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of outstanding interest. The authors used excised and attached patch recordings to characterize large conductance, calcium-activated potassium (BK) channels in turtle hair cells. These channels together with nearby voltage-gated calcium channels (VGCCs) provide electrical tuning in the hair cells of some vertebrates. VGCCs in these cells were shown to be sensitive to DHP agonists.
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Art J, Wu Y-C, Fettiplace R. The calcium-activated potassium channels of turtle hair cells. of outstanding interest J Gen Physiol. 105:1995;49-72 The authors used excised and attached patch recordings to characterize large conductance, calcium-activated potassium (BK) channels in turtle hair cells. These channels together with nearby voltage-gated calcium channels (VGCCs) provide electrical tuning in the hair cells of some vertebrates. VGCCs in these cells were shown to be sensitive to DHP agonists.
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Art, J.1
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ATP modulation of L-type calcium channel currents in guinea pig outer hair cells
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Chen, C.1
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Two types of calcium channels in bullfrog saccular hair cells
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of special interest. DHP-sensitive VGCCs were characterized in cell-attached patch recordings from frog saccular hair cells. A smaller conductance channel that was insensitive to DHPs was also found and was suggested to be an N-type VGCC.
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Su Z-L, Jiang S-C, Gu R, Yang W-P. Two types of calcium channels in bullfrog saccular hair cells. of special interest Hear Res. 87:1995;62-68 DHP-sensitive VGCCs were characterized in cell-attached patch recordings from frog saccular hair cells. A smaller conductance channel that was insensitive to DHPs was also found and was suggested to be an N-type VGCC.
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Hear Res
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Su Z-L1
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Yang W-P4
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Kimitsuki T, Nakagawa T, Hisashi K, Komune S, Komiyama S. Single channel recordings of calcium currents in chick cochlear hair cells. Acta Otolaryngol. 114:1994;144-148.
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0028920610
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Kinetic analysis of barium currents in chick cochlear hair cells
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of special interest. The whole-cell barium current through VGCCs was characterized using a Hodgkin - Huxley kinetic model. Whole-cell currents, including the residual current following a partial DHP block, were well fit by a model assuming a single homogeneous population of VGCCs in these hair cells. ω-conotoxin GVIA, ω-agatoxin IVa and ω-conotoxin MVIIC caused no reduction of the whole-cell current.
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Zidanic M, Fuchs P. Kinetic analysis of barium currents in chick cochlear hair cells. of special interest Biophys J. 68:1995;1323-1336 The whole-cell barium current through VGCCs was characterized using a Hodgkin - Huxley kinetic model. Whole-cell currents, including the residual current following a partial DHP block, were well fit by a model assuming a single homogeneous population of VGCCs in these hair cells. ω-conotoxin GVIA, ω-agatoxin IVa and ω-conotoxin MVIIC caused no reduction of the whole-cell current.
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Zidanic, M.1
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+ channels at fluorescently labeled presynaptic active zones of hair cells
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+ channels at fluorescently labeled presynaptic active zones of hair cells. Proc Natl Acad Sci USA. 91:1994;7578-7582.
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Issa, N.1
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21
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Confocal imaging of calcium microdomains and calcium extrusion in turtle hair cells
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of special interest. The free calcium concentration during depolarization was estimated to reach 85μM near presynaptic release sites in turtle hair cells. The decay of calcium-induced fluorescence suggested that both diffusion and active extrusion regulate calcium that enters through VGCCs. Prolonged calcium loading resulted in the activation of small conductance, calcium-activated potassium (SK) channels that were blocked by apamin.
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Tucker T, Fettiplace R. Confocal imaging of calcium microdomains and calcium extrusion in turtle hair cells. of special interest Neuron. 15:1995;1323-1335 The free calcium concentration during depolarization was estimated to reach 85μM near presynaptic release sites in turtle hair cells. The decay of calcium-induced fluorescence suggested that both diffusion and active extrusion regulate calcium that enters through VGCCs. Prolonged calcium loading resulted in the activation of small conductance, calcium-activated potassium (SK) channels that were blocked by apamin.
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Tucker, T.1
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Lenzi D, Roberts W. Calcium signaling in hair cells: multiple roles in a compact cell. Curr Opin Neurobiol. 4:1994;496-502.
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Lenzi, D.1
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23
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0029108205
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A kinetic description of the calcium-activated potassium channel and its application to electrical tuning of hair cells
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of outstanding interest. Detailed characterizations of voltage-gated and calcium-activated channels were used to reconstruct the electrical tuning and related forms of excitability in hair cells of turtle and chick.
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Wu Y-C, Art J, Goodman M, Fettiplace R. A kinetic description of the calcium-activated potassium channel and its application to electrical tuning of hair cells. of outstanding interest Prog Biophys Mol Biol. 63:1995;131-158 Detailed characterizations of voltage-gated and calcium-activated channels were used to reconstruct the electrical tuning and related forms of excitability in hair cells of turtle and chick.
