Wild-type and mutant forms of recombinant horseradish peroxidase C expressed in Escherichia coli: Substrate specificity and stability under irradiation

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 61, Issue 1-2, 1996, Pages 13-23

  Mareeva, E A ^a   Orlova, M A ^a   Doseeva, V V ^a   Loginov, D B ^a   Galkin, A G ^a   Gazarian, I G ^a   Tishkov, V I ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

ABTS; Hydrogen peroxide; Inactivation; Iodide; Low dosage radiolysis; Rate constants; Site specific mutagenesis; Substrate binding sites; Substrate specificity

Indexed keywords

HORSERADISH PEROXIDASE; ISOENZYME; RECOMBINANT PROTEIN;

ARTICLE; BINDING SITE; CHEMICAL MODEL; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

BINDING SITES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HORSERADISH PEROXIDASE; ISOENZYMES; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 0030257046     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02785684     Document Type: Article

References (22)

1. Finzel, B. C., Poulos, T. L., and Kraut, J. (1984), J. Biol. Chem. 259, 13,027-13,036.
2. Sandaramoorthy, M., Kishi, K., Gold, M. H., and Poulos, T. L. (1994), J. Mol. Biol. 238, 845-848.
3. Edwards, S. L., Raag, R., Wariishi, H., Gold, M. H., and Poulos, T. L. (1993), Proc. Natl. Acad. Sci. USA 90, 750-754.
4. Petersen, J. W. F., Kadziola, A., and Larsen, S. (1993), in Plant Peroxidases: Biochemistry and Physiology, Welinder, K. G., ed., Geneva University, Geneve, pp. 15-20.
5. Welinder, K. G. (1992), Curr. Opinion Struct. Biol. 2, 388-393.
6. Smith, A. T., Sanders, S. A., Sampson, C., Bray, R. C., Burke, J. F., and Thorneley, R. N. F. (1993), in Plant Peroxidases: Biochemistry and Physiology, Welinder, K. G., ed., Geneva University, Geneve, pp. 159-168.
7. Smith, A. T., Santama, N., Dacey, S., Edwards, M., Bray, R. C., Thorneley, R. N., and Burke, J. F. (1990), J. Biol. Chem. 265, 13,335-13,343.
8. Egorov, A. M., Gazaryan, I. G., Kim, B. B., Doseeva, V. V., Kapeliuch, J. L., Veryovkin, A. N., and Fechina, V. A. (1994), Ann. NY Acad. Sci 721, 73-82.
9. Gazaryan, I. G., Doseeva, V. V., Galkin, A. G., and Tishkov, V. I. (1994), FEBS Lett. 354, 248-251.
10. Childs, R. E. and Bardsley, W. G. (1975), Biochem. J. 145, 93-103.
11. Gallati, H. (1977), J. Clin. Chem. Clin. Biochem. 23, 41-44.
12. Hosoya, T. and Morrison, M. (1967), J. Biol. Chem. 242, 2828-2836.
13. Ugarova, N. N., Rozhkova, G. D., and Berezin, I. V. (1979), Biochim. Biophys. Acta 570, 31-38.
14. Portsman, T. and Portsman, B. (1985), J. Clin. Chem. Clin. Biochem. 23, 41-44.
15. Hasinoff, F. and Dunford, H. B. (1970), Biochemistry 9, 4930-4939.
16. Banerjee, R. K., De, S. K., Bose, A. O., and Datta, A. G. (1986), J. Biol. Chem. 261, 10,502-10,507.
17. Furhop, J. H. and Smith, K. M. (1975), in Porphyrins and Metalloproteins, Smith, K. M., ed., Elsevier, Amsterdam, pp. 757-803.
18. Orlova, M. A., Mareeva, E. A., Doseeva, V. V., and Gazarian, I. G. (1994), Izvestiya RAN, Ser. Khim. (Russ. Chem. Bull.) 12, 2230-2233.
19. Gazarian, I. G., Doseeva, V. V., Mareeva, E. A., and Orlova, M. A. (1994), Izvestiya RAN, Ser. Chem. (Russ. Chem. Bull.) 12, 2234-2237.
20. Smith, A. T., Sanders, S. A., Thorneley R. N. F., Burke, J. F., and Bray, R. C. (1992), Eur. J. Biol. Chem. 207, 507-519.
21. Hartmann, C. and Ortiz de Montellano, P. R. (1992), Arch. Biochem. Biophys. 297, 61-72.
22. Pappa, H. S. and Cass, A. E. G. (1993), Eur. J. Biochem. 212, 227-238.