Intraepithelial Lymphocytes in Human Gut Have Lytic Potential and a Cytokine Profile That Suggest T Helper 1 and Cytotoxic Functions

Journal of Immunology

Volumn 157, Issue 5, 1996, Pages 1926-1934

  Lundqvist, Carina ^a   Melgar, Silvia ^a   Yeung, Moorix Mo Wai ^a   Hammarström, Sten ^a   Hammarström, Marie Louise ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 1; INTERLEUKIN 10; INTERLEUKIN 2; INTERLEUKIN 8; LECTIN; LYMPHOCYTE ANTIGEN RECEPTOR; LYMPHOTOXIN; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA;

ADULT; AGED; ARTICLE; BIOSYNTHESIS; CELL CULTURE; COLON; CYTOLOGY; CYTOTOXIC T LYMPHOCYTE; CYTOTOXICITY; EPITHELIUM; EPITHELIUM CELL; FEMALE; GENETICS; HUMAN; ILEUM; IMMUNOLOGY; INTESTINE MUCOSA; JEJUNUM; MALE; METABOLISM; MIDDLE AGED; SECRETION; TH1 CELL;

ADULT; AGED; AGED, 80 AND OVER; CELLS, CULTURED; COLON; CYTOKINES; CYTOTOXICITY, IMMUNOLOGIC; EPITHELIAL CELLS; EPITHELIUM; FEMALE; HUMANS; ILEUM; INTERFERON TYPE II; INTERLEUKIN-1; INTERLEUKIN-10; INTERLEUKIN-2; INTERLEUKIN-8; INTESTINAL MUCOSA; JEJUNUM; LECTINS; LYMPHOTOXIN-ALPHA; MALE; MIDDLE AGED; RECEPTORS, ANTIGEN, T-CELL; RNA, MESSENGER; T-LYMPHOCYTES, CYTOTOXIC; TH1 CELLS; TRANSFORMING GROWTH FACTOR BETA; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0030239819     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (50)

