Molecular anatomy of the β′ subunit of the E. coli RNA polymerase: Identification of regions involved in polymerase assembly

Genes to Cells

Volumn 1, Issue 9, 1996, Pages 819-827

  Luo, Jianying ^a,b,c   Sharif, Karim A ^a,b,d   Jin, Ruzhong ^a,e   Fujita, Nobuyuki ^b   Ishihama, Akira ^b   Krakow, Joseph S ^a  

^a CITY UNIVERSITY OF NEW YORK   (United States)

^b NATIONAL INSTITUTE OF GENETICS   (Japan)

^c BOSTON CHILDREN S HOSPITAL   (United States)

^d ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^e NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED RNA POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE DELETION; PHYSIOLOGY; PROTEIN CONFORMATION; PROTEIN FOLDING;

AMINO ACID SEQUENCE; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENE DELETION; PROTEIN CONFORMATION; PROTEIN FOLDING;

EID: 0030227080     PISSN: 13569597     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2443.1996.700269.x     Document Type: Article

References (32)

1. Ahearn, J.M., Bartolomei, M.S., Wast, M.L., Cisek, L.J. & Corden, J.L. (1987) Cloning and sequence analysis of the mouse genomic locus encoding the largest subunit of RNA polymerase II. J. Biol. Chem. 262, 10695-10705.
2. Allison, L.A., Moyle, M., Shales, M. & Ingles, C.J. (1985) Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases. Cell 42, 599-610.
3. Blatter, E.E., Ross, W., Tang, H., Gourse, R.L. & Ebright, R.H. (1994) Domain organization of RNA polymerase α subunit: C-terminal 85 amino acids constitute a domain capable of dimerization and DNA binding. Cell 78, 889-896.
4. Borukhov, S., Lee, J. & Goldfarb, A. (1991) Mapping of a contact site for the RNA 3′ terminus in the largest subunit of RNA polymerase. J. Biol. Chem. 266, 23932-23935.
5. Fukuda, R. & Ishihama, A. (1974) Subunits of RNA polymerase in function and structure; Maturation in vitro of core enzyme from Escherichia coli. J. Mol. Biol. 87, 523-540.
6. Gaal, T., Ross, W. & Blatter, E.E., et al. (1996) DNA-binding determinants of the α subunit of RNA polymerase: novel DNA-binding domain architecture. Genes Dev. 10, 16-26.
7. 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting. Proc. Natl. Acad. Sci. USA 93, 71-75.
8. Igarashi, K., Fujita, N, & Ishihama, A. (1991) Identification of a subunit assembly domain in the α subunit of Escherichia coli RNA polymerase. J. Mol. Biol. 218, 1-6.
9. Igarashi, K. & Ishihama, A. (1991) Bipartite functional map of the E. coli RNA polymerase a subunit: involvement of the c-terminal region in transcription activation by cAMP-CRP. Cell 65, 1015-1022.
10. Ishihama, A. (1981) Subunit assembly of Escherichia coli RNA polymerase. Adv. Biophys. 14, 1-35.
11. Ito, K. & Nakamura, Y. (1993) Pleiotropic effects of the rpoC10 mutation affecting the RNA polymerase β′ subunit of Escherichia coli on factor-dependent transcription termination and antitermination. Mol. Microbiol. 9, 285-93.
12. Jeon, Y.H., Negishi, T. & Shirkawa, M., et al. (1995) Solution structure of the activator contact domain of the RNA polymerase a subunit. Science 270, 1495-1497.
13. Jokerst, R.S., Weeks, J.R., Zehring, W.A. & Greenleaf, A.L. (1989) Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila. Mol. Gen. Genet. 215, 266-275.
14. Kashlev, M.A., Lee, J., Zelenskaya, K., Nikiforov, V. & Goldfarb, A. (1990) Blocking of the initiation-to-elongation transition by a transdominant RNA polymerase mutation. Science 248, 1006-1009.
15. Kashlev, M.A., Martin, E., Polyakov, A., Severinov, K., Nikiforov, V. & Goldfarb, A. (1990) Histidine-tagged RNA polymerase: dissection of the transcription cycle using immobilized enzyme. Gene 130, 9-14.
16. Krakow, J.S. & von der Helm, K. (1971) Azotobacter RNA polymerase transitions and release of σ. Cold Spring Harbor Symp. Quant. Biol. 35, 73-83.
17. Landick, R., Colwell, A. & Stewart, J. (1990) Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit β-subunit assembly into RNA polymerase. J. Bacteriol. 172, 2844-2854.
18. Lisitsyn, N.A., Monastyrskaya, G.A. & Sverdlov, E.D. (1988) Genes coding for RNA polymerase β subunit in bacteria. Structure/function analysis. Eur. J. Biochem. 177, 363-369.
19. 70 family: sequence conservation and evolutionary relationship. J. Bacteriol. 174, 3843-3849.
20. Luo, J. & Krakow, J.S. (1992) Characterization and epitope mapping of monoclonal antibodies directed against the β′ subunit of the Escherichia coli RNA polymerase. J. Biol. Chem. 267, 18175-18181.
21. Polyakov, A., Severinova, E. & Darst, S.A. (1995) Three-dimensional structure of E. coli RNA polymerase. Promoter binding and elongation conformations of the enzyme. Cell 83, 365-373.
22. Reznikoff, W.S., Siegele, D.A., Cowing, D.W. & Gross, C.A. (1985) The regulation of transcription initiation in bacteria. Annu. Rev. Genet. 19, 355-387.
23. Riftina, F., DeFalco, E. & Krakow, J.S. (1989) Monoclonal antibodies as probes of the topological arrangement of the α subunits of Escherichia coli RNA polymerase. Biochemistry 28, 3299-3305.
24. Rockwell, P., Beasley, E. & Krakow, J.S. (1985) Characterization of effects of anti-β and anti-β′ monoclonal antibodies on the activity of the RNA polymerase from Escherichia coli. Biochemistry 24, 3240-3245.
25. Sethi, V.S. & Zillig, W. (1970) Dissociation of DNA-dependent RNA polymerase from E. Coli in lithium chloride. FEBS Lett. 6, 339-342.
26. Severinov, K., Fenyo, D. & Severinova, E., et al. (1994) The σ subunit conserved region 3′ is part of '5′-face' of active center of Escherichia coli RNA polymerase. J. Biol. Chem. 269, 20826-20828.
27. Severinov, K., Markov, D. & Severinova, E., et al. (1995a) Streptolydigin-resistant mutants in an evolutionarily conserved region of the β′ subunit of Escherichia coli RNA polymerase. J. Biol. Chem. 270, 23926-23929.
28. Severinov, K., Mustaev, A., Severinova, E., Kozlov, M., Darst, S.A. & Goldfarb, A. (1995b) The β subunit Rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase. J. Biol. Chem. 270, 29428-29432.
29. Sharif, K.A. (1996) Antibodies as probes of the structure and function of the α and β′ subunits of the E. coli RNA polymerase. PhD thesis, City University of New York, New York.
30. 70 subunit of Escherichia coli RNA polymerase. Monoclonal antibodies. Localization of epitopes by peptide mapping and effects on transcription. Biochemistry 27, 5755-5762.
31. Tabor, S. & Richardson, C.C. (1985) A bacteriophage T7 RNA polymerase system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA. 82, 1074-1078.
32. Weilbaecher, R., Hebron, C., Feng, G. & Landick, R. (1994) Termination-altering amino acid substitutions in the β′ subunit of Escherichia coli RNA polymerase identify regions involved in RNA chain elongation. Genes Dev. 8, 2913-2927.