Contribution of Heavy Chain Junctional Amino Acid Diversity to Antibody Affinity among p-Azophenylarsonate-Specific Antibodies

Journal of Immunology

Volumn 157, Issue 5, 1996, Pages 2066-2072

  Parhami Seren, Behnaz ^a   Margolies, Michael N ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 AZOBENZENEARSONIC ACID; AMINO ACID; IMMUNOGLOBULIN HEAVY CHAIN;

A J MOUSE; AMINO ACID SEQUENCE; ANIMAL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; ARTICLE; CHEMISTRY; GENETICS; IMMUNOGLOBULIN STRUCTURE; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; MOUSE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; ANTIBODY AFFINITY; ANTIBODY DIVERSITY; ANTIBODY SPECIFICITY; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN JOINING REGION; IMMUNOGLOBULIN VARIABLE REGION; MICE; MICE, INBRED A; MUTAGENESIS, SITE-DIRECTED; P-AZOBENZENEARSONATE;

EID: 0030240572     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (44)

1. Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature 302:575.
2. Honjo, T. 1983. Immunoglobulin genes. Annu. Rev. Immunol. 1:499.
3. Yancopoulous, G. D., and F. W. Alt. 1986. Regulation of the assembly and expression of variable-region genes. Annu. Rev. Immunol. 4:339.
4. Möller, G., ed. 1987. Role of somatic mutation in the generation of lymphocyte diversity. Immunol. Rev. 96:1.
5. McKean, D., K. Huppi, M. Bell, L. Staudt, W. Gerhard, and M. Weigert. 1984. Generation of antibody diversity in the immune response of BALB/c mice to influenza virus hemagglutinin. Proc. Natl. Acad. Sci. USA 81:3180.
6. Shlomchik, M. J., A. H. Aucoin, D. S. Pisetsky, and M. G. Weigert. 1987. Structure and function of anti DNA autoantibodies derived from a single autoimmune mouse. Proc. Natl. Acad. Sci. USA 84:9150.
7. Allen, D., A. Cumano, R. Dildrop, C. Kocks, K. Rajewsky, N. Rajewsky, J. Roes, F. Sablitzky, and M. Siekevitz. 1987. Timing, genetic requirements and functional consequences of somatic hypermutation during B-cell development. Immunol. Rev. 96:5.
8. Berek, C., and C. Milstein. 1987. Mutation drift and repertoire shift in the maturation of the immune response. Immunol. Rev. 96:23.
9. Sharon, J., M. L. Gefter, L. J. Wysocki, and M. N. Margolies. 1989. Recurrent somatic mutations in mouse antibodies to p-azophenylarsonate increase affinity for hapten. J. Immunol. 142:596.
10. Kuettner, M. G., A. L. Wang, and A. Nisonoff. 1972. Quantitative investigations of idiotypic antibodies. VI. Idiotypic specificity as a potential genetic marker for variable regions of mouse immunoglobulin polypeptide chains. J. Exp. Med. 135: 579.
11. Siekevitz, M., M. L. Gefter, P. Brodeur, R. Riblet, and A. Marshak-Rothstein. 1982. The genetic basis of antibody production: the dominant anti-arsonate idiotype response of the strain A mouse. Eur. J. Immunol. 12:1023.
12. Siekevitz, M., S. Y. Huang, and M. L. Gefter. 1983. The genetic basis of antibody production: a single heavy chain variable region gene encodes all molecules bearing the dominant anti-arsonate idiotype in the strain A mouse. Eur. J. Immunol. 13:123.
13. H segment employed in Ars-A antibodies: implications for the generation of junctional diversity. J. Immunol. 137:362.
14. κ genes encode predominating antibody variable regions elicited in strain A mice by immunization with p-azophenylarsonate. J. Exp. Med. 166:1.
15. Jeske, D. J., J. Jarvis, C. Milstein, and J. D. Capra. 1984. Junctional diversity is essential to antibody activity. J. Immunol. 133:1090.
16. Wysocki, L. J., T. Manser, T. Gridley, and M. L. Gefter. 1986. Molecular limitations on variable-gene junctional diversity. J. Immunol. 137:3699.
17. Milner, E. C. D., K. D. Meek, G. Rathbun, P. Tucker, and J. D. Capra. 1986. Are anti-arsonate antibody N-segments selected at both the protein and DNA level? Immunol. Today 7:36.
18. Sharon, J., M. L. Gefter, T. Manser, and M. Ptashne. 1986. Site-directed mutagenesis of an invariant amino acid residue at the variable-diversity segments junction of an antibody. Proc. Natl. Acad. Sci. USA 83:2628.
19. H junctions affect the affinity of anti-p-azophenylarsonate antibodies. J. Immunol. 143:4090.
20. Strong, R. K., R. Campbell, D. R. Rose, G. A. Petsko, J. Sharon, and M. N. Margolies. 1991. Three-dimensional structure of the murine anti-p-azophenylarsonate Fab 36-71. 1. X-ray crystallography, site-directed mutagenesis, and modeling of the complex with hapten. Biochemistry 30:3739.
