Purification and characterization of LasR as a DNA-binding protein

FEMS Microbiology Letters

Volumn 142, Issue 2-3, 1996, Pages 301-307

  You, Zhiying ^a   Fukushima, Jun ^a   Ishiwata, Tetsuyoshi ^a   Chang, Baotong ^a   Kurata, Minoru ^a   Kawamoto, Susumu ^a   Williams, Paul ^b   Okuda, Kenji ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Autoinducer; DNA binding protein; LasB gene; LasR; Pseudomonas aeruginosa; Transcriptional activator

Indexed keywords

DNA BINDING PROTEIN;

ARTICLE; CONTROLLED STUDY; DNA BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA; TRANSCRIPTION REGULATION;

EID: 0030245619     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1097(96)00286-8     Document Type: Article

References (20)

1. [1] Meighen, E.A. (1993) Bacterial bioluminescence: organization, regulation, and application of the lux genes. FASEB. J. 7, 1016-1022.
2. [2] Gambello, M.J. and Iglewski, B.H. (1991) Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J. Bacteriol. 173, 3000-3009.
3. [3] Morihara, K. and Homma, J.Y. (1985) Pseudomonas protease. In: Bacterial Enzymes and Virulence (Holder, I.A., Ed.), pp. 41-79, CRC Press, Boca Raton, FL.
4. [4] Bever, R.A. and Iglewski, B.H. (1988) Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene. J. Bacteriol. 170, 4309-4314.
5. [5] Fukushima, J., Yamamoto, S., Morihala, K., Atsumi, Y., Takeuchi, H., Kawamoto, S. and Okuda, K. (1989) Structural gene and complete amino acid sequence of Pseudomonas aeruginosa IFO3455 elastase. J. Bacteriol. 171, 1698-1704.
6. [6] Yamamoto, S., Fukusima, J., Atsumi, Y., Takeuchi, H., Kawamoto, S., Okuda, K. and Morihara, K. (1988) Cloning and characterization of elastase structural gene from Pseudomonas aeruginosa IFO3455. Biochem. Biophys. Res. Commun. 152, 1117-1122.
7. [7] Passador, L., Cook, J.M., Gambello, M.J., Rust, L. and Iglewski., B.H. (1993) Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science 260, 1127-1130.
8. [8] Pearson, J.P., Gray, K.M., Passador, L., Tucker, K.D., Eberhard, A., Iglewski, B.H. and Greenberg, E.P. (1994) Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proc. Natl. Acad. Sci. USA 91, 197-201.
9. [9] Choi, S.H. and Greenberg, E.P. (1992) Genetic evidence for multimerization of LuxR, the transcriptional activator of Vibrio fischeri luminescence. Mol. Mar. Biol. Biotechnol. 1, 408-413.
10. [10] Fuqua, W.C., Winans, S.C. and Greenberg, E.P. (1994) Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J. Bacteriol. 176, 269-275.
11. [11] Gray, K.M., Passador, L., Iglewski, B.H. and Greenberg, E.P. (1994) Interchangeability and specificity of components from the quorum-sensing regulatory systems of Vibrio fischeri and Pesudomonas aeruginosa. J. Bacteriol. 176, 3076-3080.
12. [12] Chhabra, S.R., Stead, P., Bainton, N.J., Salmond, G.P.C., Stewart, G.S.A.B., Williams, P. and Bycroft, B.W. (1993) Autoregulation of carbapenem biosynthesis in Erwinia carotovora by analogues of N-(3-oxohexanoyl)-L-homoserine lactone. J. Antibiot. 46, 441-449.
13. [13] Rust, L., Pesci, E.G. and Iglewski, B.H. (1996) Analysis of the Pseudomonas aeruginosa elastase (lasB) regulatory region. J. Bacteriol. 178, 1134-1140.
14. [14] Fried, M. and Crothers, D.M. (1981) Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 9, 6505-6525.
15. [15] Shinagawa, H., Iwasaki, H., Kato, T. and Nakata, A. (1988) RecA protein dependent cleavage of UmuD protein and SOS mutagensis. Proc. Natl. Acad. Sci. USA 85, 1806-1810.
16. [16] Nardelly, B., Lu, Y.A., Shiu, D.R., Delpierre-Defoort, C., Proty, A.T. and Tam, J.P. (1992) A chemically defined synthetic vaccine model for HIV-1. J. Immunol. 148, 914-920.
17. [17] Fukushima, J., Ishiwata, T., Kurata, M., You, Z.Y. and Okuda, K. (1994) Intracellular receptor-type transcription factor, LasR, contains a highly conserved amphipathic region which precedes the putative helix-turn-helix DNA binding motif. Nucleic Acids Res. 22, 3706-3707.
18. [18] Bainton, N.J., Bycroft, B.W., Chhabra, S.R., Stead, P., Gledhill, L., Hill, P.J., Rees, C.E.D., Winson, M.K., Salmond, G.P.C., Stewart, G.S.A.B. and Williams, P. (1993) A general role for the lux autoinducer in bacterial cell signalling: control of antibiotic biosynthesis in Erwinia. Gene 116, 87-92.
19. [19] Miyamoto, C.M., Smith, E.E., Swartzman, E., Cao, J.G., Graham, A.F. and Meighen, E.A. (1994) Proximal and distalsites bind LuxR independently and activate expression of the Vibrio harveyi lux operon. Mol. Microbiol. 14, 255-262.
20. [20] Stevens, A.M., Dolan, K.M. and Greenberg, E.P. (1994) Synergistic binding of the Vibrio fischeri LuxR transcriptional activator domain and RNA polymerase to the lux promoter region. Proc. Natl. Acad. Sci. USA 91, 12619-12623.