Structure of the NMDA receptor channel M2 segment inferred from the accessibility of substituted cysteines

Neuron

Volumn 17, Issue 2, 1996, Pages 343-352

  Kuner, Thomas ^a,b   Wollmuth, Lonnie P ^b   Karlin, Arthur ^c   Seeburg, Peter H ^a,b   Sakmann, Bert ^b  

^a Zentrum fur Molec Biol Heidelberg   (Germany)

^b MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^c The New York Presbyterian Hospital   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; CYSTEINE; MAGNESIUM ION; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; RECEPTOR SUBUNIT; THIOL REAGENT;

ALPHA HELIX; AMINO ACID SUBSTITUTION; ARTICLE; CHANNEL GATING; ION PERMEABILITY; OOCYTE; PATCH CLAMP; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; VOLTAGE CLAMP;

EID: 0030220889     PISSN: 08966273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0896-6273(00)80165-8     Document Type: Article

References (33)

1. Akabas, M.H., Stauffer, D.A., Xu, M., and Karlin, A. (1992). Acetylcholine receptor channel structure probed in cysteine-substitution mutants. Science 258, 307-310.
2. Akabas, M.H., Kaufmann, C., Archdeacon, P., and Karlin, A. (1994). Identification of acetylcholine receptor channel-lining residues in the entire M2 segment of the alpha subunit. Neuron 13, 919-927.
3. Arellano, R.O., Woodward, R.M., and Miledi, R. (1995). A monovalent cationic conductance that is blocked by extracellular divalent cations in Xenopus oocytes. J. Physiol. Lond. 484, 593-604.
4. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1995). Chapter 8: mutagenesis of cloned DNA. In Current Protocols in Molecular Biology. K. Janssen, ed. (New York: John Wiley and Sons, Incorporated).
5. Behe, P., Stern, P., Wyllie, D., Nassar, M., Schoepfer, R., and Colquhoun, D. (1995). Determination of NMDA NR1 subunit copy number in recombinant NMDA receptors. Proc. R. Soc. Lond. (B) 262, 205-213.
6. Bennett, J.A., and Dingledine, R. (1995). Topology profile for a glutamate receptor: three transmembrane segments and a channel-lining reentrant membrane loop. Neuron 14, 373-384.
7. Bliss, T.V.P., and Collingridge, G.L. (1993). A synaptic model of memory: long-term potentiation in the hippocampus. Nature 361, 31-39.
8. Burnashev, N., Schoepfer, R., Monyer, H., Ruppersberg, J.P., Günther, W., Seeburg, P.H., and Sakmann, B. (1992). Control by asparagine residues of calcium permeability and magnesium blockade in the NMDA receptor. Science 257, 1415-1419.
9. Burnashev, N., Zhou, Z., Neher, E., and Sakmann, B. (1995). Fractional calcium currents through recombinant GluR channels of the NMDA, AMPA and kainate receptor subtypes. J. Physiol. Lond. 485, 403-418.
10. Collingridge, G.L., and Singer, W. (1990). Excitatory amino acid receptors and synaptic plasticity.Trends Pharmacol. Sci. 11, 290-296.
11. Colquhoun, D., Jonas, P., and Sakmann, B. (1992). Action of brief pulses of glutamate on AMPA/kainate receptors in patches from different neurones of rat hippocampal slices. J. Physiol. Lond. 458, 261-287.
12. Hamill, O.P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F.J. (1981). Improved patch-clamp technique for high resolution current recording from cells and cell-free membrane patches. Pflügers Arch. 391, 85-100.
13. Hilgemann, D.W. (1995). The giant membrane patch. In Single-Channel Recording. B. Sakmann and E. Neher, eds. (New York: Plenum Press), pp. 307-326.
