Improvements to the TMSBr method of peptide resin deprotection and cleavage: Application to large peptides

Peptide Research

Volumn 9, Issue 5, 1996, Pages 218-222

  Sparrow, James T ^a   Monera, Oscar D ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APOLIPOPROTEIN A1; HYDROFLUORIC ACID; PEPTIDE FRAGMENT; PLANT RESIN; TRIMETHYLSILYL BROMIDE; TRIMETHYLSILYL DERIVATIVE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COMPARATIVE STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; APOLIPOPROTEIN A-I; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HYDROFLUORIC ACID; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RESINS, PLANT; TRIMETHYLSILYL COMPOUNDS;

EID: 0030237213     PISSN: 10405704     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Barany, G. and R.B. Merrifield. 1980. Solid Phase Peptide Synthesis, p. 3-284. In E. Gross and J. Meienhot'er (Eds.), The Peptides: Analysis, Synthesis, Biology. Academic Press, New York.
2. Bergot, B.J., R.L. Noble and T. Geiser. 1987 TFMSA/TFA cleavage and deprotection in SPPS, p. 97-101. In D. Theodoropolous (Eds.), Peptides 1986. Walter deGruyter and Co., Berlin.
3. Gottchalk, S., J.T. Sparrow, J. Hauer, M.P. Mims, F.E. Leland, S.L.C. Woo and L.C. Smith. 1996. A novel DNA-peptide complex for efficient gene transfer and expression in mammalian cells. Gene Ther. j:448-457.
4. Grant, G.A. 1992. Synthetic Peptides, A User's Guide. W.H. Freeman and Co., New York.
5. Hughes, J.L. and E.J. Leopold. 1993. Cleavage and deprotection of peptides from MBHA-resin with bromotrimethylsilane. Tetrahedron Lett. 34:1113-1716.
6. Hughes, J.L. and E.J. Leopold. 1995. Cleavage and deprotection of peptide resins using chloro- and bromotrialkylsilanes. Pept. Res. 8:298-300.
7. Merrifield, R.B. 1963. Solid phase peptide synthesis. I. The synthesis of a letrapeptide. J. Am. Chem. Soc. 85:2149-2154.
8. Merrifield, R.B. 1964. Solid-phase peptide synthesis. III. An improved synthesis of bradykinin. Biochemistry 3:1385-1390.
9. Reid, G.E. and R.J. Simpson. 1992. Automated solid-phase peptide synthesis: use of 2-(1H-benzotriazol-1-yl)-1,1,3,3,-tetrarnethyluronium tetrafluoroboratc for coupling of tert-butyloxycarbonyl amino acids. Anal. Biochem. 200:301-309.
10. Tam, J. and R.B. Merrifield. 1987. Strong acid deprotection of synthetic peptides: mechanisms and methods, p. 185-248. In S. Undenfriend and J. Meienhofer (Eds.), The Peptides. Academic Press, New York.
11. .Yajima, H., N. Fujii, S. Funakoshi, T. Watanabe, E. Murayama and A. Otaka. 1988. New strategy for the chemical synthesis of proteins. Tetrahedron 44:805-819.