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Prog Biophys Mol Biol
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Wu Y-C1
Art, J.2
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Fettiplace, R.4
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24
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0030066648
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A developmental model for generating frequency maps in the reptilian and avian cochleas
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of outstanding interest. The properties of calcium-activated potassium channels were used to predict the intercellular variations that underlie the tonotopic organization of the basilar papilla. This model sets the theoretical groundwork for investigations into the molecular basis of BK channel differentiation.
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Wu Y-C, Fettiplace R. A developmental model for generating frequency maps in the reptilian and avian cochleas. of outstanding interest Biophys J. 70:1996;2557-2570 The properties of calcium-activated potassium channels were used to predict the intercellular variations that underlie the tonotopic organization of the basilar papilla. This model sets the theoretical groundwork for investigations into the molecular basis of BK channel differentiation.
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Biophys J
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Wu Y-C1
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Position-dependent expression of potassium currents by chick cochlear hair cells
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Murrow B. Position-dependent expression of potassium currents by chick cochlear hair cells. J Physiol (Lond). 480:1994;247-259.
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Fisher F. Quantitative analysis of the innervation pattern of the chicken basilar papilla. Hear Res. 61:1992;167-178.
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Fisher, F.1
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27
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0029147815
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+ channel cloned from the chick inner ear
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of outstanding interest. An inward rectifier potassium channel was cloned from chick cochlear cDNA (clRK1) and was found to be highly homologous to the mouse inward rectifier channel (IRK1). RT-PCR from microdissected tissues showed that the clRK1 transcript was prominent in the apical (low-frequency) half of the basilar papilla, in agreement with the distribution of the native inward rectifier current.
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+ channel cloned from the chick inner ear. of outstanding interest J Biol Chem. 270:1995;19238-19245 An inward rectifier potassium channel was cloned from chick cochlear cDNA (clRK1) and was found to be highly homologous to the mouse inward rectifier channel (IRK1). RT-PCR from microdissected tissues showed that the clRK1 transcript was prominent in the apical (low-frequency) half of the basilar papilla, in agreement with the distribution of the native inward rectifier current.
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Direct measurement of the action of acetylcholine on isolated outer hair cells of the guinea pig cochlea
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Housley G, Ashmore J. Direct measurement of the action of acetylcholine on isolated outer hair cells of the guinea pig cochlea. Proc R Soc Lond (Biol). 244:1991;161-167.
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Kakehata S, Nakagawa T, Takasaka T, Akaike N. Cellular mechanism of acetylcholine-induced response in dissociated outer hair cells of guinea-pig cochlea. J Physiol (Lond). 463:1993;227-244.
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Erostegui C, Norris C, Bobbin R. In vitro pharmacologic characterization of a cholinergic receptor on outer hair cells. Hear Res. 74:1994;135-147.
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Martin A, Fuchs P. The dependence of calcium-activated potassium currents on membrane potential. Proc Soc Lond (Biol). 250:1992;71-76.
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Martin, A.1
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0029920082
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Ionic dependence and agonist preference of an acetylcholine receptor in hair cells
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of special interest. The ligand-gated cation current of chick cochlear hair cells was studied in isolation. The underlying channels have a complex dependence on extracellular calcium but appear to be permeable to it.
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McNiven A, Yuhas W, Fuchs P. Ionic dependence and agonist preference of an acetylcholine receptor in hair cells. of special interest Aud Neurosci. 2:1996;63-77 The ligand-gated cation current of chick cochlear hair cells was studied in isolation. The underlying channels have a complex dependence on extracellular calcium but appear to be permeable to it.
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Aud Neurosci
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McNiven, A.1
Yuhas, W.2
Fuchs, P.3
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46
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0029933267
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Acetylcholine activates two currents in guinea-pig outer hair cells
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of outstanding interest. This paper describes the sequential activation by ACh of a cation current and then a calcium-dependent potassium current in mammalian cochlear hair cells. This provides direct evidence that mammalian hair cells employ a ligand-gated cation channel subunit similar to α9 to mediate efferent inhibition.
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Evans M. Acetylcholine activates two currents in guinea-pig outer hair cells. of outstanding interest J Physiol (Lond). 491:1996;563-578 This paper describes the sequential activation by ACh of a cation current and then a calcium-dependent potassium current in mammalian cochlear hair cells. This provides direct evidence that mammalian hair cells employ a ligand-gated cation channel subunit similar to α9 to mediate efferent inhibition.
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J Physiol (Lond)
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Evans, M.1
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47
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0030006225
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Acetylcholine-induced potassium current of guinea pig outer hair cells: Its dependence on a calcium influx through nicotinic-like receptors
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of outstanding interest. A cation current and a potassium current were elicited by ACh from mammalian cochlear hair cells. Very strong similarities to the ACh response of non-mammalian hair cells were established, including a complex dependence on extracellular calcium and an outward rectification of the cation current's voltage relation.
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Blanchet C, Erostegui C, Sugasawa M, Dulon D. Acetylcholine-induced potassium current of guinea pig outer hair cells: its dependence on a calcium influx through nicotinic-like receptors. of outstanding interest J Neurosci. 16:1996;2574-2584 A cation current and a potassium current were elicited by ACh from mammalian cochlear hair cells. Very strong similarities to the ACh response of non-mammalian hair cells were established, including a complex dependence on extracellular calcium and an outward rectification of the cation current's voltage relation.
