and Gillam, S. (1994b) Mutational analysis of the arginine residues in the E2-E1 junction region on the proteolytic processing of the polyprotein precursor of rubella virus. Virology 200, 821-825.
    • (1994) Virology , vol.200 , pp. 821-825
    • Qiu, Z.1    McDonald, H.L.2    Chen, J.3    Hobman, T.C.4    Gillam, S.5
  • 29
    • 0022372670 scopus 로고
    • Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia
    • Saiki, R.K., Scharf. S., Faloona, F., Mullis, K.B., Horn, G.T., Erlich, H.A. and Arnheim, N. (1985). Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1350-1354.
    • (1985) Science , vol.230 , pp. 1350-1354
    • Saiki, R.K.1    Scharf, S.2    Faloona, F.3    Mullis, K.B.4    Horn, G.T.5    Erlich, H.A.6    Arnheim, N.7
  • 31
    • 0029880408 scopus 로고    scopus 로고
    • Detection of rubella virus specific IgM antibodies using a baculovirus-expressed E1 protein
    • in press
    • Schmidt, M., Lindqvist, C., Salmi, A. and Oker-Blom, C. (1996) Detection of rubella virus specific IgM antibodies using a baculovirus-expressed E1 protein. Clin. Diagn. Lab. Immunol. (in press).
    • (1996) Clin. Diagn. Lab. Immunol.
    • Schmidt, M.1    Lindqvist, C.2    Salmi, A.3    Oker-Blom, C.4
  • 33
    • 0028114828 scopus 로고
    • Expression and characterization of soluble rubella virus E1 envelope protein
    • Seto, N.O.L. and Gillam, S. (1994) Expression and characterization of soluble rubella virus E1 envelope protein. J. Med. Virol. 44, 192-199.
    • (1994) J. Med. Virol. , vol.44 , pp. 192-199
    • Seto, N.O.L.1    Gillam, S.2
  • 34
    • 0028801451 scopus 로고
    • Expresion and characterization of secreted forms of rubella virus E2 glycoprotein in insect cells
    • Seto, N.O.L., Dawe, O. and Gillam, S. (1995) Expresion and characterization of secreted forms of rubella virus E2 glycoprotein in insect cells. Virology 206, 736-741.
    • (1995) Virology , vol.206 , pp. 736-741
    • Seto, N.O.L.1    Dawe, O.2    Gillam, S.3
  • 35
    • 0028606112 scopus 로고
    • Identification of calreticulm as a rubella virus RNA binding protein
    • Singh, N.K., Atreya, C.D. and Nakhasi, H.L. (1994) Identification of calreticulm as a rubella virus RNA binding protein. Proc. Natl. Acad. Sci. USA 91, 12770-12774.
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 12770-12774
    • Singh, N.K.1    Atreya, C.D.2    Nakhasi, H.L.3
  • 38
    • 0026100208 scopus 로고
    • Enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide
    • Tessier, D.C., Thomas, D.Y., Khouri, H.H., Laliberté, F. and Vernet, T. (1991) Enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide. Gene 98, 177-183.
    • (1991) Gene , vol.98 , pp. 177-183
    • Tessier, D.C.1    Thomas, D.Y.2    Khouri, H.H.3    Laliberté, F.4    Vernet, T.5
  • 39
    • 0016757289 scopus 로고
    • Tunicamycin inhibition of polyisoprenol N-acetylglucosaminyl pyrophosphate formation in calf liver microsomes
    • Tkacz, J.S. and Lampen, O. (1975) Tunicamycin inhibition of polyisoprenol N-acetylglucosaminyl pyrophosphate formation in calf liver microsomes. Biochem. Biophys. Res. Commun. 65, 248-257.
    • (1975) Biochem. Biophys. Res. Commun. , vol.65 , pp. 248-257
    • Tkacz, J.S.1    Lampen, O.2
  • 41
    • 0009482260 scopus 로고
    • Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
    • Towbin, H., Stachelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
    • (1979) Proc. Natl. Acad. Sci. USA , vol.76 , pp. 4350-4354
    • Towbin, H.1    Stachelin, T.2    Gordon, J.3
  • 42
    • 0020626190 scopus 로고
    • Immunochemical identification of rubella virus hemagglutinin
    • Waxham, M.N. and Wolinski, J.S. (1983) Immunochemical identification of rubella virus hemagglutinin. Virology 126, 194-203.
    • (1983) Virology , vol.126 , pp. 194-203
    • Waxham, M.N.1    Wolinski, J.S.2
  • 44
    • 0029199998 scopus 로고
    • Insect cell-culture techniques in serum-containing media
    • C.D. Richardson (Ed.). Baculovirus Expression Protocols. Humana Press. Totowa. NJ
    • Weiss, S.A., Whitford, W.G., Gorfien, S.F. and Godwin, G.P. (1995a) Insect cell-culture techniques in serum-containing media. In: C.D. Richardson (Ed.). Methods in Molecular Biology. Vol. 39. Baculovirus Expression Protocols. Humana Press. Totowa. NJ. pp. 65-78.
    • (1995) Methods in Molecular Biology , vol.39 , pp. 65-78
    • Weiss, S.A.1    Whitford, W.G.2    Gorfien, S.F.3    Godwin, G.P.4
  • 45
    • 0029188122 scopus 로고
    • Insect cell-culture in serum-free media
    • C.D. Richardson (Ed.) Baculovirus Expression Protocols. Humana Press. Totowa. NJ
    • Weiss, S.A., Godwin, G.P., Gorfien, S.F. and Whitford, W.G. (1995b) Insect cell-culture in serum-free media. In: C.D. Richardson (Ed.). Methods in Molecular Biology, Vol. 39, Baculovirus Expression Protocols. Humana Press. Totowa. NJ. pp. 79-95.
    • (1995) Methods in Molecular Biology , vol.39 , pp. 79-95
    • Weiss, S.A.1    Godwin, G.P.2    Gorfien, S.F.3    Whitford, W.G.4
  • 46
    • 0002524182 scopus 로고
    • Rubella
    • B.N. Fields, D.M. Knipe et al. (Eds.), Raven Press, New York
    • Wolinsky, J.S. (1990). Rubella. In: B.N. Fields, D.M. Knipe et al. (Eds.), Virology, Second Edition. Raven Press, New York. pp. 815-837.
    • (1990) Virology, Second Edition , pp. 815-837
    • Wolinsky, J.S.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.