Detection of baculovirus gene expression in insect cells and larvae by low light video image analysis

Journal of Virological Methods

Volumn 61, Issue 1-2, 1996, Pages 151-156

  Langridge, W H R ^a   Krausova, V I ^b   Szalay, A A ^a   Fodor, I ^a,b,c  

^a LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b SKRYABIN INSTITUTE OF BIOCHEMISTRY AND PHYSIOLOGY OF MICROORGANISMS   (Russian Federation)

^c SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

Baculovirus; Chemiluminescence; Firefly luciferase; Recombinant DNA; Video image analysis

Indexed keywords

LUCIFERASE; RECOMBINANT DNA;

ARTICLE; BACULOVIRUS; BIOLUMINESCENCE; CHEMOLUMINESCENCE; CONTROLLED STUDY; GENE EXPRESSION; INSECT; LARVA; NONHUMAN; PRIORITY JOURNAL; VIDEORECORDING;

BRASSICA OLERACEA VAR. CAPITATA; INSECTA; LEPIDOPTERA; LYCOPERSICON ESCULENTUM; MANDUCA SEXTA; PHACELIA CONGESTA; TRICHOPLUSIA NI; UNIDENTIFIED BACULOVIRUS;

EID: 0030248805     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(96)02079-4     Document Type: Article

References (20)

1. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
2. de Wet, J.R., Wood, K.V., De Luca, M., Helinski, D.R. and Subramani, S. (1987). Firefly luciferase gene: structure and expressions in mammalian cells. Mol. Cel. Biol. 7, 725-737.
3. Engebrecht, J., Simon, M. and Silverman, M. (1985) Measuring gene expression with light. Science 227, 1345-1347.
4. Hasnain, S.E. and Nakhai, B. (1990) Expression of gene encoding firefly luciferase in insect cells using a baculovirus system. Gene 91, 135-138.
5. Kopylova-Sviridova, T.N., Gorelova, T.V., Krauzova, V.I., Timiryasova, T.M., Fodor, I. and Shuppe N.G. (1990) Baculovirus system of firefly luciferase expression in silk-worm insect cells. Dokl. Acad. Nauk SSSR 312, 1507-1510.
6. Kopylova-Sviridova, T.N., Krauzova, V.I., Timiryasova, T.M., Gorelova, T.V., Shuppe, N.G. and Fodor, I. (1992)Transient expression assay in a baculovirus system using firefly luciferase gene as a reporter. Virus Genes 6, 379-386.
7. Krausova, V.I., Kopylova-Sviridova, T.N., Timiryasova, T.M. and Fodor, I. (1991) Expression of firefly luciferase gene in mammalian cells using vaccinia virus vectors. Mol. Genet. Microbiol. Virol. (Moscow) 2, 23-28.
8. Langridge, W., Escher, A., Wang, G., Ayre, B., Fodor, I. and Szalay, A.A. (1994) Low-light image analysis of transgenic organisms using bacterial luciferase as a marker. J. Biolumin. Chemilumin. 9, 185-200.
9. Langridge, W., Jiang, C., Wang, G. et al. (1993) Use of a luciferase marker gene system to monitor gene expression in bacteria, plant and virus infected animal cells. In: A. Szalay, L.J. Kricka, and P. Stanley (Eds.), Bioluminescence and Chemoluminescence: Status Report. Wiley, Chichester, pp. 217-221.
10. Medin, JA, Hunt, L., Goeth, K., Evans, R.K. and Coleman, M.S. (1990) Efficient, low-cost protein factories: expression of human adenosine deaminase in baculovirus-infected insect larvae. Proc. Natl. Acad. Sci. USA. 87, 2760-2764.
11. Miller, L.K. (1988) Baculoviruses as gene expression vectors. Ann. Rev. Microbiol. 42, 177-199.
12. Mittal, S.K., McDermott, M.R., Johnson, D.C., Prevec, L. and Graham, F.L.(1993) Monitoring foreign gene expression by a human adenovirus-based vector using the firefly luciferase gene as a reporter. Virus Res. 28, 67-90.
13. Mori, H., Nakazawa, H., Shirai, N., Shibata, N., Sumida, M. and Matsubara, F. (1992) Foreign gene expression by baculovirus vector with an expanded host range. J. Gen. Virol. 73, 1877-1880.
14. Oker-Blom, C., Jansson, C., Karp, M. et al. (1993) Functional analysis of the human alpha 2C-C4 adrenergic receptor in insect cells expressed by a luciferase-based baculovirus vector. Biochim. Biophys. Acta 1176, 269-275.
15. Ow, D.W., Wood, K.V., DeLuca, M., de Wet, J.R., Helinski, D.R. and Howell, S.H. (1986) Transient and stable expression of firefly luciferase gene in plant cells and transgenic plants. Science 234, 856-859.
16. Price, P.M., Reichelderfer, C.F., Johanson, B.E., Kilbourne, E.D. and Acs, G. (1989) Complementation of recombinant baculoviruses by coinfection with wild-type virus facilitates production in insect larvae of antigenic proteins of hepatitis B virus and influenza virus. Proc. Natl. Acad. Sci. USA. 86, 1453-1456.
17. Richardson, C.D., Banville, M., Lalumiere, M., Vialard, J. and Meighen, E.A. (1992) Bacterial luciferase produced with rapid-screening baculovirus vectors is a sensitive reporter for infection of insect cells and larvae. Intervirology 34, 213-227.
18. Rodriguez, J.F., Rodriguez, D., Rodriguez, J-R., McGowan, E.B. and Esteban, M. (1988) Expression of the firefly luciferase gene in vaccinia virus: A highly sensitive gene marker to follow virus dissemination in tissues of infected animals. Proc. Natl. Acad. Sci. USA. 85, 1667-1671.
19. van den Heuvel, J.F., Thornbury, D.W., Pirone, T.P. and Lesnaw, J.A. (1993) A simple and efficient procedure forthe oral inoculation of Trichoplusia ni larvae with polyhedrin-negative recombinant baculovirus. J. Virol. Methods 42, 207-216.
20. Volkman, L.E. and Summers, M.D. (1976) Autographa californica nuclear polyhedrosis virus: comparative infectivity of the occluded, alkali-liberated, and nonoccluded forms. J. Invertebr. Pathol. 30, 102-103.