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J Neurosci
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Blanchet, C.1
Erostegui, C.2
Sugasawa, M.3
Dulon, D.4
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48
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0028171296
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Elgoyhen A, Johnson D, Boulter J, Vetter D, Heinemann S. α9 an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells. Cell. 79:1994;705-715.
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Elgoyhen, A.1
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49
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0028971162
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Cell-specific expression of the α9 nACh receptor subunit in auditory hair cells revealed by single-cell RT-PCR
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of special interest. Messenger RNA was collected from single cells of the rat cochlea transcribed to cDNA and amplified to confirm that α9, the novel ACh receptor subunit (see [48]), is also expressed in hair cells.
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Glowatzki E, Wild K, Brandle U, Fakler G, Fakler B, Zenner H, Ruppersberg J. Cell-specific expression of the α9 nACh receptor subunit in auditory hair cells revealed by single-cell RT-PCR. of special interest Proc R Soc Lond (Biol). 262:1995;141-147 Messenger RNA was collected from single cells of the rat cochlea transcribed to cDNA and amplified to confirm that α9, the novel ACh receptor subunit (see [48]), is also expressed in hair cells.
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Glowatzki, E.1
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Fakler, G.4
Fakler, B.5
Zenner, H.6
Ruppersberg, J.7
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50
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0029123950
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Ligand-binding properties of an unusual nicotinic acetylcholine receptor subtype on isolated outer hair cells from guinea pig cochlea
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of special interest. A detailed description of nicotinic-binding sites on guinea pig cochlear hair cells was made using radiolabelled ligands. The authors used ligand-affinity analysis to demonstrate that the hair cell ACh receptor differs from other known nicotinic receptor types.
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Lawoko G, Jarlebark L, Heilbronn E. Ligand-binding properties of an unusual nicotinic acetylcholine receptor subtype on isolated outer hair cells from guinea pig cochlea. of special interest Neurosci Lett. 195:1995;64-68 A detailed description of nicotinic-binding sites on guinea pig cochlear hair cells was made using radiolabelled ligands. The authors used ligand-affinity analysis to demonstrate that the hair cell ACh receptor differs from other known nicotinic receptor types.
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Neurosci Lett
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Lawoko, G.1
Jarlebark, L.2
Heilbronn, E.3
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Nicotinic acetylcholine receptor subunits expressed in rat cochlea detected by the polymerase chain reaction
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Housley G, Batcher S, Kraft M, Ryan A. Nicotinic acetylcholine receptor subunits expressed in rat cochlea detected by the polymerase chain reaction. Hear Res. 75:1994;47-53.
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Housley, G.1
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Ryan, A.4
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Analysis of nicotine acetylcholine receptor subunits in the cochlea of the mouse
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of special interest. A catalogue of nicotinic receptor subunits in cochlear tissue was made using RT-PCR with specific primers. This work suggests additional candidate subunits that might contribute to the hair cell ACh receptor.
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Drescher D, Khan K, Green G, Morley B, Beisel K, Kaul H, Gordon D, Gupta A, Drescher M, Barretto R. Analysis of nicotine acetylcholine receptor subunits in the cochlea of the mouse. of special interest Comp Biochem Physiol. 112:1995;267-273 A catalogue of nicotinic receptor subunits in cochlear tissue was made using RT-PCR with specific primers. This work suggests additional candidate subunits that might contribute to the hair cell ACh receptor.
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(1995)
Comp Biochem Physiol
, vol.112
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Drescher, D.1
Khan, K.2
Green, G.3
Morley, B.4
Beisel, K.5
Kaul, H.6
Gordon, D.7
Gupta, A.8
Drescher, M.9
Barretto, R.10
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53
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The role of calcium in hair cell transduction
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D. Corey, Roper S. New York: Rockefeller University Press. (Society of General Physiologists Series, vol 47.]
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Fettiplace R. The role of calcium in hair cell transduction. Corey D, Roper S. Sensory Transduction. 1992;343-356 Rockefeller University Press, New York. (Society of General Physiologists Series, vol 47.].
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Sensory Transduction
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Fettiplace, R.1
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54
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A novel cholinergic `slow effect' of efferent stimulation on cochlear potentials in the guinea pig
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of outstanding interest. In guinea pig cochlea, prolonged efferent activity causes inhibitory effects with a much longer time course of build-up and decay than those resulting from single efferent shocks. Both fast and slow effects are sensitive to the same pharmacological blockers.
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Sridhar T, Liberman M, Brown M, Sewell W. A novel cholinergic `slow effect' of efferent stimulation on cochlear potentials in the guinea pig. of outstanding interest J Neurosci. 15:1995;3667-3678 In guinea pig cochlea, prolonged efferent activity causes inhibitory effects with a much longer time course of build-up and decay than those resulting from single efferent shocks. Both fast and slow effects are sensitive to the same pharmacological blockers.
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(1995)
J Neurosci
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Sridhar, T.1
Liberman, M.2
Brown, M.3
Sewell, W.4
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