Human pediatric and adult ventricular cardiomyocytes in culture: Assessment of phenotypic changes with passaging

Cardiovascular Research

Volumn 32, Issue 2, 1996, Pages 362-373

  Li, Ren Ke ^a   Mickle, Donald A G ^a,b   Weisel, Richard D ^a   Carson, Susan ^a   Omar, Sue A ^a   Tumiati, Laura C ^a   Wilson, Gregory J ^a   Williams, William G ^a  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b TORONTO GENERAL HOSPITAL   (Canada)

Author keywords

Contractile proteins; Electron microscopy; Human ventricular myocytes; Phospholipids

Indexed keywords

CONTRACTILE PROTEIN;

ADULT; ARTICLE; CELL DIFFERENTIATION; CHILD; CONTROLLED STUDY; ELECTRON MICROSCOPY; HEART VENTRICLE WALL; HUMAN; HUMAN CELL; MORPHOLOGY; NORTHERN BLOTTING; PHENOTYPE; PRIORITY JOURNAL;

AGED; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL SIZE; CELLS, CULTURED; CHILD, PRESCHOOL; CREATINE KINASE; FATTY ACIDS; HUMANS; INFANT; MICROFILAMENTS; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE; MYOCARDIUM; MYOSIN HEAVY CHAINS; SARCOMERES;

EID: 0030222165     PISSN: 00086363     EISSN: None     Source Type: Journal    
DOI: 10.1016/0008-6363(96)00079-X     Document Type: Article

References (28)

1. [1] Claycomb WC, Palazzo MC. Culture of terminally differentiated adult cardiac muscle cells: /A light and scanning electron microscope study. Dev Biol 1980;80:466-482.
2. [2] Claycomb WC, Moses RL. Culture of atrial and ventricular cardiac muscle cells from the adult squirrel monkey Saimiri sciureus. Exp Cell Res 1985;161:95-100.
3. [3] Ng AC, Cheng M. Adult mammalian cardiac muscle cells in culture. Tissue Cell 1981;13(3):515-523.
4. [4] Ng AC, Cheng M, Fischman DA, Zak R. Long-term culture of adult mammalian cardiac myocytes: electron microscopic and immunofluorescent analysis of myofibrillar structure. J Mol Cell Cardiol 1981;15:301-317.
5. [5] Simpson P, Savion S. Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardial cells. Circ Res 1982;50:101-116.
6. [6] Atherton B, Meyer D, Simpson D. Assembly and remodeling of myofibrils and intercalated discs in cultured neonatal rat heart cells. J Cell Sci 1986;86:233-248.
7. [7] Goldstein MA, Claycomb WC, Schwartz A. DNA synthesis and mitosis in well-differentiated mammalian cardiocytes. Science 1974;193:212-213.
8. [8] Li R-K, Mickle DA, Weisel RD, et al. In vivo survival and function of transplanted rat cardiomyocytes. Circ Res 1996;78:283-288.
9. [9] Li R-K, Weisel RD, Williams WG, et al. Method of culturing cardiomyocytes from human pediatric ventricular myocardium. J Tissue Culture Meth 1992;14:93-100.
10. [10] Li R-K, Tumiati LC, Weisel RD, et al. Isolation of cardiomyocytes from human myocardium for primary cell cultures. J Tissue Culture Meth 1993;15:147-154.
11. [11] Mickle DAG, Li R-K, Weisel RD, et al. Water-soluble antioxidant specificity against free radical injury using cultured human ventricular myocytes and fibroblasts and saphenous vein endothelial cells. J Mol Cell Cardiol 1990;22:1297-1304.
12. [12] Franks DJ, Plamondon, J, Hamet P. An increase in adenylate cyclase activity precedes DNA synthesis in cultured vascular smooth muscle cells. J Cell Physiol 1984;119:41-45.
13. [13] Shaikh NA, Downar E. Time course of changes in porcine myocardial fatty phospholipid levels during ischemia. Circ Res 1981;49:316-325.
14. [14] Burton, K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 1956;62:315-323.
15. [15] Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
16. [16] Lehrach H, Diamon D, Wozney TM, et al. RNA molecular weight determinations by gel electrophoresis under denaturing conditions: a critical examination. Biochemistry 1977;16:4746-4750.
17. [17] Davis LG, Dibner MD, Battey JF. Formaldehyde gel for electrophoretic separation of RNA and Northern blot. In: Basic Methods in Molecular Biology. New York: Elsevier, 1986:143-146.
18. [18] Jandreski MA, Liew CC. Construction of a human ventricular cDNA library and characterization of a beta heavy chain cDNA clone. Hum Genet 1987;76:47-53.
19. [19] Smith DA, Glover JL, Townsend LE, et al. A method for the harvest, culture, and characterization of human adult atrial myocardial cells: correlation with age of donor. In Vitro Cell Dev Biol 1991;27A(12):914-920.
20. [20] Burger MM. Proteolytic enzymes initiating cell division and escape from contact inhibition of growth. Nature 1970;227:170-171.
21. [21] Masson-Pevet M, Jongsma HJ, de Bruijne J. Collagenase- and trypsin-dissociated heart cells: a comparative ultrastructural study. J Mol Cell Cardiol. 1976;8:747-757.
22. [22] Perissel B, Charbonne F, Moalic JM, et al. Initial stages of trypsinized cell culture of cardiac myoblasts: ultrastructural data. J Mol Cell Cardiol 1980;12:63-75.
23. [23] Tumiati LC, Li R-K, Weisel RD, Williams WG, et al. An in vitro model of hypertrophic cardiomyopathy (HCM). J Mol Cell Cardiol 1993;25(suppl III):A233.
24. [24] Eppenberger EM, Hauser I, Baechi T, et al. Immunocytochemical analysis of the regeneration of myofibrils in long-term cultures of adult cardiomyocytes of the rat. Dev Biol 1988;130:1-15.
25. [25] Decker ML, Behnke-Barclay M, Cook MG, et al. Cell shape and organization of the contractile apparatus in cultured adult cardiac myocytes. J Mol Cell Cardiol 1991;23:817-832.
26. 1-adrenergic receptor interaction. Circ Res 1985;56:884-894.
27. [27] Jacobson SL, Piper HM. Cell cultures of adult cardiomyocytes as models of the myocardium. J Mol Cell Cardiol 1986;18:661-678.
28. [28] Li R-K, Jia Z-Q, MD, Weisel RD et al. Cardiomyocyte transplantation into the scar tissue of the cryo-necrosed ventricle improves heart function. Ann Thorac Surg 1996, in press.