A Zn(II)-binding site engineered into retinol-binding protein exhibits metal-ion specificity and allows highly efficient affinity purification with a newly designed metal ligand

Chemistry and Biology

Volumn 3, Issue 8, 1996, Pages 645-653

  Schmidt, André M ^a   Müller, Holger N ^a,b   Skerra, Arne ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

^b Biometra GmbH   (Germany)

Author keywords

chelate ligand; equilibrium dialysis; immobilized metal affinity chromatography; lipocalin; protein design

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030220473     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(96)90132-5     Document Type: Article

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