Affinity precipitation of Concanavalin A with p-Aminophenyl-α-D-glucopyranoside modified Eudragit S-100: II. Kinetic studies of the formation and the dissociation of the protein-macroligand complex

Bioseparation

Volumn 6, Issue 5, 1996, Pages 283-291

  Larsson, Eva Linné ^a   Galaev, Igor Yu ^a   Mattiasson, Bo ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Affinity precipitation; Concanavalin A; Dissociation of complexes; Dynamic laser light scattering; Eudragit S 100; Initial rate of complex formation

Indexed keywords

CHEMICAL MODIFICATION; COPOLYMERS; DISSOCIATION; LASER APPLICATIONS; LIGHT SCATTERING; PROTEINS; REACTION KINETICS;

AFFINITY PRECIPITATION; AMINOPHENYL GLUCOPYRANOSIDE; CONCANAVALIN A; DYNAMIC LASER LIGHT SCATTERING; EUDRAGIT;

PRECIPITATION (CHEMICAL);

EID: 0030265252     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Agrawal BBL and Goldstein IJ (1968) Protein-carbohydrate interaction. VII Physical and chemical studies on Concanavalin A, the Hemagglutinin of the Jack bean. Arch. Biochem. Biophys. 124: 218-229.
2. Borrebaeck C and Mattiasson B (1980) Lectin-carbohydrate interactions studied by a competitive enzyme inhibition assay. Anal. Biochem. 107: 446-450.
3. Bittiger H and Schnebli HP (eds) (1976) Concanavalin A as a tool., John Wiley & Sons Ltd., New York.
4. Brown W (ed) (1993) Dynamic light scattering - The method and some applications., Clarendon Press, Oxford.
5. Guoqiang D, Benhura MAN, Kaul R and Mattiasson B (1995) Affinity thermoprecipitation of yeast alcohol dehydrogenase through metal ion promoted binding with Eudragit Bound Cibacran blue 3GA. Biotechnology Progress 11 (2): 187-193.
6. Gupta MN and Mattiasson B (1994) Affinity precipitation. In: Street, G. (ed) Highly selective separations in biotechnology, (pp. 7-33) Blackie Academic and Professional, an imprint of Chapman & Hall, Glasgow.
7. Harding SE, Sattelle DB and Bloomfield VA (eds) (1992) Laser light scattering in biochemistry. Redwood Press Ltd., Cambridge, (The Royal Society of Chemistry cop.).
8. Honda S, Suzuki S and Kakehi K (1987) Separation of carbohydrates and lectins on carbohydrate-immobilized resins. J. Chromatogr. 396: 93-100.
9. Irwin JA and Tipton KF (1995) Affinity precipitation: a novel approach to protein purification. Essays Biochem. 29: 137-156.
10. Iyer RN and Goldstein U (1973) Quantitative studies on the interaction of Concanavalin A, the carbohydrate-binding protein of the Jack bean, with model carbohydrate-protein conjugates. Immunochemistry 10: 313-322.
11. Kamihira M, Kaul R and Mattiasson B (1992) Purification of recombinant protein A by aqueous two phase extraction integrated with affinity precipitation. Biotechnol. Bioeng. 40: 1381-1387.
12. Linné Larsson E, Galaev IY, Lindahl L & Mattiasson B (1996) Affinity precipitation of Concanavalin A with p-aminophenyl-α-D-glucopyranoside modified Eudragit S-100. I. Initial complex formation and build-up of the precipitate. Bioseparation 6: 273-282.
13. Linné Larsson E and Mattiasson B (1994) Isolation of Concanavalin A by affinity precipitation. Biotechnol. Techn. 8: 51-56.
14. Poretz RD and Goldstein IJ (1970) An examination of the topology of the saccharide binding sites of Concanavalin A and of the forces involved in complexation. Biochemistry 9 (14): 2890-2896.
15. Schmitz KS (1990) An introduction to dynamic light scattering by macromolecules., Academic Press, Boston.
16. Shu H-C, Guoqiang D, Kaul R and Mattiasson B (1994) Purification of the D-lactate dehydrogenase from Leuconostoc mesenteroides ssp. cremoris using a sequential precipitation procedure. J. Biotechnol. 34: 1-11.
17. So L, L and Goldstein IJ (1968) Protein-carbohydrate interaction. XX On the number of combining sites on Concanavalin A, the phytohemagglutinin of the Jack bean. Biochim. Biophys. Acta 165: 398-404.
18. Vretblad P (1976) Purification of lectins by biospecific affinity chromatography. Biochim. Biophys. Acta 434: 169-176.