Evaluation of ploidy of mature canine megakaryocytes, using Feulgen staining and microspectrophotometry

American Journal of Veterinary Research

Volumn 57, Issue 10, 1996, Pages 1434-1437

  Boudreaux, Mary K ^a   Ebbe, Shirley ^b  

^a AUBURN UNIVERSITY   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COLORING AGENT; DNA; FUCHSINE;

ANALYSIS OF VARIANCE; ANIMAL; ARTICLE; BONE MARROW; CYTOLOGY; DOG; FEULGEN STAINING; FLOW CYTOMETRY; HUMAN; MEGAKARYOCYTE; MICROSPECTROPHOTOMETRY; PLOIDY; THROMBOCYTE COUNT;

ANALYSIS OF VARIANCE; ANIMALS; BONE MARROW; COLORING AGENTS; DNA; DOGS; FLOW CYTOMETRY; HUMANS; MEGAKARYOCYTES; MICROSPECTROPHOTOMETRY; PLATELET COUNT; PLOIDIES; ROSANILINE DYES;

EID: 0030256835     PISSN: 00029645     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Mazur EM. Megakaryocytopoiesis and platelet production: a review. Exp Hematol 1987;15:340-350.
2. Fand SB. Environmental conditions for optimal Feulgen hydrolysis. In: Weid GL, Bahr GF, eds. Introduction of quantitative cytochemistry-II. New York: Academic Press Inc, 1970;209-221.
3. Ornstein L. The distributional error in microspectrophotometry. Lab Invest 1952;1:250-262.
4. Patau K. Absorption microphotometry of irregular-shaped objects. Chromosoma 1952;5:341-362.
5. Alpen EL. A new instrument for precision cytophotometry. J Sci Instru 1966;43:443-448.
6. d mice. Blood Cells 1986;12:217-232.
7. v mice is accompanied by an increase in size within ploidy groups and acceleration of maturation. Blood 1989;74:94-98.
8. Odell TT Jr, Jackson CW, Gosslee DG. Maturation of rat megakaryocytes studied by microspectrophotometric measurement of DNA. Proc Soc Exp Biol Med 1965;119:1194-1199.
9. Odell TT Jr, Jackson CW. Polyploidy and maturation of rat megakaryocytes. Blood 1968;32:102-110.
10. Penington DG, Olsen TE. Megakaryocytes in states of altered platelet production: cell numbers, size and DNA content. Br J Haematol 1970;18:447-463.
11. Tanum G, Engeset A. Low ploidy megakaryocytes in steady-state rat bone marrow. Blood 1983;62:87-91.
12. Queisser U, Queisser W, Spiertz B. Polyploidization of megakaryocytes in normal humans, in patients with idiopathic thrombocytopenia and with pernicious anaemia. Br J Haematol 1971;20:489-501.
13. Paulus JM. DNA metabolism and development of organelles in guinea-pig megakaryocytes: a combined ultrastructural, autoradiographic and cytophotometric study. Blood 1970;35:298-311.
14. Garcia AM. Studies on DNA in leucocytes and related cells of mammals. IV. The Feulgen-DNA content of peripheral leucocytes, megakaryocytes and other bone marrow cell types of the rabbit. Acta Histochem 1964;17:246-258.
15. Garcia AM. Feulgen-DNA values in megakaryocytes. J Cell Biol 1964;20:342-345.
16. Ebbe S, Dalal K, Forte T, et al. Microcytic thrombocytosis, small megakaryocytes, platelet lipids and hyperreactivity to collagen, lymphocytopenia, eosinophilia, and low blood volume in genetically hyperlipidemic rabbits. Exp Hematol 1992;20:486-493.
17. Ebbe S, Maurer H. Size and ploidy of rabbit megakaryocytes in dietary hypercholesterolaemia. Comp Haematol Int 1992;2:208-212.
18. Worthington RE, Nakeff A, Micko S. Flow cytometric analysis of megakaryocyte differentiation. Cytometry 1984;5:501-508.
19. Jackson CW, Brown LK, Somerville BC, et al. Two-color flow cytometric measurement of DNA distributions of rat megakaryocytes in unfixed, unfractionated marrow cell suspensions. Blood 1984;63:768-778.
20. Burstein SA, Friese P, Downs T, et al. Canine megakaryocytopoiesis: analysis utilizing a monoclonal antibody to a 140-kD dog platelet protein. Exp Hematol 1991;19:47-52.
21. Burstein SA, Downs T, Friese P, et al. Thrombocytopoiesis in normal and sublethally irradiated dogs: response to human interleukin-6. Blood 1992;80:420-428.
22. Ebbe S, Stohlman F Jr. Megakaryocytopoiesis in the rat. Blood 1965;26:20-35.
23. Zar JH. Biostatistical analysis. 2nd ed. Englewood Cliffs, NJ: Prentice-Hall Inc, 1984;199-201.
24. Corash L. The relationship between megakaryocyte ploidy and platelet volume. Blood Cells 1989;15:81-107.
25. Tomer A, Friese P, Conklin R, et al. Flow cytometric analysis of megakaryocytes from patients with abnormal platelet counts. Blood 1989;74:594-601.
26. Nakeff A, Valeriote F, Gray JW, et al. Application of flow cytometry and cell sorting to megakaryocytopoiesis. Blood 1979;53: 732-745.
27. Kuter DJ, Rosenberg RD. Regulation of megakaryocyte ploidy in vivo in the rat. Blood 1990;75:74-81.
28. Corash L, Chen HY, Levin J, et al. Regulation of thrombopoiesis: effects of the degree of thrombocytopenia on megakaryocyte ploidy and platelet volume. Blood 1987;70:177-185.
29. Long MW, Williams N, Ebbe S. Immature megakaryocytes in the mouse: physical characteristics, cell cycle status, and in vitro responsiveness to thrombopoietic stimulatory factor. Blood 1982;59: 569-575.