Recombinant human phenylethanolamine N-methyltransferase: Overproduction in Escherichia coli, purification, and characterization

Protein Expression and Purification

Volumn 8, Issue 2, 1996, Pages 160-166

  Caine, Joanne M ^a   Macreadie, Ian G ^b   Grunewald, Gary L ^c   McLeish, Michael J ^a  

^a MONASH UNIVERSITY   (Australia)

^b CSIRO   (Australia)

^c UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

EID: 0030250712     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0088     Document Type: Article

References (26)

1. 1. Kirshner, N., and Goodall, M. (1957) The formation of adrenaline form noradrenaline. Biochim. Biophys. Acta. 24, 658-659.
2. 2. Axelrod, J. (1962) Purification and properties of phenylethanolamine N-methyltransferase J. Biol. Chem. 237, 1657-1660.
3. 3. Hokfelt, T., Fuxe, K., Goldstein, M., and Johannson, O. (1973) Evidence for adrenaline neurons in the rat brain. Acta. Physiol. Scand. 89, 286-289.
4. 4. Hokfelt, T., Fuxe, K., Goldstein, M., and Johannson, O. (1974) Immunohistochemical evidence for the existence of adrenaline neurons in the rat brain. Brain Res. 66, 235-251.
5. 5. Pickel, V. M., Chan, J., Park, D. H., Joh, T. H., and Milner, T. (1986) Ultrastructural localization of phenylethanolamine N-methyltransferase in sensory and motor nuclei of the vagus nerve. J. Neurosci. Res. 15, 439-455.
6. 6. Bohn, M. C. (1986) Expression and development of phenylethanolamine N-methyltransferase (PNMT): Role of glucocorticoids. Neurol. Neurobiol. (NY) 16, 245-271.
7. 7. Connet, R. J., and Kirshner, N. (1970) Purification and properties of bovine phenylethanolamine N-methyltransferase. J. Biol. Chem. 245, 329-334.
8. 8. Lee, H., Schulz, A. R., and Fuller, R. W. (1978) Isolation and purification of rabbit adrenal norepinephrine N-methyltransferase isozymes. Arch. Biochem. Biophys. 185, 222-227.
9. 9. Pohorecky, L. A., and Baliga, B. S. (1973) Purification and properties of rat adrenal phenylethanolamine N-methyltransferase. Arch. Biochem. Biophys. 156, 703-711.
10. 10. Baetge, E. E., Suh, Y. H., and Joh, T. H. (1986) Complete nucleotide and deduced amino acid sequence of bovine phenylethanolamine N-methyltransferase: Partial amino acid homology with rat tyrosine hydroxylase. Proc. Natl. Acad Aci USA 83, 5454-5458.
11. 11. Batter, D. K., D'Mello, S. R., Turzai, L. M., Hughes III, H. B., Gioio, A. E., and Kaplan, B. B. (1988) The complete nucleotide sequence and structure of the gene encoding bovine phenylethanolamine N-methyltransferase. J. Neurosci. Res. 19, 367-376.
12. 12. Kaneda, N., Ichinose, H., Kobayashi, K., Oka, K., Kishi, F., Nakazawa, A., Kurosawa, Y., Fujita, K., and Nagatsu, T. (1988) Molecular cloning of cDNA and chromosomal assignment of the gene for human phenylethanolamine N-methyltransferase, the enzyme for epinephrine biosynthesis. J. Biol. Chem. 263, 7672-7677.
13. 13. Weisberg, E. P., Baruchin, A., Stachowiak, M. K., Stricker, E. M., Zigmond, M. J., and Kaplan, B. B. (1989) Isolation of a rat adrenal cDNA clone encoding phenylethanolamine N-methyltransferase and cold induced alterations in adrenal PNMT mRNA and protein. Mol. Brain. Res. 6, 159-166.
14. 14. Suh, Y.-H., Chun, Y.-S., Lee, I. S., Kim, S.-S., Choi, W., Chong, Y. H., Hong, L., Kim, S.-H., Park, C.-W., and Kim, C.-G. (1994) Complete nucleotide sequence and tissue-specific expression of the rat phenylethanolamine N-methyltransferase gene. J. Neurochem. 63, 1603-1608.
15. 15. Morita, S., Kobayashi, K., Hidaka, H., and Nagatsu, T. (1992) Organization and complete nucleotide sequence of the gene encoding mouse phenylethanolamine N-methyltransferase. Mol. Brain. Res. 13, 313-319.
16. 16. Kitabchi, A. E., and Williams, R. H. (1969) Phenylethanolamine N-methyltransferase in human adrenal gland. Biochim. Biophys. Acta 178, 181-184.
17. 17. Studier, F. W., and Moffatt, B. A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
18. 18. Vieira, J., and Messing, J. (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259-268.
19. 19. Sasaoka, T., Kaneda, N., Kurosawa, Y., Fujita, K., and Nagatsu., T. (1989) Structure of human phenylethanolamine N-methyltransferase gene: Existence of two types of mRNA with different transcription initiation sites. Neurochem. Int. 15, 555-565.
20. 20. Sambrook, J., Fritsch, E. F., and Maniais, T. (1989) in "Molecular Cloning, A Laboratory Manual," Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
21. 21. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
22. 22. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein - dye binding. Anal. Biochem. 72, 248-254.
23. 23. Beaudouin, C., Haurat, G., Fraisse, L., Souppe, J., and Renaud, B. (1993) Assay of phenylethanolamine N-methyltransferase activity using high-performance liquid chromatography with ultraviolet absorbance detection. J. Chromatogr. 613, 51-58.
24. 24. Lee, H.-S., Schulz, A. R., and Fuller, R. W. (1978b) The interaction of rabbit adrenal norepinephrine N-methyltransferase isozymes with substrates. Arch. Biochim. Biophys. 185, 228-238.
25. 25. Wong, D. L., Yamasaki, L., and Ciaranello, R. D. (1987) Characterization of the isozymes of bovine adrenal medullary phenylethanolamine N-methyltransferase. Brain Research, 410, 32-44.
26. 26. Fujioka, M. (1992) Mammalian small molecule methyltransferases: Their structural and functional features. Int. J. Biochem. 24, 1917-1924.