A new method to detect response of lymphocytes to allo-antigens

Hiroshima Journal of Medical Sciences

Volumn 45, Issue 3, 1996, Pages 99-103

  Okimoto, Tatsuya ^a  

^a HIROSHIMA UNIV SCHOOL OF MEDICINE   (Japan)

Author keywords

IL 2 mRNA; MLC; RT PCR

Indexed keywords

ALLOANTIGEN; INTERLEUKIN 2; MESSENGER RNA;

ADULT; ARTICLE; COMPARATIVE STUDY; FEMALE; GENETICS; HUMAN; IMMUNOLOGY; IN VITRO STUDY; KIDNEY TRANSPLANTATION; LYMPHOCYTE; MALE; METABOLISM; METHODOLOGY; MIXED LYMPHOCYTE CULTURE; POLYMERASE CHAIN REACTION;

ADULT; FEMALE; HUMANS; INTERLEUKIN-2; ISOANTIGENS; KIDNEY TRANSPLANTATION; LYMPHOCYTE CULTURE TEST, MIXED; LYMPHOCYTES; MALE; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0030227495     PISSN: 00182052     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Asakawa, J., Satoh, C., Yamasaki, Y. and Chen, S.H. 1992. Accurate and rapid detection of heterozygous carriers of a deletion by combined polymerase chain reaction and high-performance liquid chromatography. Proc. Natl. Acad. Sci. U.S.A. 89: 9126-9130.
2. Brenner, C.A., Daniel, S. and Adler, R.R. 1991. Cytokine MAPPing: observation and quantification of cytokine mRNA in small numbers of cells using the polymerase chain reaction, p. 51-59. In F. R. Balkwill (ed.), The practical approach series. Oxford University Press, New York.
3. Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159.
4. Keuning, J.J., van den Tweel, J., Gabb, B.W., Termijtelen, A., Goulmy, E., Blokland, E., Elferink, B.G. and van Rood, J. 1975. An estimation of the recombination fraction between the MLC locus and the FOUR locus. Tissue Antigens 6: 107-115.
5. Miyajima, T., Higuchi, R., Kashiwabara, H., Yokoyama, T. and Fujimoto, S. 1980. Anti-idiotypic antibodies in a patient with a functioning renal graft. Nature 283: 306-308.
6. Naylor, M.S. and Balkwill, F.R. 1991. Northern analysis and in situ hybridization on cytokine messenger RNA, p.31-50. In F.R. Balkwill (ed.), A Practical Approach. Oxford University Press, New York.
7. Okimoto, T., Yahata, H., Fukuda, Y., Hayamizu, K., Shimatani, K. and Dohi, K. 1994. Donor specific suppressor T cells in a renal transplant recipient with a well functioning kidney. Transplant. Proc. 26: 858-860.
8. Pfeffer, P.F., Thorsby, E. and Hirschberg, H. 1983. Cell-mediated cytotoxicity toward the donor in patients with well functioning kidney grafts. Transplantation 35: 546-551.
9. Stiller, C.R., Sinclair, N.R., Abrahams, S., McGirr, D. and Ulan, R.A. 1976. Anti-donor immune responses in prediction of transplant rejection. New Eng. J. Med. 294: 978-982.
10. Thomsen, M., Morling, N., Ladefoged, J., Lokkegard, H., Jakobsen, B., Svejgaard, A. and Thaysen, J. H. 1980. MLC compatibility, HLA-D and DR typing in cadaver kidney transplantations. Scand. J. Urol. Nephrol. Suppl. 54: 32-35.
11. Umlauf, S.W., Beverly, B., Kang, S.M., Brorson, K., Tran, A.C. and Schwartz, R.H. 1993. Molecular regulation of the IL-2 gene: Rheostatic control of the immune system. Immuno. Rev. 133: 177-197.
12. Wang, A.M., Doyle, M.V. and Mark, D.F. 1989. Quantitation of mRNA by the polymerase chain reaction. Biochemistry 86: 9717-9721.
13. Wramner, L., Olausson, M., Söderström, T., Lindholm, L., Rydberg, L. and Brunger, H. 1987. Evidence of donor specific cellular suppressor activity in donor specific cell mediated lympholysis unresponsiveness in renal transplant patients. Transplantation 44: 390-395.
14. Yamamoto, Y., Fujimoto, K., Ohmura, T., Maeda, S., Shimada, K. and Onoue, K. 1985. Interleukin 2 mRNA induction in human lymphocytes: Analysis of the synergistic effect of a phorbol ester and phytohemagglutinin. J. Biochem. 98: 49-56.