1. Janeway, C. A., Jr., B. J. Jones, and A. Hayday. 1988. Specificity and function of T cells bearing γδ receptors. Immunol. Today 9:73.
2. Boismenu, R., and W. L. Havran. 1994. Modulation of epithelial cell growth by intraepithelial γδ T cells. Science 266:1253.
3. +,γδ TCR T cells produce IFN-γ and IL-5. J. Immunol. 147:3736.
4. + intraepithelial lymphocytes. Science 243:1716.
5. Kawaguchi, M., M. Nanno, Y. Umesaki, S. Matsumoto, Y. Okada, Z. Cai, T. Shimamura, Y. Matsuoka, M. Ohwaki, and H. Ishikawa. 1993. Cytolytic activity of intestinal intraepithelial lymphocytes in germ-free mice is strain dependent and determined by T cells expressing γδ T-cell antigen receptors. Proc. Natl. Acad. Sci. USA 90:8591.
6. Sydora, B. C., P. F. Mixter, H. R. Holcombe, P. Eghtesady, K. Williams, M. C. Amaral, A. Nel, and M. Kronenberg. 1993. Intestinal intraepithelial lymphocytes are activated and cytolytic but do not proliferate as well as other T cells in response to mitogenic signals. J. Immunol. 150:2179.
7. Yamamoto, S., F. Ruses, H. C. Teixeira, P. Conradt, and S. H. E. Kaufmann. 1993. Listeria monocytogenes-induced gamma interferon secretion by intestinal intraepithelial γ/δ T lymphocytes. Infect. Immun. 61:2154.
8. Tank, J., A. I. Robbers, and E. C. Ebert. 1992. Spontaneous cytotoxicity of human intraepithelial lymphocytes against epithelial cell tumors. Gastroenterology 102: 69.
9. Trejdosiewicz, L. K., C. J. Smart, D. J. Oakes, P. D. Howdle, G. Malizia, D. Campana, and A. W. Boylston. 1989. Expression of T-cell receptors TcR1 (α/δ) and TcR2 (α/β) in the human intestinal mucosa. Immunology 68:7.
10. Halstensen, T. S., and P. Brandtzaeg. 1993. Phenotypic characteristics of human intraepithelial lymphocytes. In Mucosal Immunology: Intraepithelial Lymphocytes. H. Kiyono and J. R. McGhee, eds. Raven Press Ltd., New York, p. 147.
11. Lundqvist, C., V. Baranov, S. Hammarström, L. Athlin, and M.-L. Hammarström. 1995. Intraepithelial lymphocytes: evidence for regional specialization and extrathymic T cell maturation in the human gut epithelium. Int. Immunol. 7:1473.
12. Camerini, V., C. Panwala, and M. Kronenberg. 1993. Regional specialization of the mucosal immune system: intraepithelial lymphocytes of the large intestine have a different phenotype and function than those of the small intestine. J. Immunol. 151:1765.
13. Lefrancois, L. 1991. Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire? Immunol. Today 12:436.
14. Bandeira, A., S. Itohara, M. Bonneville, O. Burlen-DeFranoux, T. Mota-Santos, A. Couthino, and S. Tonegawa. 1991. Extrathymic origin of intestinal intraepithelial lymphocytes bearing T-cell antigen receptor γδ. Proc. Natl. Acad. Sci. USA 88:43.
15. Rocha, B., H. von Boehmer, and D. Guy-Grand. 1992. Selection of intraepithelial lymphocytes with CD8 α/α co-receptors by self-antigen in the murine gut. Proc. Natl. Acad. Sci. USA 89:5336.
16. + cells from murine large intestine: implications for functional state. J. Exp. Med. 180:433.
17. Friedman, A., A. Al-Sabbagh, L. M. B. Santos, J. Fishman-Lobell, M. Polanski, M. P. Das, S. J. Khoury, and H. L. Weiner. 1994. Oral tolerance: a biologically relevant pathway to generate peripheral tolerance against external and self antigens. In Mechanisms of Immune Regulation, Vol. 58. R. D. Granstein, ed. Karger, Postfach, Basel, Switzerland, p. 259.
18. Mowat, A. M. 1987. The regulation of immune responses to dietary protein antigens. Immunol. Today 8:93.
19. Berzins, T., M. Vargas-Cortes, M.-L. Hammarsiröm, Å. Larsson, M. Aguilar-Santelises, G. Andersson, S. Hammarström, and P. Perlmann. 1988. Monoclonal antibodies against leucoagglutinin-reactive human T-lymphocyte surface components. Scand. J. Immunol. 28:773.
20. Lundqvist, C., M.-L. Hammarström, L. Athlin, and S. Hammarström. 1992. Isolation of functionally active intraepithelial lymphocytes and enterocytes from human small and large intestine. J. Immunol. Methods 152:253.
21. Lea, T., E. Smeland, S. Funderud, F. Vartdal, C. Davies, K. Beiske, and J. Ugelstad. 1986. Characterization of human mononuclear cells after positive selection with immunomagnetic particles. Scand. J. Immunol. 23:509.
22. Lundqvist, C., V. Baranov, S. Teglund, S. Hammarström, and M.-L. Hammarström. 1994. Cytokine profile and ultrastructure of intraepithelial γδ T cells in chronically inflamed human gingiva suggest a cytotoxic effector function. J. Immunol. 153:2302.
23. Dillner-Centerlind, M.-L., B. Axelsson, S. Hammarstrom, U. Hellström, and P. Perlmann. 1980. Interaction of lectins with human T lymphocytes: mitogenic properties, inhibitory effects, binding to the cell membrane and to isolated surface glycopeptides. Eur. J. Immunol. 10:434.
24. Andersson, G., H. T. P. Ekre, G. Alm, and P. Perlmann. 1989. Monoclonal antibody two-site ELISA for human IFN-γ. J. Immunol. Methods 125:89.
