Purification and characterization of human heart fatty acid ethyl eater synthase/carboxylesterase

Journal of Molecular and Cellular Cardiology

Volumn 28, Issue 9, 1996, Pages 2027-2032

  Bora, Puran S ^a   Guruge, Bandula L ^b   Miller, D Douglas ^a   Chaitman, Bernard R ^b   Ruyle, Matthew S ^a  

^a S LOUIS UNIV HLTH SCIENCES CENTER   (United States)

^b St Louis University Medical Center ^*

Author keywords

Alcohol; Carboxylesterase; Enzyme; Fatty acid ethyl ester synthase; Myocardium

Indexed keywords

ALCOHOL; CARBOXYLESTERASE; CROSS REACTING ANTIBODY; CYTOPLASM PROTEIN; ENZYME ANTIBODY; FATTY ACID ESTER; FATTY ACID SYNTHASE; HEART ENZYME; MICROSOME ENZYME; OLEIC ACID;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; FATTY ACID METABOLISM; HEART MUSCLE; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

EID: 0030249385     PISSN: 00222828     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmcc.1996.0195     Document Type: Article

References (23)

1. BORA P, BORA N, WU X, LANGE L, 1991. Molecular cloning, sequencing and expression of human myocardial fatty acid ethyl ester synthase-III cDNA. J Biol Chem 266: 16774-16777.
2. BORA P, LANGE L, 1991. Alcohol and Drug Abuse Reviews, Watson R.R., ed., Vol II, Humana Press Inc., Clifton N.J., 150-165.
3. BORA P, LANGE L, 1991. Fatty acid ethyl ester and heart disease. Alcohol Health Res World 14: 26-29.
4. BORA P, LANGE L, 1991. Homogenous synthase from human myocardium is a glutathione S-transferase. Proc New York Acad Sci USA 625: 827-829.
5. BORA P, LANGE L, 1993. Molecular mechanism of ethanol metabolism by human brain to fatty acid ethyl esters. Alcohol Clin Exp Res 19: 119-122.
6. BORA P, SPILBURG C, LANGE L, 1989a. Identification of a satellite fatty acid ethyl ester synthase from human myocardium as a glutathione S-transferase. J Clin Invest 84: 1942-1946.
7. BORA P, SPILBURG C, LANGE L, 1989b. Purification of homogeneity and characterization of major fatty acid ethyl ester synthase from human myocardium. FEBS Lett 258: 236-239.
8. BORA P, SPILBURG C, LANGE L, 1989c. Metabolism of ethanol and carcinogens by glutathione transferases. Proc Natl Acad Sci USA 86: 4470-4473.
9. BORA P, WU X, SPILBURG C, LANGE L, 1992. Purification and characterization of fatty acid ethyl ester synthase-II from human myocardium. J Biol Chem 267: 13217-13221.
10. BORA P, SMITH C, CLONINGER C, GERHARD D, 1994. Hinj polymorphism detected by FAEES-III cDNA. Hum Mol Genet 3: 2267-2268.
11. BORA P, MILLER D, CHAITMAN B, GURUGE B, 1996. Myocardial cell damage by fatty acid ethyl esters. J Cardiovasc Pharmacol 27: 1-6.
12. BORST P, LOOS J, CHRIST E, SLATER E, 1962. Uncoupling activity of long chain fatty acids. Biochem Biophys Acta 62: 509-517.
13. BRADFORD M, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-255.
14. CLONINGER C, BOHMAN M, SIGVARDSSON D, 1981. Inheritance of alcohol abuse. Cross-fostering analysis of adopted men. Arch Gen Psychiatry 38: 861-868.
15. GILLIGAN S, CLONINGER R, LANGE L, 1989. Segregation of fatty acid ethyl ester synthase gene. Am J Human Genet 46: 145-150.
16. GOODWIN D, 1987. Genetic factors in the development of alcoholism. Adv Intern Med 31: 383-398.
17. LANGE L, SOBEL B, 1983. Mitochondrial dysfunction induced by fatty acid ethyl esters, myocardial metabolites of ethanol. J Clin Invest 72: 724-731.
18. MENTLEIN R, HEYMANN E, 1984. Hydrolysis of ester and amide type drugs by the purified isoenzymes of nonspecific carbosylesterase from rat liver. Biochem Pharmacol 33: 1243-1248.
19. MENTLEIN R, 1986. The tumor promoter 12-0-tetradecanoyl phorbol 13 acetate and regulatory diacylglycerols are substrates for the same carboxylesterase. J Biol Chem 261: 7816-7818.
20. MOGELSON S, LANGE L, 1984. Non-oxidative ethanol metabolism in rabbit myocardium: purification to homogeneity of fatty acid ethyl ester synthase. Biochemistry 23: 4075-4081.
21. SEGAL L, REVDIG S, MASON D, 1979. Left ventricular dysfunction of isolated working rat hearts after chronic alcohol consumption. Cardiovasc Res 13: 126-146.
22. TSUJITA T, OKUDA H, 1992. Fatty acid ethyl ester synthase in rat adipose tissue and its relationship to carboxylesterase. J Biol Chem 267: 23489-23494.
23. TSUJITA T, OKUDA H, 1994. Fatty acid ethyl ester-synthesizing activity of lipoprotein lipase from rat post-heparin plasma. J Biol Chem 269: 5884-5889.