A Transformed Human Tracheal Gland Cell Line, MM-39, that Retains Serous Secretory Functions

American Journal of Respiratory Cell and Molecular Biology

Volumn 15, Issue 4, 1996, Pages 520-528

  Merten, Marc D ^a   Kammouni, Wafa ^b   Renaud, Wanda ^b   Birg, Françoise ^b   Mattéi, Marie Geneviève ^b   Figarella, Catherine ^b  

^a AIX MARSEILLE UNIVERSITY   (France)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BRONCHUS; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40; SV40 VIRUS;

MUCIN;

ARTICLE; CELL LINE; CYTOLOGY; HUMAN; METABOLISM; MUCOSA; SECRETION; SIMIAN VIRUS 40; TRACHEA; VIRUS CELL TRANSFORMATION;

CELL LINE, TRANSFORMED; CELL TRANSFORMATION, VIRAL; HUMANS; MUCINS; MUCOUS MEMBRANE; SIMIAN VIRUS 40; TRACHEA;

EID: 0030266920     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.15.4.8879186     Document Type: Article

References (31)

1. Reid, L. 1960. Measurement of the bronchial mucus layer: a diagnostic yardstick in chronic bronchitis. Thorax 15:132-141.
2. Engelhardt, J. F., J. R. Yankaskas, S. A. Ernst, Y. P. Yang, C. R. Marino, R. C. Boucher, J. A. Cohn, and J. M. Wilson. 1992. Submucosal glands are the predominant site of CFTR expression in human bronchus. Nature Genetics 2:240-246.
3. Jacquot, J., E. Puchelle, J. Hinnrasky, C. Fuchey, C. Bettinger, C. Spilmont, N. Bonnet, A. Dieterle, D. Dreyer, A. Pavirani, and W. Dalemans. 1993. Localization of the cystic fibrosis transmembrane conductance regulator in airway secretory glands. Eur. Respir. J. 6:169-176.
4. Riordan, J. R., J. M. Rommens, B. S. Kerem, N. Alon, R. Rozmahel, Z. Grzelczak, J. Zielenski, S. Lok, N. Plavsic, J. L. Chou, M. L. Drumm, M. C. Iannuzzi, F. S. Collins, and L. C. Tsui. 1989. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245:1066-1073.
5. Kerem, B. S., J. M. Rommens, J. A. Buchanan, D. Markiewicz, T. K. Cox, A. Chakravarti, M. Buchwald, and L. C. Tsui. 1989. Identification of the cystic fibrosis gene: genetic analysis. Science 245:1073-1080.
6. Tournier, J. M., M. D. Merten, Y. Meckler, J. Hinnrasky, C. Fuchey, and E. Puchelle. 1990. Culture and characterization of human tracheal gland cells. Am. Rev. Respir. Dis. 141:1280-1288.
7. Merten, M., J. M. Tournier, Y. Meckler, and C. Figarella. 1992. Secretory proteins and glycoconjugates synthesized by human tracheal gland cells in culture. Am. J. Respir. Cell Mol. Biol. 7:598-605.
8. Merten, M., J. M. Tournier, Y. Meckler, and C. Figarella. 1993. Epinephrine promotes growth and differentiation of human tracheal gland cells in culture. Am. J. Respir. Cell Mol. Biol. 9:172-178.
9. Merten, M., J. P. Breittmayer, C. Figarella, and C. Frelin. 1993. ATP and UTP increase the secretion of the bronchial inhibitor by human tracheal gland cells in culture. Am. J. Physiol. 265:L479-L484.
10. Becq, F., M. D. Merten, M. A. Voelckel, M. Gola, and C. Figarella. 1993. Characterization of cAMP-dependent CFTR-chloride channels in human tracheal gland cells. FEBS Lett. 321:73-78.
11. Renaud, W., M. Merten, and C. Figarella. 1994. Increased coexpression of CFTR and S100 calcium binding proteins MRP8 and MRP14 in cystic fibrosis human tracheal gland cells. Biochem. Biophys. Res. Commun. 201, 3:1518-1525.
12. Merten, M., and C. Figarella. 1993. Constitutive hypersecretion and insensitivity to neurotransmitters by cystic fibrosis tracheal gland cells. Am. J. Physiol. 264:L93-L99.
13. Kramps, J., C. Franken, and J. Dijkman. 1981. Localisation of low molecular weight protease inhibitor in serous secretory cells of the respiratory tract. J. Histochem. Cytochem. 29:712-720.