25. Mincheva-Nilsson, L., M.-L. Hammarström, P. Juto, and S. Hammarström. 1990. Human milk contains proteins that stimulate and suppress T lymphocyte proliferation. Clin. Exp. Immunol. 79:463.
26. Leo, O., D. H. Sachs, L. E. Samelson, M. Foo, R. Quinones, R. Gress, and J. A. Bluestone. 1986. Identification of monoclonal antibodies specific for the T cell receptor complex by Fc receptor-mediated CTL lysis. J. Immunol. 137:3874.
27. Ito, M., O. Usuba, J. C. Unkeless, R. Schreiber, F. Celada, C. A. Bona, and T. M. Moran. 1989. Sideways killing: the cytolysis of Fc receptor-bearing cells through bridging to cytolytic T lymphocytes by antibodies specific for the T-cell receptor-T3 complex. Scand. J. Immunol. 29:659.
28. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156.
29. Yamamura, M., K. Uyemura, R. J. Deans, K. Weinberg, T. H. Rea, and B. R. Bloom. 1991. Defining protective responses to pathogens: cytokine profiles in leprosy lesions. Science 254:277.
30. Ebert, E. 1989. Proliferative responses of human intraepithelial lymphocytes to various T-cell stimuli. Gastroenterology 97:1372.
31. Cerf-Bensussan, N., A. Quaroni, J. T. Kumick, and A. K. Bhan. 1984. Intraepithelial lymphocytes modulate Ia expression by intestinal epithelial cells. J. Immunol. 132:2244.
32. Greenwood, J., L. Austin, and W. Dobbins III. 1983. In vitro characterization of human intestinal intraepithelial lymphocytes. Gastroenterology 85:1023.
33. 7-containing integrin defined by HML-1 monoclonal antibody. Eur. J. Immunol. 22:2887.
34. Barrett, T. A., T. F. Gajewski, D. Danielpour, E. B. Chang, K. W. Beagley, and J. A. Bluestone. 1992. Differential function of intestinal intraepithelial lymphocyte subsets. J. Immunol. 149:1124.
35. + T cells via T cell receptor-CD3 complex results in interferon-γ and interleukin-5 production, while low density T cells undergo DNA fragmentation. Eur. J. Immunol. 24:1301.
36. Wilkinson, P. C., and I. Newman. 1992. Identification of IL-8 as a locomotor attractant for activated human lymphocytes in mononuclear cell cultures with anti-CD3 or purified protein derivative of Mycobacterium tuberculosis. J. Immunol. 149:2689.
37. + lymphocytes in response to attractants and mitogens. Immunology 78:92.
38. + intraepithelial lymphocytes in the murine small intestine. Immunology 86:212.
39. + T cells that secrete IFN-γ and IL-5. J. Immunol. 145:68.
40. Del Prete, G., M. De Carli, F. Almerigogna, M. Grazia Giudizi, R. Biagiotti, and S. Romagnani. 1993. Human IL-10 is produced by both type 1 helper (Th1) and type 2 helper (Th2) T cell clones and inhibits their antigen-specific proliferation and cytokine production. J. Immunol. 150:353.
41. Wahl, S. M., D. A. Hunt, H. L. Wong, S. Dougherty, N. McCarney-Francis, L. M. Wahl, L. Ellingsworth, J. A. Schmidt, G. Hall, A. B. Roberts, and M. B. Sporn. 1988. Transforming growth factor-β is a potent immunosuppressive agent that inhibits IL-1-dependent lymphocyte proliferation. J. Immunol. 140:3026.
42. Kim, P.-H., and M. F. Kagnoff. 1990. Transforming growth factor β1 increases IgA isotype switching at the clonal level. J. Immunol. 145:3773.
43. Defrance, T., B. Vanbervliet, F. Briere, I. Durand, F. Rousset, and J. Banchereau. 1992. Interleukin 10 and transforming growth factor β cooperate to induce anti-CD40-activated naive human B cells to secrete immunoglobulin A. J. Exp. Med. 175:671.
44. Mayer, L., and R. Shlien. 1987. Evidence for function of Ia molecules on gut epithelial cells in man. J. Exp. Med. 166:1471.
45. Bland, P. W., and L. G. Warren. 1986. Antigen presentation by epithelial cells of the rat small intestine. II. Selective induction of suppressor T cells. Immunology 58:9.
46. Panja, A., E. Siden, and L. Mayer. 1995. Synthesis and regulation of accessory/ proinflammatory cytokines by intestinal epithelial cells. Clin. Exp. Immunol. 100: 298.
47. McGee, D. W., T. Bamberg, S. J. D. Vitkus, and J. R. McGhee. 1995. A synergistic relationship between TNF-α, IL-1β, and TGF-β1 on IL-6 secretion by the IEC-6 intestinal epithelial cell line. Immunology 86:6.
48. Schuerer-Maly, C.-C., L. Eckmann, M. F. Kagnoff, M. T. Falco, and F.-E. Maly. 1994. Colonic epithelial cell lines as a source of interleukin-8: stimulation by inflammatory cytokines and bacterial lipopolysaccharide. Immunology 81:5.
49. Appasamy, P., T. W. Kenniston, Jr., Y. Weng, E. C. Holt, J. Kost, and W. H. Chambers. 1993. Interleukin 7-induced expression of specific T cell receptor y variable region genes in murine fetal liver cultures. J. Exp. Med. 178:2201.
50. Watanabe, M., Y. Ueno, T. Yajima, Y. Iwao, M. Tsuchiya, H. Ishikawa, S. Aiso, T. Hibi, and H. Ishii. 1995. Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of intestinal mucosal lymphocytes. J. Clin. Invest. 95:2945.