Characterization of composite membrane of poly(vinyl alcohol) and regenerated silk fibroin for immobilization of horseradish peroxidase and an amperometric neckelocene-mediated sensor sensitive to hydrogen peroxide

Journal of Chemical Technology and Biotechnology

Volumn 67, Issue 1, 1996, Pages 77-83

  Liu, Haiying ^a   Zhang, Xiaolin ^a   Zhang, Zhanen ^b   Qi, Deyao ^a   Fan, Yubo ^c   Liu, Yongcheng ^c   Liu, Shenghui ^c   Yu, Tongyin ^c   Deng, Jiaqi ^c  

^a SHANGHAI UNIVERSITY   (China)

^b Suzhou Inst City Constr E   (China)

^c FUDAN UNIVERSITY   (China)

Author keywords

Horseradish peroxidase; Hydrogen peroxide; Nickelocene; Poly(vinyl alcohol); Regenerated silk fibroin; Sensor

Indexed keywords

AMPEROMETRIC SENSORS; CHARACTERIZATION; ENZYME IMMOBILIZATION; HYDROGEN PEROXIDE; INFRARED SPECTROSCOPY; MORPHOLOGY; POLYVINYL ALCOHOLS; PROTEINS; SCANNING ELECTRON MICROSCOPY; SILK; VISUALIZATION;

AMPEROMETRIC NECKELOCENE MEDIATED SENSOR; COMPOSITE MEMBRANE; ELECTRON TRANSFER MEDIATOR; GLASSY CARBON ELECTRODE; HORSERADISH PEROXIDASE; REGENERATED SILK FIBROIN;

ENZYMES;

FIBROIN; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE; POLYVINYL ALCOHOL;

ARTICLE; ARTIFICIAL MEMBRANE; ELECTRODE; ELECTRON TRANSPORT; ENZYME IMMOBILIZATION; INFRARED SPECTROSCOPY; MEDIATOR; PH; SCANNING ELECTRON MICROSCOPY; SENSOR; SILK; TEMPERATURE SENSITIVITY;

EID: 0030248507     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4660(199609)67:1<77::AID-JCTB526>3.0.CO;2-4     Document Type: Article

References (30)

