Neurofilament M mRNA is expressed in conduction system myocytes of the developing and adult rabbit heart

Journal of Molecular and Cellular Cardiology

Volumn 28, Issue 9, 1996, Pages 1833-1844

  Vitadello, Maurizio ^a   Vettore, Silvia ^a   Lamar, Elise ^b   Chien, Kenneth R ^b   Gorza, Luisa ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

cDNA library; Heart conduction system; Heart development; In situ hybridization; Neurofilament M mRNA

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA;

ANIMAL TISSUE; ARTICLE; DNA LIBRARY; EMBRYO; HEART ATRIOVENTRICULAR NODE; HEART DEVELOPMENT; HEART MUSCLE CELL; HEART MUSCLE CONDUCTION SYSTEM; IN SITU HYBRIDIZATION; NEUROFILAMENT; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SINUS NODE;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0030249462     PISSN: 00222828     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmcc.1996.0176     Document Type: Article

References (29)

1. AOYAMA N, KIKAWADA R, YAMASHINA S, 1993. Immunohistochemical study on the development of the rat heart conduction system using anti-Leu-7 antibody. Arch Histol Cytol 56: 303-315.
2. +) RNA on oligo(dT) cellulose. In: Current Protocols in Molecular Biology, Section 11-3, Wiley: New York, 139-142.
3. BENNET GS, DILULLO C, 1985. Transient expression of a neurofilament protein by replicating neuroepithelial cells of the embryonic chick brain. Dev Biol 107: 107-127.
4. CARMELIET E, VEERECKE J, 1979. Electrogenesis of the action potential and automaticity. In: R.M. Berne, N. Sperelakis, and B.S. Geiger, eds. Handbook of Physiology, Section 2: The Cardiovascular System. American Physiological Society: Bethesda, 269-334.
5. CHOMCZYNSKI P, SACCHI N, 1987. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal Biochem 162: 156-159.
6. ESCURAT M, DJABALI K, HUC C, LANDON F, BECOURT C, BOITARD C, GROS F, PORTIER MM, 1991. Origin of the beta cells of the islets of Langerhans is further questioned by the expression of neuronal intermediate filament proteins, peripherin and NF-L, in the rat insulinoma R1N5 cell line. Dev Neurosci 13: 424-432.
7. FLIEGNER KH, CHING GY, LIEM RHK, 1990. The predicted amino acid sequence of α internexin is that of a novel neuronal intermediate filament protein. EMBO J 9: 749-755.
8. GEISLER N, FISCHER J, VANDEKERCKHOVE J, PLESSMANN U, WEBER K, 1984. Hybrid character of a large neurofilament protein (NF-M): intermediate filament type followed by a long and acidic carboxy-terminal extension. EMBO J 3: 2701-2706.
9. GORZA L, AUSONI S, MERCIAI N, HASTINGS KEM, SCHIAFFINO S, 1993. Regional differences in troponin I isoform switching during rat heart development. Dev Biol 156: 253-264.
10. GORZA L, SCHIAFFINO S, VITADELLO M, 1988. Heart conduction system: a neural crest derivative? Brain Res 457: 360-366.
11. GORZA L, VETTORE S, VITADELLO M, 1994. Molecular and cellular diversity of heart conduction system myocytes. Trend Cardiovasc Med 4: 153-158.
12. GORZA L, VITADELLO M, 1989. Distribution of conduction system fibers in the developing and adult rabbit heart revealed by an antineurofilament antibody. Circ Res 65: 360-369.
13. GOURDIE RG, MIMA T, THOMPSON RP, MIKAWA T, 1995. Terminal diversification of the myocyte lineage generates Purkinje fibers of the cardiac conduction system. Development 121: 1423-1431.
14. HOFFMANN PN, CLEVELAND DW, GRIFFIN JW, LANDES PW, COWAN NJ, PRICE DL, 1987. Neurofilament gene expression: a major determinant of axonal caliber. Proc Natl Acad Sci USA 84: 3472-3476.
15. KELLY BM, STEWART GILLESPIE C, SHERMAN DL, BROPHY PJ, 1992. Schwann cells of the myelin-forming phenotype express neurofilament protein NF-M. J Cell Biol 118: 397-410.
16. LEVY E, LIEM RKH, D'EUSTACHIO P, COWAN LC, 1987. Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular neurofilament protein. Eur J Biochem 166: 71-77.
17. MIERENDORF RC, PERCY C, YOUNG RA, 1987. Gene isolation by screening λgt 11 libraries with antibodies. Meth Enzymol 152: 458-469.
18. MYERS MW, LAZZARINI RA, LEE VM-Y, SCHLAEPFER WW, NELSON DL, 1987. The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family. EMBO J 6: 1617-1626.
19. NAPOLITANO EW, CHIN SSM, COLMAN DR, LIEM RHK, 1987. Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein. J Neurosci 7: 2590-2599.
20. SANGER F, NICKLEN F, COULSON AR, 1977. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
21. SECHRIST J, SERBEDZIJA GN, SCHERSON T, FRASER SE, BRONNER-FRASERM, 1993. Segmental migration of the hind-brain neural crest does not arise from its segmental generation. Development 118: 691-703.
22. SISSMAN NJ, 1970. Developmental landmarks in cardiac morphogenesis: comparative chronology. Am J Cardiol 25: 141-148.
23. STEINERT PM, ROOP DR, 1988. Molecular and cellular biology of intermediate filaments. Annu Rev Biochem 57: 593-625.
24. VITADELLO M, DENIS-DONINI S, 1990. Expression of neurofilament proteins in granule cells of the cerebellum. Brain Res 509: 47-54.
25. VITADELLO M, MATTEOLI M, GORZA L, 1990. Neurofilament proteins are co-expressed with desmin in heart conduction system myocytes. J Cell Sci 97: 11-21.
26. VITADELLO M, TRIBAN C, FABRIS M, GORIO A, SCHIAFFINO S, 1986. Heterogeneity of rat neurofilament polypeptides revealed by a monoclonal antibody. J Neurochem 46: 665-670.
27. WESSELS A, VERMEULEN JLM, VERBEEK FJ, VIRAGH SZ, KALMAN, LAMERS WH, MOORMAN, AFM, 1992. Spatial distribution of "tissue specific" antigens in the developing human heart and skeletal muscle. III. An immunohistochemical analysis of the distribution of the neural tissue antigen G1N2 in the embryonic heart; implications for the development of the atrio-ventricular conduction system. Anat Rec 232: 97-111.
28. ZHU L, LYONS GE, JUHASZ O, JOYA JE, HARDEMAN EC, WADE R, 1995. Developmental regulation of troponin I isoform genes in striated muscles of transgenic mice. Dev Biol 169: 487-503.
29. ZOPF D, DINEVA B, BETZ H, GUNDELFINGER ED, 1990. Isolation of the middle-molecular weight neurofilament (NF-M) gene and characterization of its promotor. Nucl Acid Res 118: 521-529.