14. Hille, B. (1992). Ionic Channels of Excitable Membranes, Second Edition. (Sunderland, Massachusetts: Sinauer Associates, Incorporated).
15. Hollmann, M., Maron, C., and Heinemann, S. (1994). N-glycosylation site tagging suggests a three transmembrane segment topology for the glutamate receptor GluR1 Neuron 13, 1331-1343.
16. Ishii, T., Moriyoshi, K., Sugihara, H., Sakurada, K., Kadotani, H., Yokoi, M., Akazawa, C., Shigemoto, R., Mizuno, N., Masu, M., and Nakanishi, S. (1993). Molecular characterization of the family of the N-Methyl-D-Aspartate receptor subunits. J. Biol. Chem. 268, 2836-2843.
17. 2+ of N-methyl-D-aspartate-activated channels. Biophys. J. 57, 1085-1090.
18. 2+ block in NMDA receptor channels. J. Neurosci. 16, 3549-3558.
19. Kutsuwada, T., Kashiwabuchi, N., Mori, H., Sakimura, K., Kushiya, E., Araki, K., Meguro, H., Masaki, H., Kumanishi, T., Arakawa, M., and Mishina, M. (1992). Molecular diversity of the NMDA receptor channel. Nature 358, 36-41.
20. MacDermott, A.B., Mayer, M.L., Westbrook, G.L., Smith, S.J., and Barker, J.L. (1986). NMDA receptor activation increases cytoplasmic calcium concentration in cultured spinal cord neurones. Nature 321, 261-263.
21. Malenka, R.C., Kauer, J.A., Zucker, R.S., and Nicoll, R.A. (1988). Postsynaptic calcium is sufficient for potentiation of hippocampal synaptic transmission. Science 242, 81-84.
22. 2+ of NMDA responses in spinal cord neurones. Nature 309, 261-263.
23. Monyer, H., Sprengel, R., Schoepfer, R., Herb, A., Higuchi, M., Lomeli, H., Burnashev, N., Sakmann, B., and Seeburg, P.H. (1992). Heteromeric NMDA receptors: molecular and functional distinction of subtypes. Science 256, 1217-1221.
24. Moriyoshi, K., Masu, M., Ishii, T., Shigemoto, R., Mizuno, N., and Nakanishi, N. (1991). Molecular cloning and characterization of the rat NMDA receptor. Nature 354, 31-37.
25. Nicoll, R.A., and Malenka, R.C. (1995). Contrasting properties of two forms of long-term potentiation in the hippocampus. Nature 377, 115-118.
26. Nowak, L., Bregestovsky, P., Ascher, P., Herbet, A., and Prochiantz, A. (1984). Magnesium gates glutamate-activated channels in mouse central neurones. Nature 307, 462-465.
27. 2+ entry via synaptically activated NMDA receptors in the induction of long-term potentiation. Neuron 11, 811-823.
28. Stauffer, D.A., and Karlin, A. (1994). Electrostatic potential of the acetylcholine binding sites in the nicotinic receptor probed by reactions of binding-site cysteines with charged methanethiosulfonates. Biochemistry 33, 6840-6849.
29. Sun, Z.-P., Akabas, M.H., Goulding, E.H., Karlin, A., and Siegelbaum, S.A.D. (1996). Exposure of residues in the cyclic nucleotide-gated channel pore: P region structure and function in gating. Neuron 16, 141-149.
30. Villarroel, A., Burnashev, N., and Sakmann, B. (1995). Dimensions of the narrow portion of a recombinant NMDA receptor channel. Biophys. J. 68, 866-875.
31. Wo, Z.G., and Oswald, R.E. (1994). Transmembrane topology of two kainate receptor subunits revealed by N-glycosylation. Proc. Natl. Acad. Sci. USA 91, 7154-7158.
32. Wollmuth, L.P., Kuner, T., Seeburg, P.H., and Sakmann, B. (1996). Differential contribution of the NR1-and NR2A-subunits to the selectivity filter of recombinant NMDA receptor channels. J. Physiol. Lond. 491, 779-797.
33. Wood, M.W., VanDongen, H.M., and VanDongen, A.M. (1995). Structural conservation of ion conduction pathways in K channels and glutamate receptors. Proc. Natl. Acad. Sci. USA 92, 4882-4886.