1. Ho, W. O., Athey, D. & McNeil, C. J., Amperometric detection of alkaline phosphatase activity at a horseradish peroxidase enzyme electrode based on activated carbon: potential application to electrochemical immunossay. Biosens. Bioelectron., 10 (1995) 683-91.
2. Oliver, J. M., Berlin, R. D. & Davis, B. H., Use of horseradish peroxidase and fluorescent dextrans to study fluid pinocytosis in leukocytes. Meth. Enzymol., 108 (1984) 336-47.
3. Renz, M. & Kurz, C., A colorimetric method for DNA hydridization. Nucl. Acids Res., 12 (1984) 3435-44.
4. Dordick, J. S., Enzymatic and chemoenzymatic approaches to polymer synthesis. Trends Biotechnol., 10 (1992) 287-93.
5. Takashashi, K., Nishimura, H., Yoshimoto, T., Saito, Y. & Inada, Y., A chemical modification to make horseradish peroxidase soluble and active in benzene. Biochem. Biophys. Res. Commun., 121 (1984) 261-5.
6. Dunford, H. B., Horseradish peroxidase: structure and kinetic properties. In Peroxidases in Chemistry and Biology. Vol. 2, eds J. Everse, K. E. Everse & M. B. Grisham. CRC Press, Boca Raton, FL, 1991, pp. 1-24.
7. Ruzgas, T., Gorton, L., Emneus, J. & Marko-Varga, G., Kinetic models of horseradish peroxidase action on a graphite electrode. J. Electroanal. Chem., 391 (1995) 41-9.
8. Tatsuma, T., Ariyama, K. & Oyama, N., Electron transfer from a polythiophene derivative to compounds I and II of peroxidases. Anal. Chem., 67 (1995) 283-7.
9. Gorton, L., Jonsson-Pettersson, G., Csoregi, E., Johansson, K., Dominguez, E. & Marko-Varga, G., Amperometric biosensors based on an apparent direct electron transfer between electrodes and immobilized peroxidases. Analyst, 117 (1992) 1235-41.
10. Smith, A. T., Sanders, S. A., Greschik, H., Thorneley, R. N. F., Burke, J. F. & Bray, A. C., Probing the mechanism of horseradish peroxidase by site-directed mutagenesis. Biochem. Soc. Transact., 20 (1992) 340-45.
11. 2. Biosens. Bioelectron., 10 (1995) 443-61.
12. Wang, J., Freiha, B., Naser, N., Romero, E. G., Wollenberger, U. & Ozsoz, M., Amperometric biosensing of organic peroxides with peroxidase-modified electrodes. Anal. Chim. Acta 254 (1991) 81-8.
13. Wollenberger, B., Wang, J., Ozsoz, M., Romero, E. G. & Scheller, F., Bulk modified enzyme electrodes for reagentless detection of peroxides. Bioelectrochem. & Bioenerget., 26 (1991) 287-96.
14. Ho, W. O., Athey, D., McNeil, C. J., Hager, H. J., Evans, G. P. & Mullen, W. H., Mediatorless horseradish peroxidase enzyme electrodes based on activated carbon: potential application to specific binding assay. J. Electroanal. Chem., 351 (1993) 185-97.
15. Zhao, J., Henkens, R. W., Stonehuemer, J., O'Daly, J. P. & Crumbliss, A. L., Direct electron transfer at horseradish peroxidase-colloidal gold modified electrodes. J. Electroanal. Chem., 327 (1992) 109-19.
16. Csoregl, E., Gorton, L., Marko-Varga, G., Tudos, A. J. & Kok, W. T., Peroxidase-modified carbon fiber microelectrodes in flow-through detection of hydrogen peroxide and organic peroxides. Anal. Chem., 66 (1994) 3604-10.
17. Tatsuma, T., Gondaira, M. & Watanabe, T., Peroxidase-incorporated polypyrrole membrane electrodes. Anal. Chem., 64 (1992) 1183-7.
18. Deng, Q. & Dong, S., Mediatorless hydrogen peroxide electrode based on horseradish peroxidase entrapped in poly(o-phenylenediamine). J. Electroanal. Chem., 377 (1994) 191-5.
19. Frew, J. E., Harmer, M. A., Hill, H. A. O. & Libor, S. I., A method for estimation of hydrogen peroxide based on mediated electron transfer reactions of peroxidases at electrodes. J. Electroanal. Chem., 201 (1986) 1-10.
20. Garguilo, M. G., Huynh, N., Proctor, A. & Michael, A. C., Amperometric sensors for peroxide, choline and acetylcholine based on electron transfer between horseradish peroxidase and a redox polymer. Anal. Chem., 65 (1993) 523-8.
21. Mulchandani, A., Wang, C. H. & Weetall, H. H., Amperometric detection of peroxides with poly(anilinomethylferrocene)-modified enzyme electrodes. Anal. Chem., 67 (1995) 94-100.
22. Adeyoju, C., Iwuoha, E. I. & Smyth, M. R., Reactivities of amperometric organic phase peroxidase-modified electrodes in the presence and absence of thiourea and ethylenethiourea as inhibitors. Anal. Chim. Acta, 305 (1995) 57-64.
23. Bifulco, L., Cammaroto, C., Newman, J. D. & Turner, A. P. F., TTF-modified biosensors for hydrogen peroxide. Anal. Lett., 27(8) (1994) 1443-52.
24. Vreeke, M., Rocca, P. & Heller, A., Direct electrical detection of dissolved biotinylated horseradish peroxidase, biotin and avidin. Anal. Chem., 67 (1995) 303-6.
25. Yang, L., Janle, E., Huang, T., Gitzen, J., Kissinger, P. T., Vreeke, M. & Heller, A., Applications of 'wired' peroxidase electrodes for peroxide determination in liquid chromatography coupled to oxidase immobilized enzyme reactors. Anal. Chem., 67 (1995) 1326-31.
26. 2 sensor for organic-phase assays based on regenerated silk fibroin as immobilization matrix for peroxidase. Electrochim. Acta, 41 (1996) 77-82.
27. Tsukada, M., Freddi, G., Gotoh, Y. & Kasai, N., Physical and chemical properties of tussah silk fibroin films. J. Polymer Science: Part B: Polymer Physics, 32 (1994) 1407-12.
28. Asakura, T. & Demura, M., Immobilization of glucose oxidase with Bombyx mori silk fibroin by only stretching treatment and its application to glucose sensor. Biotechnology and Bioengineering, 33 (1989) 598-603.
29. Demura, M., Takekawa, T., Asakura, T. & Nishikawa, A., Characterization of low-temperature-plasma treated silk fibroin fabrics by ESCA and the use of the fabrics as an enzyme-immobilization support. Biomaterials, 13 (1992) 276-81.
30. Liu, Y., Liu, H., Qian, H., Deng, J. & Yu, T., Immobilization of glucose oxidase in the regenerated silk fibroin membrane: characterisation of the membrane structure and its application to an amperometric glucose sensor employing ferrocene as electron shuttle. Journal of Chemical Technology and Biotechnology, 64 (1995) 269-76.