21. Manser, T. 1990. Limits on heavy chain junctional diversity contribute to the recurrence of an antibody variable region. Mol. Immunol. 27:503.
22. Giusti, A. M., and T. Manser. 1993. Hypermutation is observed only in antibody H-chain V-region transgenes that have recombined with endogenous immunoglobulin H DNA: implications for the location of cis-acting elements required for somatic mutation. J. Exp. Med. 177:797.
23. Vora, K. A., and T. Manser. Altering the antibody repertoire via transgene homologous recombination: evidence for global and clone-autonomous regulation of antigen-driven B cell differentiation. 1995. J. Exp. Med. 181:271.
24. Marshak-Rothstein, A., M. Siekevitz, M. N. Margolies, M. Mudgett-Hunter, and M. L. Gefter. 1980. Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice. Proc. Natl. Acad. Sci. USA 77: 1120.
25. κ chimaeric protein in mouse myeloma cells. Nature 309:364.
26. Sharon, J. 1990. Structural correlates of high antibody affinity: three engineered amino-acid substitutions can increase the affinity of an anti-p-azophenylarsonate antibody 200-fold. Proc. Natl. Acad. Sci. USA 87:4814.
27. Sompuram, S. R., and J. Sharon. 1993. Verification of a model of a Fab-complex with phenylarsonate by oligonucleotide-directed mutagenesis. J. Immunol. 150: 1822.
28. Parhami-Seren, B., J. Sharon, and M. N. Margolies. 1990. Structural characterization of H chain-associated idiotopes of anti-p-azophenylarsonate monoclonal antibodies. J. Immunol. 144:4426.
29. Parhami-Seren, B., P. H. Kussie, R. K. Strong, and M. N. Margolies. 1993. Conservation of binding site geometry among p-azophenylarsonate-specific antibodies. J. Immunol. 150:1829.
30. Kussie, P. H., B. Parhami-Seren, L. J. Wysocki, and M. N. Margolies. 1994. A single engineered amino acid substitution changes antibody fine specificity. J. Immunol. 152:146.
31. Fish, S., M. Fleming, J. Sharon, and T. Manser. 1991. Different epitope structures select distinct mutant forms of an antibody variable region for expression during the immune response. J. Exp. Med. 173:665.
32. Manser, T. 1989. Evolution of antibody structure during the immune response: the differentiative potential of a single B lymphocyte. J. Exp. Med. 170:1211..
33. H gene segment: influence on the expression of a major cross-reactive idiotype. J. Immunol. 134:1236.
34. Strong, R. K., G. A. Petsko, J. Sharon, and M. N. Margolies. 1991. Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy. Biochemistry 30:3749.
35. Manser, T., L. J. Wysocki, T. Gridley, R. I. Near, and M. L. Gefter. 1985. The molecular evolution of the immune response. Immunol. Today 6:94.
36. Ellenberger, J., G. Creadon, X. Zhang, and L. J. Wysocki. 1993. Recruiting memory B cells with changed antigenic specificity. J. Immunol. 151:5272.37. Berek, C., A. Berger, and M. Apel. 1991. Maturation of the immune response in germinal centers. Cell 67:1121 .
37. Ellenberger, J., G. Creadon, X. Zhang, and L. J. Wysocki. 1993. Recruiting memory B cells with changed antigenic specificity. J. Immunol. 151:5272.37. Berek, C., A. Berger, and M. Apel. 1991. Maturation of the immune response in germinal centers. Cell 67:1121 .
38. Berek, C., A. Berger, and M. Apel. 1991. Maturation of the immune response in germinal centers. Cell 67:1121.
39. Jacob, J., G. Kelsoe, K. Rajewsky, and U. Weiss. 1991. Intraclonal generation of antibody mutants in germinal centers. Nature 354:389.
40. Apel, M., and C. Berek. 1991. Differentiation of B-cells in germinal centers. In Lymphatic Tissues and In Vivo Immune Responses. S. Izine, S. Berrih-Aknin, and B. Imhof, eds. Marcel Dekker Inc., New York, pp. 355-359.
41. Feeney, A. J., S. H. Clarke, and D. E. Mosier. 1988. Specific H chain junctional diversity may be required for non-T15 antibodies to bind phosphorylcholine. J. Immunol. 41:1267.
42. Wong, Y. W., P H. Kussie, B. Parhami-Seren, and M. N. Margolies. 1995. Modulation of antibody affinity by an engineered amino acid substitution. J. Immunol. 154:3351.
43. Fish, S., and T. Manser. 1987. Influence of the macromolecular form of a B cell epitope on the expression of antibody variable and constant region structure. J. Exp. Med. 166:711.
44. Kabat, E. A., M. Reid-Miller, H. M. Perry, and K. S. Gottesman. 1991. Sequences of Proteins of Immunological Interest, 5th Edition. National Institutes of Health, U.S. Department of Health and Human Services, Washington, DC.