Immobilization of a recombinant cutinase by entrapment and by covalent binding kinetic and stability studies

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 60, Issue 3, 1996, Pages 217-228

  Gonalves A P V ^a   Cabral, J M S ^a   Aires Barros, M R ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Covalent binding; Gel entrapment; Hydrolysis; Kinetic; Recombinant cutinase; Stability

Indexed keywords

CARBOXYLESTERASE; CUTINASE; EXCIPIENT; IMMOBILIZED ENZYME; OCTANOIC ACID DERIVATIVE; RECOMBINANT PROTEIN; SILICON DIOXIDE; TRIACYLGLYCEROL; TRIOCTANOIN;

ARTICLE; DOSE RESPONSE; ENZYME STABILITY; FUSARIUM; KINETICS; METABOLISM; PH; TEMPERATURE;

CARBOXYLIC ESTER HYDROLASES; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME STABILITY; ENZYMES, IMMOBILIZED; EXCIPIENTS; FUSARIUM; HYDROGEN-ION CONCENTRATION; KINETICS; OCTANOIC ACIDS; RECOMBINANT PROTEINS; SILICON DIOXIDE; TEMPERATURE; TRIGLYCERIDES;

EID: 0030227450     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02783585     Document Type: Article

References (2)

1. Goldberg, M. (1989), PhD thesis, Université de Compiegne, Compiegne, France.
2. Klibanov, A. M. (1979), Anal. Biochem. 93, 1-25.