14. Merten, M., W. Renaud, F. Birg, M. G. Mattei, and C. Figarella. 1994. Establishment and characterization of a cell line, MM39, transformed from tracheal gland serous cells and retaining their secretory characteristics. Pediatr. Pulm. (Suppl.):10:245.
15. Chen, T. R. 1977. In situ detection of Mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell. Res. 104:255-262.
16. Birg, F., R. Dulbecco, M. Fried, and R. Kamen. 1979. State and organization of polyoma virus DNA sequence in transformed rat cell lines. J. Virol. 29:633-648.
17. Tooze, J. 1981. DNA Tumor Viruses, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
18. Gluzman, Y., R. J. Frisque, and J. Sambrook. 1980. Origin-defective mutants of SV40. Cold Spring Harbor Symp. Quant. Biol. XLIV:293-300.
19. Jacquot, J., C. Spilmont, H. Burlet, C. Fuchet, A. C. Buisson, J. M. Tournier, D. Gaillard, and E. Puchelle. 1994. Glandular like morphogenesis and secretory activity of tracheal gland cells in a three-dimensional collagel gel matrix. J. Cell Physiol. 161:407-418.
20. Tournier, J. M., J. Jacquot, P. Sadoul, and J. G. Bieth. 1983. Non competitive enzyme immunoassay for the measurement of bronchial inhibitor in biological fluids. Anal. Biochem. 131:345-350.
21. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the heads of bacteriophage T4. Nature 227:680-685.
22. Leong, M. M. L., G. R. Fox, and J. S. Hayward. 1988. A photodetection device for luminal-based immunodot and western blotting assays. Anal. Biochem. 168:107-114.
23. Chirgwin, J. M., A. E. Przybyla, R. J. MacDonald, and W. J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 19:5294-5299.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. In Molecular Cloning, A Laboratory Manual. C. Nolan, editor. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Book 1, Chap. 7.
25. Fonknechten, N., J. Chelly, J. Lepercq, A. Kahn, J. C. Kaplan, A. Kitzis, and J. C. Chomel. 1992. CFTR illegitimate transcription in lymphoid cells: quantification and application to the investigation of pathological transcripts. Hum. Genet. 88:508-512.
26. Jefferson D. M., J. D. Valentich, F. C. Marini, S. A. Grubman, M. C. Iannuzzi, H. L. Dorkin, M. Li, K. W. Klinger, and M. J. Welsh. 1990. Expression of normal and cystic fibrosis phenotypes by continuous airway epithelial cell lines. Am. J. Physiol. 259:L496-L505.
27. Zeitlin, P. L., L. Lu, J. Rhim, G. Cutting, G. Stetten, K. A. Kieffer, R. Craig, and W. B. Guggino. 1991. A cystic fibrosis bronchial epithelial cell line: immortalization by adeno-12-SV40 infection. Am. J. Respir. Cell Mol. Biol. 4:313-319.
28. Yankaskas, J. R., J. E. Haizlip, M. Conrad, D. Koval, E. Lazarowski, A. M. Paradiso, C. A. Rinehart, Jr., B. Sardaki, R. Schlegel, and R. C. Boucher. 1993. Papilloma virus immortalized tracheal epithelial cells retain a well-differentiated phenotype. Am. J. Physiol. 264:C1219-C1230.
29. Cozens, A. L., M. J. Yezzi, L. Chin, E. M. Simon, W. E. Finkbeiner, L. A. Wagner, and D. C. Gruenert. 1992. Characterization of immortal cystic fibrosis tracheobronchial gland epithelial cells. Proc. Natl. Acad. Sci. USA 89:5171-5175.
30. Chopra, D., G. W. Taylor, P. A. Mathieu, B. Hukku, and J. Rhim. 1991. Immortalization of human tracheal gland epithelial cells by adenovirus 12-SV40 hybrid virus. In Vitro Cell. Dev. Biol. 27A:763-765.
31. Yoshimura, K., H. Nakamura, B. C. Trapnell, C. H. Chu, W. Dalemans, A. Pavirani, J. P. Lecocq, and R. G. Crystal. 1991. Expression of the cystic fibrosis transmembrane conductance regulator gene in cells of non-epithelial origin. Nucleic Acids Res. 19:5417-5423.