Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277

Journal of Periodontal Research

Volumn 31, Issue 7, 1996, Pages 477-482

  Johansson, Anders ^a   Bergenholtz, Axel ^a   Holm, Stig E ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

Cytotoxicity; Fusobacterium nucleatum; Gingival fibroblasts, Actinobacillus actinomycetemcomitans; Neutral red uptake; Porphyromonas gingivalis

Indexed keywords

ANTISERUM; CYTOTOXIN; NEUTRAL RED;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ARTICLE; CELL CULTURE; CELL DEATH; CULTURE MEDIUM; CYTOLOGY; DRUG EFFECT; EPITHELIUM; EPITHELIUM CELL; FIBROBLAST; FUSOBACTERIUM NUCLEATUM; GINGIVA; HELA CELL; HUMAN; METABOLISM; MICROBIOLOGY; PATHOGENICITY; PORPHYROMONAS GINGIVALIS;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; CELL DEATH; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; CYTOTOXINS; EPITHELIAL CELLS; EPITHELIUM; FIBROBLASTS; FUSOBACTERIUM NUCLEATUM; GINGIVA; HELA CELLS; HUMANS; IMMUNE SERA; NEUTRAL RED; PORPHYROMONAS GINGIVALIS;

EID: 0030254579     PISSN: 00223484     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0765.1996.tb01412.x     Document Type: Article

References (32)

1. Listgarten MA. Nature of periodontal disease: pathogenic mechanisms. J Periodont Res 1987; 22: 172-178.
2. Socransky SS, Haffajee AD. The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol 1992; 63: 322-331.
3. Moore WEC. Microbiology of periodontal disease. J Periodont Res 1987; 22: 335-341.
4. Slots J, Feik D, Rams TE. Actinobacillus actinomycetemcomitans and Bacteroides intermedius in human periodontitis: age relationship and mutual association. J Clin Periodontol 1990; 17: 659-662.
5. Socransky SS, Haffajee AD, Dzink JL, Hillman JD. Associations between microbial species in subgingival plaque samples. Oral Microbiol Immunol 1988; 3: 1-7.
6. Crossner CG, Carlsson J, Sjödin B, Tärnvik A, Unell L, Venge P, Wranne L. Periodontitis in the primary dentition associated with Actinobacillus actinomycetemcomitans infection and leukocyte dysfunction. J Clin Periodontol 1990; 17: 264-267.
7. Evans RI, Mikulesky M, Seymour GJ. Effect of initial treatment of chronic inflammatory periodontal disease in adults on spontaneous peripheral blood lymphocyte proliferation. J Clin Periodontol 1989; 16: 271-277.
8. Dahlèn G. Role of suspected periodontal periodontopathogens in microbiological monitoring of periodontitis. Adv Dent Res 1993; 7: 163-174.
9. Michalowicz BS. Genetic and inheritance in periodontal disease. Curr Opin Periodontol 1993; 11-17.
10. Offenbacher S, Collins JG, Arnold RR. New clinical diagnostic strategies based on pathogenesis of disease. J Periodont Res 1993; 28: 523-535.
11. Stevens RH, Hammond BF. The comparative cytotoxicity of periodontal bacteria. J Periodontol 1988; 59: 741-749.
12. Shenker BJ, Kushner ME, Tsai C-C. Inhibition of fibroblast proliferation by Actinobacillus actinomycetemcomitans. Infect Immun 1982; 38: 986-992.
13. Larjava H, Inhibition of gingival fibroblast growth by Bacteroides gingivalis. Infect Immun 1987; 55: 201-205.
14. Bartold PM, Gully NJ, Zlim PS, Rogers AH. Identification of components in Fusobacterium nucleatum chemostatculture supernatants that are potent inhibitors of human gingival fibroblast proliferation. J Periodont Res 1991; 26: 314-321.
15. Johansson A, Bergenholtz A, Holm SE. Interaction amongst some common oral bacteria in vitro. Bacterial toxicity on oral tissue cells cells. J Periodont Res 1994; 29: 318-323.
16. Spitznagel JR, Kraig E, Kolodrubetz D. Regulation of leukotoxin and leukotoxic strains of Actinobacillus actinomycetemcomitans. Infect Immun 1991; 59: 1394-1401.
17. McArthur WP, Tsai C-C, Baehni PC, Genco RJ, Taichman NS. Leukotoxic effects of Actinobacillus actinomycetemcomitans. Modulating by serum components, J Periodont Res 1981; 16: 159-170.
18. Gunsolley JC, Ranney RR, Zambon JJ, Burmeister JA, Schenkein HA. Actinobacillus actinomycetemcomitans in families afflicted with periodontitis. J Periodontol 1990; 61: 643-648.
19. Van Steenbergen TJM, De Graaff J. Proteolytic effect of black pigmented Bacteroides strains, FEMS Microbiol Lett 1986; 33: 219-222.
20. Johansson A, Bergenholtz A, Holm SE. Interaction amongst common oral bacteria in vitro. Growth interference. APMIS 1994; 102: 810-816.
21. Carlsson J, Frölander F, Sundquist G. Oxygen tolerance of anaerobic bacteria isolated from necrotic dental pulps. Acta Odont Scand 1976; 35: 139-145.
22. Hunt DE, Jones JV, Dowell VR Jr. A selective medium for isolation of Bacteroides gingivalis. J Clin Microbiol 1985; 23: 441-445.
23. Holm SE, Falsen E. Antigen-free medium for cultivation of beta-hemolytic Streptococci. Acta Path Microbiol Scand 1967; 69: 264-276.
24. Borenfreund E, Puerener JA. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). J Tiss Culture Meth 1984; 9: 7-9.
25. Borenfreund E, Puerener J A. Toxicity determined in vitro by morphological and alterations and neutral red absorption. Toxicol Lett 1985; 24: 119-124.
26. Ebersole JL, Steffen MJ. Human antibody responses to outer envelope antigens of Porphyromonas gingivalis serotypes. J Periodont Res 1995; 30: 1-14.
27. Beck JD, Koch GG, Zambon JJ, Genco RJ, Tudor GE. Evaluation of oral bacteria as a risk indicators for periodontitis in older adults. J Periodontol 1992; 63: 93-99.
28. Barthold PM, Haynes DR. Interleukin-6 production by human gingival fibroblasts. J Periodont Res 1991; 26: 339-345.
29. Ronai P. The elution of Cr-51 leukocytes - a new theory. Blood 1969; 33: 408-413.
30. Borenfreund E, Babich H, Martin-Alguacil N. Comparison of two in vitro cytotoxicity assays - the neutral red (NR9 and terazolium MTT tests. Toxic in Vitro 1988; 2: 1-6.
31. Saglie FR, Marfany A, Camargo P. Intragingival occurrence of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in active destructive periodontal lesions. J Periodontol 1988; 59: 259-265.
32. Sreenivasan PK, Meyer DH, Fives-Taylor PM. Requirements for invasion of epithelial cells by Actinobacillus actinomycetemcomitans. Infect Immun 1993; 61: 1239-1245.