Effects of concanavalin A and phorbol myristate acetate on glutamine metabolism and proliferation of porcine intestinal intraepithelial lymphocytes

Comparative Biochemistry and Physiology - A Physiology

Volumn 114, Issue 4, 1996, Pages 363-368

  Wu, Guoyao ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

glutamine; intraepithelial lymphocytes; metabolism; pig; thymidine uptake

Indexed keywords

CONCANAVALIN A; PHORBOL 13 ACETATE 12 MYRISTATE;

AMINO ACID METABOLISM; ANIMAL TISSUE; ANTIGEN RECOGNITION; ARTICLE; CELLULAR DISTRIBUTION; IMMUNE RESPONSE; INTESTINE LYMPHATIC TISSUE; LYMPHOCYTE PROLIFERATION; LYMPHOID ORGAN; MESENTERY LYMPH NODE; NONHUMAN; PRIORITY JOURNAL; SWINE;

EID: 0030222189     PISSN: 03009629     EISSN: None     Source Type: Journal    
DOI: 10.1016/0300-9629(96)00027-8     Document Type: Article

References (24)

1. 1. Ardawi, M.S.M.; Newsholme, E.A. Glutamine metabolism in lymphocytes of the rat. Biochem. J. 212:835-842;1983.
2. 2. Barrett, T.A.; Gajeski, T.F.; Danielpour, D.; Chang, E.G.; Beagley, K.W.; Bluestone, J.A. Differential function of intestinal intraepithelial lymphocyte subsets. J. Immunol. 149: 1124-1130;1992.
3. 3. Berry, N.A.; Ase, K.; Kikkawa, U.; Kishimoto, A.; Nishizuka, Y. Human T cell activation by phorbol esters and diacylglycerol. J. Immunol. 143:1407-1413;1989.
4. 4. Dugan, M.E.R.; Knabe, D.A.; Wu, G. Glutamine and glucose metabolism in intraepithelial lymphocytes from pre-and post-weaning pigs. Comp. Bochem. Physiol. 109B:675-681;1994.
5. 5. Eagle, H. Amino acid metabolism in mammalian cell cultures. Science 130:432-437;1959.
6. 6. Ebert, E.C. Proliferative responses of human intraepithelial lymphocytes to various T cell stimuli. Gastroenterology 97: 1372-1381;1989.
7. 7. Klein, J.R. Advances in intestinal T-cell development and function. Immunol. Today 16:322-324;1995.
8. 8. Lefrancois, L. Basic aspects of intraepithelial lymphocyte immunobiology. In: Ogra, P.L.; Lamm, M.E.; McGhee, J.R.; Mesteck, J.; Strober, W.; Bienenstock, J. (eds). Handbook of mucosal immunology. San Diego: Academic Press; 1994:287-297.
9. 9. Mayrhofer, G.; Whately, R.J. Granular intraepithelial lymphocytes of the rat small intestine. Int. Archs. Allergy Appl. Immunol. 71:317-327;1983.
10. 10. McKeehan, W.L. Glycolysis, glutaminolysis and cell proliferation. Cell Biol. Int. Rep. 6:635-650;1982.
11. 11. Mosley, R.L.; Whetsell, M.; Klein, J.R. Proliferative properties of murine intestinal intraepithelial lymphocytes (IEL). Int. Immunol. 3:563-569;1991.
12. 12. Mowat, A.M. In: Margh, M.N. (ed). The cellular basis of gastrointestinal immunity. London: John Wiley; 1987.
13. 13. Mowat, A.M.I.; McInnes, I.B.; Parrott, D.M.V. Functional properties of intraepithelial lymphocytes from mouse small intestine. IV. Investigation of the proliferative capacity of IEL using phorbol ester and calcium ionophore. Immunology 66: 398-403;1989.
14. 14. Poussier, P.; Edouard, P.; Lee, C.; Binnie, M.; Julius, M. Thymus- independent development and negative selection of T cells expressing T cell receptor α/β in the intestinal epithelium: evidence for distinct circulation patterns of gut-and thymus-derived T lymphocytes. J. Exp. Med. 176:187-199;1992.
15. 15. Poussier, P.; Julius, M. Thymus independent T cell development and selection in the intestinal epithelium. Annu. Rev. Immunol. 12:521-553;1994.
16. 16. Roitt, I.; Brostoff, J.; Male, D. Immunology. New York: Harper & Row; 1989.
17. 17. Salmon, H. The intestinal and mammary immune system in pigs. Vet. immunol. Immunopathol. 17:367-388;1987.
18. 18. SAS. SAS user's guide: statistics. Cary, NC: ASA Institute Inc.; 1990.
19. 19. Szondy, Z.; Newsholme, E.A. The effect of time of addition of glutamine or nucleosides on proliferation of rat cervical lymph-node T-lymphocytes after stimulation by concanavalin A. Biochem. J. 278:471-474;1991.
20. 20. Wu, G.; Borbolla, A.G.; Knabe, D.A. The uptake of glutamine and release of arginine, citrulline and proline by the small intestine of developing pigs. J. Nutr. 124;2437-2444;1994.
21. 21. Wu, G.; Field, C.J.; Marliss, E.B. Glutamine and glucose metabolism in splenocytes and mesenteric lymph node lymphocytes. Am. J. Physiol 260:E141-E147;1991.
22. 22. Wu, G.; Field, C.J.; Marliss, E.B. Enhanced glutamine and glucose metabolism in cultured rat splenocytes stimulated by phorbol myristate plus ionomycin. Metabolism 41:982-988; 1992.
23. 23. Wu, G.; Greene, L.W. Glutamine and glucose metabolism in bovine blood lymphocytes. Comp. Biochem. Physiol. 103B: 821-825;1992.
24. 24. Wu, G.; Knabe, D.A.; Flynn, N.E. Synthesis of citrulline from glutamine in pig enterocytes. Biochem. J. 299:115-121;1994.